PMID- 33148380
OWN - NLM
STAT- MEDLINE
DCOM- 20201106
LR  - 20201106
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 36
IP  - 10
DP  - 2020 Oct
TI  - [Inorganic arsenic exposure suppresses immunomodulatory effect of renal CD4(+) T 
      lymphocytes in mice].
PG  - 871-876
AB  - Objective To investigate the effects of inorganic arsenic exposure on the 
      differentiation of renal CD4(+)T lymphocytes and the possible mechanism. Methods 
      Female C57BL/6 mice were randomly divided into control group, (2.5, 5, 10) mg/kg 
      NaAsO(2) exposure groups, 10 mice in each group. As was administered once 
      intragastrically for 24 hours, and control mice were treated with normal saline. 
      Real-time fluorescence quantitative PCR was used to detect T helper type 1 (Th1) 
      cell-specific transcription factor T-box expressed in T cells (T-bet) and IFN-gamma, 
      Th2 cell-specific transcription factor GATA-binding protein 3 (GATA3) and 
      interleukin 4 (IL-4), Th17 cell-specific transcription factor retinoic acid 
      related orphan nuclear receptor gammat (ROR-gammat) and cytokine IL-22, regulatory T 
      cells (Tregs)-specific transcription factor forkhead box P3 (FOXP3) and cytokine 
      transforming growth factor-beta (TGF-beta) mRNA levels. We used commercial kits to 
      detect catalase (CAT) activity and total antioxidant capacity (T-AOC) in serum as 
      well as renal malondialdehyde (MDA) and superoxide dismutase (SOD). Results 
      Compared with the control group, the body mass, renal mass and kidney index of 
      the mice in all arsenic-treated groups have no significant changes. The levels of 
      the master transcription factors T-bet, GATA3, ROR-gammat and FOXP3 as well as 
      related cytokines IFN-gamma, IL-4, IL-22 and TGF-beta of Th1, Th2, Th17 cells and Tregs 
      decreased in the arsenic-treated groups. Serum CAT activity and T-AOC level in 
      the arsenic-treated mice dropped greatly. In addition, arsenic markedly increased 
      renal MDA level while decreased SOD activity. Conclusion Inorganic arsenic 
      exposure can suppress renal T cell subpopulation function and induce renal 
      oxidative injure.
FAU - Li, Jingyu
AU  - Li J
AD  - Department of Toxicology, Public Health, Shenyang Medical College, Shenyang 
      110034, China.
FAU - Li, Xin
AU  - Li X
AD  - Environment and Non-Communicable Disease Research Center, School of Public 
      Health, China Medical University, Shenyang 110122, China.
FAU - Li, Mei
AU  - Li M
AD  - Department of Toxicology, Public Health, Shenyang Medical College, Shenyang 
      110034, China.
FAU - Wang, Shuwen
AU  - Wang S
AD  - Department of Toxicology, Public Health, Shenyang Medical College, Shenyang 
      110034, China.
FAU - Liu, Qitong
AU  - Liu Q
AD  - Department of Toxicology, Public Health, Shenyang Medical College, Shenyang 
      110034, China.
FAU - Huang, Yucheng
AU  - Huang Y
AD  - Department of Toxicology, Public Health, Shenyang Medical College, Shenyang 
      110034, China.
FAU - Yang, Lanxin
AU  - Yang L
AD  - Department of Toxicology, Public Health, Shenyang Medical College, Shenyang 
      110034, China.
FAU - Duan, Xiaoxu
AU  - Duan X
AD  - Department of Toxicology, Public Health, Shenyang Medical College, Shenyang 
      110034, China. *Corresponding author, E-mail: duanxiaoxu@symc.edu.cn.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Cytokines)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Foxp3 protein, mouse)
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (Gata3 protein, mouse)
RN  - N712M78A8G (Arsenic)
SB  - IM
MH  - Animals
MH  - Arsenic/*toxicity
MH  - Cytokines/metabolism
MH  - Female
MH  - Forkhead Transcription Factors/metabolism
MH  - GATA3 Transcription Factor/metabolism
MH  - Kidney/*drug effects/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Oxidative Stress
MH  - T-Lymphocytes, Regulatory/*drug effects
MH  - Th1 Cells/*drug effects
MH  - Th2 Cells/*drug effects
EDAT- 2020/11/06 06:00
MHDA- 2020/11/11 06:00
CRDT- 2020/11/05 05:37
PHST- 2020/11/05 05:37 [entrez]
PHST- 2020/11/06 06:00 [pubmed]
PHST- 2020/11/11 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2020 Oct;36(10):871-876.