PMID- 33147433
OWN - NLM
STAT- MEDLINE
DCOM- 20210202
LR  - 20210202
IS  - 1096-0945 (Electronic)
IS  - 0014-4800 (Linking)
VI  - 117
DP  - 2020 Dec
TI  - Nitroglycerin ameliorates sperm parameters, oxidative stress and testicular 
      injury following by testicular torsion/detorsion in male rats.
PG  - 104563
LID - S0014-4800(20)30840-6 [pii]
LID - 10.1016/j.yexmp.2020.104563 [doi]
AB  - PURPOSE: This study was designed to determine the probable protection mechanisms 
      of nitroglycerin, a widely used medication for treatment of heart failure and 
      angina, in amelioration of testicular ischemia/reperfusion damage. METHODS: 24 
      adult male rats were randomly divided into three equal groups; with eight rats in 
      each group: Group 1 (Sham) was sham-operated. Group 2 (T_D): 2 h testicular 
      torsion was induced, afterward detorsion was performed and maintained for 2 h. 
      Group 3 (NG): Nitroglycerin was administered immediately after detorsion. Sperm 
      quality parameters such as viability, motility, morphology, and concentration, 
      levels of antioxidant enzymes (glutathione peroxidase (GPX), catalase (CAT), and 
      total antioxidant capacity (TAC)), and amount of malondialdehyde (MDA) in the 
      blood plasma were examined in each group, thereafter histopathological parameters 
      including germinal epithelial cell thickness (GECT), mean seminiferous tubular 
      diameter (MSTD), Johnson's score and Cosentino's score were assessed. RESULTS: 
      Testicular T_D significantly reduced sperm viability, motility, and normal 
      morphology, whereas the NG administration remarkably increased the percentage of 
      live, motile, and normal spermatozoa (p < 0.05). Levels of GPx, CAT, and TAC 
      significantly reduced and the MDA level significantly increased in the T_D group 
      in comparison to the sham group (p < 0.05). The NG treated group demonstrated 
      significantly reduced MDA concentrations as well as elevated levels of GPx and 
      CAT compared to the T_D group (p < 0.05). Induction of testicular torsion 
      significantly reduced Johnson's score, GESCT (mum), and MSTD (mum), and remarkably 
      increased the Cosentino's score (P < 0.05), while NG injection significantly 
      increased Johnson's score, GESCT (mum), and MSTD (mum) and reduced the Cosentino's 
      score (P < 0.05). CONCLUSION: According to the findings in this research, 
      nitroglycerin was able to protect the testicular tissue from ischemia-reperfusion 
      damage caused by induced torsion/detorsion.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Raisi, Abbas
AU  - Raisi A
AD  - Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan 
      University, Khorramabad, Iran. Electronic address: raisi.a@lu.ac.ir.
FAU - Kheradmand, Arash
AU  - Kheradmand A
AD  - Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan 
      University, Khorramabad, Iran.
FAU - Farjanikish, Ghasem
AU  - Farjanikish G
AD  - Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, 
      Khorramabad, Iran.
FAU - Davoodi, Farshid
AU  - Davoodi F
AD  - Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan 
      University, Khorramabad, Iran.
FAU - Taheri, Shayan
AU  - Taheri S
AD  - Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan 
      University, Khorramabad, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201102
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (Antioxidants)
RN  - 0 (Protective Agents)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - G59M7S0WS3 (Nitroglycerin)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Apoptosis/drug effects
MH  - Catalase/metabolism
MH  - Glutathione Peroxidase/metabolism
MH  - Humans
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Nitroglycerin/*pharmacology
MH  - Oxidative Stress/drug effects
MH  - Protective Agents/pharmacology
MH  - Rats
MH  - Reperfusion Injury/pathology/prevention & control
MH  - Spermatic Cord Torsion/*drug therapy/pathology
MH  - Spermatozoa/drug effects
MH  - Testicular Diseases/*drug therapy/metabolism/pathology
MH  - Testis/*drug effects/injuries/pathology
OTO - NOTNLM
OT  - Ischemia-reperfusion
OT  - Nitroglycerin
OT  - Oxidative stress
OT  - Sperm parameters
OT  - Testicular torsion detorsion
EDAT- 2020/11/05 06:00
MHDA- 2021/02/03 06:00
CRDT- 2020/11/04 20:07
PHST- 2020/04/21 00:00 [received]
PHST- 2020/08/18 00:00 [revised]
PHST- 2020/10/28 00:00 [accepted]
PHST- 2020/11/05 06:00 [pubmed]
PHST- 2021/02/03 06:00 [medline]
PHST- 2020/11/04 20:07 [entrez]
AID - S0014-4800(20)30840-6 [pii]
AID - 10.1016/j.yexmp.2020.104563 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2020 Dec;117:104563. doi: 10.1016/j.yexmp.2020.104563. Epub 2020 
      Nov 2.