PMID- 33147372
OWN - NLM
STAT- MEDLINE
DCOM- 20210706
LR  - 20210706
IS  - 1365-2443 (Electronic)
IS  - 1356-9597 (Linking)
VI  - 26
IP  - 1
DP  - 2021 Jan
TI  - ISG20 and nuclear exosome promote destabilization of nascent transcripts for 
      spliceosomal U snRNAs and U1 variants.
PG  - 18-30
LID - 10.1111/gtc.12817 [doi]
AB  - Primary RNA transcripts are processed in a plethora of ways to become mature 
      functional forms. In one example, human spliceosomal U snRNAs are matured at 
      their 3'-end by an exonuclease termed TOE1. This process is important because 
      mutations in TOE1 gene can cause a human genetic disease, pontocerebellar 
      hypoplasia (PCH). Nevertheless, TOE1 may not be the only maturation exonuclease 
      for U snRNAs in the cell. Here, we biochemically identify two exonucleolytic 
      factors, Interferon-stimulated gene 20-kDa protein (ISG20) and the nuclear 
      exosome as such candidates, using a newly developed in vitro system that 
      recapitulates 3'-end maturation of U1 snRNA. However, extensive 3'-end sequencing 
      of endogenous U1 snRNA of the knockdown (KD) cells revealed that these factors 
      are not the maturation factors per se. Instead, the nascent transcripts of the 
      spliceosomal U snRNAs as well as of unstable U1 variants were found to increase 
      in quantity upon KD of the factors. These results indicated that ISG20 and the 
      nuclear exosome promote the degradation of nascent spliceosomal U snRNAs and U1 
      variants, and therefore implied their role in the quality control of newly 
      synthesized U snRNAs.
CI  - (c) 2020 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.
FAU - Kawamoto, Takahito
AU  - Kawamoto T
AUID- ORCID: 0000-0002-0532-9214
AD  - Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan.
FAU - Yoshimoto, Rei
AU  - Yoshimoto R
AD  - Department of Applied Biological Sciences, Faculty of Agriculture, Setsunan 
      University, Hirakata, Japan.
FAU - Taniguchi, Ichiro
AU  - Taniguchi I
AD  - Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan.
FAU - Kitabatake, Makoto
AU  - Kitabatake M
AD  - Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan.
FAU - Ohno, Mutsuhito
AU  - Ohno M
AD  - Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan.
LA  - eng
GR  - 25251004/Ministry of Education, Culture, Sports, Science and Technology/
GR  - 26113004/Ministry of Education, Culture, Sports, Science and Technology/
PT  - Journal Article
DEP - 20201202
PL  - England
TA  - Genes Cells
JT  - Genes to cells : devoted to molecular & cellular mechanisms
JID - 9607379
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Small Nuclear)
RN  - 0 (TOE1 protein, human)
RN  - 0 (U1 small nuclear RNA)
RN  - EC 3.1.- (Exoribonucleases)
RN  - EC 3.1.- (ISG20 protein, human)
SB  - IM
MH  - Cell Nucleus/metabolism
MH  - Exoribonucleases/genetics/*metabolism
MH  - Exosomes/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Nuclear Proteins/genetics/metabolism
MH  - RNA Stability
MH  - RNA, Small Nuclear/genetics/*metabolism
MH  - Spliceosomes/*metabolism
OTO - NOTNLM
OT  - ISG20
OT  - TOE1
OT  - U1 variants
OT  - exosome
OT  - spliceosomal U snRNAs
EDAT- 2020/11/05 06:00
MHDA- 2021/07/07 06:00
CRDT- 2020/11/04 17:12
PHST- 2020/10/09 00:00 [received]
PHST- 2020/10/29 00:00 [revised]
PHST- 2020/10/29 00:00 [accepted]
PHST- 2020/11/05 06:00 [pubmed]
PHST- 2021/07/07 06:00 [medline]
PHST- 2020/11/04 17:12 [entrez]
AID - 10.1111/gtc.12817 [doi]
PST - ppublish
SO  - Genes Cells. 2021 Jan;26(1):18-30. doi: 10.1111/gtc.12817. Epub 2020 Dec 2.