PMID- 33149998
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201106
IS  - 2156-7085 (Print)
IS  - 2156-7085 (Electronic)
IS  - 2156-7085 (Linking)
VI  - 11
IP  - 10
DP  - 2020 Oct 1
TI  - Nanometer precise red blood cell sizing using a cost-effective quantitative dark 
      field imaging system.
PG  - 5950-5966
LID - 10.1364/BOE.405510 [doi]
AB  - Because of the bulk, complexity, calibration requirements, and need for operator 
      training, most current flow-based blood counting devices are not appropriate for 
      field use. Standard imaging methods could be much more compact, inexpensive, and 
      with minimal calibration requirements. However, due to the diffraction limit, 
      imaging lacks the nanometer precision required to measure red blood cell volumes. 
      To address this challenge, we utilize Mie scattering, which can measure 
      nanometer-scale morphological information from cells, in a dark-field imaging 
      geometry. The approach consists of a custom-built dark-field scattering 
      microscope with symmetrically oblique illumination at a precisely defined angle 
      to record wide-field images of diluted and sphered blood samples. Scattering 
      intensities of each cell under three wavelengths are obtained by segmenting 
      images via digital image processing. These scattering intensities are then used 
      to determine size and hemoglobin information via Mie theory and machine learning. 
      Validation on 90 clinical blood samples confirmed the ability to obtain mean 
      corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and 
      red cell distribution width (RDW) with high accuracy. Simulations based on 
      historical data suggest that an instrument with the accuracy achieved in this 
      study could be used for widespread anemia screening.
CI  - (c) 2020 Optical Society of America under the terms of the OSA Open Access 
      Publishing Agreement.
FAU - Chen, Xiaoya
AU  - Chen X
AD  - Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education 
      Institutes, Department of Precision Machinery and Precision Instrumentation, 
      University of Science and Technology of China, Hefei, Anhui, China.
FAU - Luo, Peng
AU  - Luo P
AD  - Department of Clinical Laboratory, The First Affiliated Hospital of University of 
      Science and Technology of China, Hefei, China.
FAU - Hu, Chuanzhen
AU  - Hu C
AD  - Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education 
      Institutes, Department of Precision Machinery and Precision Instrumentation, 
      University of Science and Technology of China, Hefei, Anhui, China.
FAU - Yan, Shaojie
AU  - Yan S
AD  - Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education 
      Institutes, Department of Precision Machinery and Precision Instrumentation, 
      University of Science and Technology of China, Hefei, Anhui, China.
FAU - Lu, Dapeng
AU  - Lu D
AD  - Department of Clinical Laboratory, The First Affiliated Hospital of University of 
      Science and Technology of China, Hefei, China.
FAU - Li, Yaning
AU  - Li Y
AD  - Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education 
      Institutes, Department of Precision Machinery and Precision Instrumentation, 
      University of Science and Technology of China, Hefei, Anhui, China.
FAU - Chu, Kaiqin
AU  - Chu K
AD  - Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education 
      Institutes, Department of Precision Machinery and Precision Instrumentation, 
      University of Science and Technology of China, Hefei, Anhui, China.
AD  - Hefei National Laboratory for Physical Sciences at the Microscale, University of 
      Science and Technology of China, Hefei, Anhui, China.
FAU - Smith, Zachary J
AU  - Smith ZJ
AUID- ORCID: 0000-0002-7946-7863
AD  - Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education 
      Institutes, Department of Precision Machinery and Precision Instrumentation, 
      University of Science and Technology of China, Hefei, Anhui, China.
LA  - eng
PT  - Journal Article
DEP - 20200928
PL  - United States
TA  - Biomed Opt Express
JT  - Biomedical optics express
JID - 101540630
PMC - PMC7587267
COIS- ZJS in a named inventor on US patent 10024858B2. The other authors declare no 
      conflicts of interest.
EDAT- 2020/11/06 06:00
MHDA- 2020/11/06 06:01
PMCR- 2020/10/01
CRDT- 2020/11/05 06:08
PHST- 2020/08/14 00:00 [received]
PHST- 2020/09/16 00:00 [revised]
PHST- 2020/09/18 00:00 [accepted]
PHST- 2020/11/05 06:08 [entrez]
PHST- 2020/11/06 06:00 [pubmed]
PHST- 2020/11/06 06:01 [medline]
PHST- 2020/10/01 00:00 [pmc-release]
AID - 405510 [pii]
AID - 10.1364/BOE.405510 [doi]
PST - epublish
SO  - Biomed Opt Express. 2020 Sep 28;11(10):5950-5966. doi: 10.1364/BOE.405510. 
      eCollection 2020 Oct 1.