PMID- 33147292
OWN - NLM
STAT- MEDLINE
DCOM- 20210120
LR  - 20231213
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 15
IP  - 11
DP  - 2020
TI  - Mitogen activated protein kinase (MAPK)-regulated genes with predicted signal 
      peptides function in the Glycine max defense response to the root pathogenic 
      nematode Heterodera glycines.
PG  - e0241678
LID - 10.1371/journal.pone.0241678 [doi]
LID - e0241678
AB  - Glycine max has 32 mitogen activated protein kinases (MAPKs), nine of them 
      exhibiting defense functions (defense MAPKs) to the plant parasitic nematode 
      Heterodera glycines. RNA seq analyses of transgenic G. max lines overexpressing 
      (OE) each defense MAPK has led to the identification of 309 genes that are 
      increased in their relative transcript abundance by all 9 defense MAPKs. Here, 71 
      of those genes are shown to also have measurable amounts of transcript in H. 
      glycines-induced nurse cells (syncytia) produced in the root that are undergoing 
      a defense response. The 71 genes have been grouped into 7 types, based on their 
      expression profile. Among the 71 genes are 8 putatively-secreted proteins that 
      include a galactose mutarotase-like protein, pollen Ole e 1 allergen and extensin 
      protein, endomembrane protein 70 protein, O-glycosyl hydrolase 17 protein, 
      glycosyl hydrolase 32 protein, FASCICLIN-like arabinogalactan protein 17 
      precursor, secreted peroxidase and a pathogenesis-related thaumatin protein. 
      Functional transgenic analyses of all 8 of these candidate defense genes that 
      employ their overexpression and RNA interference (RNAi) demonstrate they have a 
      role in defense. Overexpression experiments that increase the relative transcript 
      abundance of the candidate defense gene reduces the ability that the plant 
      parasitic nematode Heterodera glycines has in completing its life cycle while, in 
      contrast, RNAi of these genes leads to an increase in parasitism. The results 
      provide a genomic analysis of the importance of MAPK signaling in relation to the 
      secretion apparatus during the defense process defense in the G. max-H. glycines 
      pathosystem and identify additional targets for future studies.
FAU - Niraula, Prakash M
AU  - Niraula PM
AD  - Department of Biological Sciences, Mississippi State University, Mississippi 
      State, MS, United States of America.
FAU - Sharma, Keshav
AU  - Sharma K
AD  - Department of Biological Sciences, Mississippi State University, Mississippi 
      State, MS, United States of America.
FAU - McNeece, Brant T
AU  - McNeece BT
AD  - Department of Biological Sciences, Mississippi State University, Mississippi 
      State, MS, United States of America.
FAU - Troell, Hallie A
AU  - Troell HA
AD  - Department of Biological Sciences, Mississippi State University, Mississippi 
      State, MS, United States of America.
FAU - Darwish, Omar
AU  - Darwish O
AD  - Department of Mathematics and Computer Science, Texas Women's University, Denton, 
      TX, United States of America.
FAU - Alkharouf, Nadim W
AU  - Alkharouf NW
AD  - Department of Computer and Information Sciences, Towson University, Towson, MD, 
      United States of America.
FAU - Lawrence, Katherine S
AU  - Lawrence KS
AD  - Department of Entomology and Plant Pathology, Auburn University, Auburn, AL, 
      United States of America.
FAU - Klink, Vincent P
AU  - Klink VP
AUID- ORCID: 0000-0002-7167-4032
AD  - Department of Biological Sciences, Mississippi State University, Mississippi 
      State, MS, United States of America.
AD  - Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, 
      Mississippi State University, Mississippi State, MS, United States of America.
AD  - Center for Computational Sciences High Performance Computing Collaboratory, 
      Mississippi State University, Starkville, MS, United States of America.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201104
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Protein Sorting Signals)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Computational Biology
MH  - Gene Ontology
MH  - Mitogen-Activated Protein Kinases/genetics/*metabolism
MH  - Plant Diseases/genetics/*parasitology
MH  - Plant Roots/enzymology/*metabolism/parasitology
MH  - Protein Sorting Signals/genetics/*physiology
MH  - RNA Interference
MH  - Glycine max/enzymology/*metabolism/*parasitology
PMC - PMC7641413
COIS- No authors have competing interests. The authors have declared that no competing 
      interests exist.
EDAT- 2020/11/05 06:00
MHDA- 2021/01/21 06:00
PMCR- 2020/11/04
CRDT- 2020/11/04 17:11
PHST- 2020/07/19 00:00 [received]
PHST- 2020/10/19 00:00 [accepted]
PHST- 2020/11/04 17:11 [entrez]
PHST- 2020/11/05 06:00 [pubmed]
PHST- 2021/01/21 06:00 [medline]
PHST- 2020/11/04 00:00 [pmc-release]
AID - PONE-D-20-22386 [pii]
AID - 10.1371/journal.pone.0241678 [doi]
PST - epublish
SO  - PLoS One. 2020 Nov 4;15(11):e0241678. doi: 10.1371/journal.pone.0241678. 
      eCollection 2020.