PMID- 33144545
OWN - NLM
STAT- MEDLINE
DCOM- 20210706
LR  - 20221214
IS  - 1738-8872 (Electronic)
IS  - 1017-7825 (Print)
IS  - 1017-7825 (Linking)
VI  - 31
IP  - 1
DP  - 2021 Jan 28
TI  - Hepatitis B Virus DNA Polymerase Displays an Anti-Apoptotic Effect by Interacting 
      with Elongation Factor-1 Alpha-2 in Hepatoma Cells.
PG  - 16-24
LID - 10.4014/jmb.2002.02039 [doi]
AB  - Hepatitis B virus (HBV) genome P-encoded protein HBV DNA polymerase (Pol) has 
      long been known as a reverse transcriptase during HBV replication. In this study, 
      we investigated the impact of HBV Pol on host cellular processes, mainly 
      apoptosis, and the underlying mechanisms. We showed a marked reduction in 
      apoptotic rates in the HBV Pol-expressed HepG2 cells compared to controls. 
      Moreover, a series of assays, i.e., yeast two-hybrid, GST pull-down, 
      co-immunoprecipitation, and confocal laser scanning microscopy, identified the 
      host factor eEF1A2 to be associated with HBV Pol. Furthermore, knockdown of 
      eEF1A2 gene by siRNA abrogated the HBV Pol-mediated anti-apoptotic effect with 
      apoptosis induced by endoplasmatic reticulum (ER) stress-inducer thapsigargin 
      (TG), thus suggesting that the host factor eEF1A2 is essential for HBV Pol's 
      anti-apoptosis properties. Our findings have revealed a novel role for HBV Pol in 
      its modulation of apoptosis through integrating with eEF1A2.
FAU - Niu, Xianli
AU  - Niu X
AD  - Department of Biochemistry and Molecular Biology, Zunyi Medical University, 
      Zhuhai, Guangdong 519041, P.R. China.
AD  - Key Laboratory of Genetic Engineering and Medicine, Key Laboratory of Viral 
      Biology, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
FAU - Nong, Shirong
AU  - Nong S
AD  - Key Laboratory of Genetic Engineering and Medicine, Key Laboratory of Viral 
      Biology, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
FAU - Gong, Junyuan
AU  - Gong J
AD  - Key Laboratory of Genetic Engineering and Medicine, Key Laboratory of Viral 
      Biology, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
FAU - Zhang, Xin
AU  - Zhang X
AD  - Key Laboratory of Genetic Engineering and Medicine, Key Laboratory of Viral 
      Biology, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
FAU - Tang, Hui
AU  - Tang H
AD  - Key Laboratory of Genetic Engineering and Medicine, Key Laboratory of Viral 
      Biology, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
FAU - Zhou, Tianhong
AU  - Zhou T
AD  - Key Laboratory of Genetic Engineering and Medicine, Key Laboratory of Viral 
      Biology, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
FAU - Li, Wei
AU  - Li W
AD  - Key Laboratory of Genetic Engineering and Medicine, Key Laboratory of Viral 
      Biology, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - J Microbiol Biotechnol
JT  - Journal of microbiology and biotechnology
JID - 9431852
RN  - 0 (EEF1A2 protein, human)
RN  - 0 (Gene Products, pol)
RN  - 0 (P protein, Hepatitis B virus)
RN  - 0 (Peptide Elongation Factor 1)
RN  - 0 (Peptide Elongation Factors)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Carcinoma, Hepatocellular/*virology
MH  - DNA-Directed DNA Polymerase/genetics/*metabolism/pharmacology
MH  - Gene Products, pol
MH  - Hep G2 Cells
MH  - Hepatitis B virus/*enzymology/genetics
MH  - Humans
MH  - Liver Neoplasms/*virology
MH  - Peptide Elongation Factor 1/genetics/*metabolism
MH  - Peptide Elongation Factors/metabolism
MH  - RNA, Small Interfering
PMC - PMC9705884
OTO - NOTNLM
OT  - HBV DNA polymerase
OT  - Hepatitis B virus
OT  - apoptosis
OT  - chronic hepatitis B
OT  - eEF1A2
COIS- Conflict of Interest The authors have no financial conflicts of interest to 
      declare.
EDAT- 2020/11/05 06:00
MHDA- 2021/07/07 06:00
PMCR- 2020/10/30
CRDT- 2020/11/04 05:37
PHST- 2020/02/20 00:00 [received]
PHST- 2020/09/07 00:00 [revised]
PHST- 2020/10/21 00:00 [accepted]
PHST- 2020/11/05 06:00 [pubmed]
PHST- 2021/07/07 06:00 [medline]
PHST- 2020/11/04 05:37 [entrez]
PHST- 2020/10/30 00:00 [pmc-release]
AID - jmb.2002.02039 [pii]
AID - jmb-31-1-16 [pii]
AID - 10.4014/jmb.2002.02039 [doi]
PST - ppublish
SO  - J Microbiol Biotechnol. 2021 Jan 28;31(1):16-24. doi: 10.4014/jmb.2002.02039.