PMID- 33150616
OWN - NLM
STAT- MEDLINE
DCOM- 20210223
LR  - 20210223
IS  - 1600-0714 (Electronic)
IS  - 0904-2512 (Linking)
VI  - 50
IP  - 1
DP  - 2021 Jan
TI  - Employing immunohistochemical staining to labial minor salivary gland biopsies 
      from patients with Sjogren's syndrome increases diagnostic certainty.
PG  - 98-102
LID - 10.1111/jop.13119 [doi]
AB  - BACKGROUND: Sjogren's syndrome (SjS) is an autoimmune disease characterized 
      clinically by dry eyes and dry mouth, and histopathologically by lymphocytic 
      infiltrates in the salivary glands. Labial minor salivary gland biopsy (MSGB) is 
      a major diagnostic test for SjS, deemed positive by a focus score of >/=1, meaning 
      that >/=50 lymphocytes were found in 4 mm(2) tissue on hematoxylin and eosin 
      (H&E)-stained slides. The diagnosis can be challenging, and the above diagnostic 
      criteria has low and variable sensitivity. METHODS: We performed a retrospective 
      study on MSGBs done for possible SjS. We compared the percent of MSGBs which met 
      the histologic criteria by H&E stain alone and that with the addition of CD45, 
      CD3, and CD20 immunohistochemical (IHC) staining for these patients. A total of 
      45 cases with complete data were analyzed. RESULTS: Thirty-five of the 45 
      patients had the diagnosis of Sjogren's syndrome (SjS+) based on ACR criteria. 
      However, based on H&E staining alone, only 22/35 cases (63%) met the histologic 
      criteria. After adding IHC staining with CD45, CD3, and CD20 to MSGBs of 
      SjS + patients, 29/35 (83%) cases met the histological criteria for SjS. All 
      MSGBs from patients without SjS had no significant lymphocyte infiltrate on 
      either H&E or IHC stains. CONCLUSIONS: Immunohistochemical better identifies 
      lymphocytic infiltrates in MSGB and increases diagnostic certainty. Due to high 
      cost, their use should be restricted to cases where there is high clinical 
      suspicion of SjS and negative H&E evaluation alone, or if the diagnosis is 
      uncertain.
CI  - (c) 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Trivedi, Apoorva
AU  - Trivedi A
AUID- ORCID: 0000-0002-3884-9785
AD  - Department of Dermatology, University of Massachusetts Medical School, UMass 
      Memorial Medical Center, Worcester, MA, USA.
FAU - Cornejo, Kristine M
AU  - Cornejo KM
AD  - Department of Pathology, Massachusetts General Hospital, Boston, MA, USA.
FAU - O'Donnell, Patrick
AU  - O'Donnell P
AD  - Department of Pathology, University of Massachusetts Medical School, UMass 
      Memorial Medical Center, Worcester, MA, USA.
FAU - Dresser, Karen
AU  - Dresser K
AD  - Department of Pathology, University of Massachusetts Medical School, UMass 
      Memorial Medical Center, Worcester, MA, USA.
FAU - Deng, April
AU  - Deng A
AD  - Department of Pathology, University of Massachusetts Medical School, UMass 
      Memorial Medical Center, Worcester, MA, USA.
LA  - eng
PT  - Journal Article
DEP - 20201127
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
SB  - IM
MH  - Biopsy
MH  - Humans
MH  - Retrospective Studies
MH  - *Salivary Glands, Minor
MH  - *Sjogren's Syndrome/diagnosis
MH  - Staining and Labeling
OTO - NOTNLM
OT  - Sjogren's syndrome
OT  - immunohistochemistry stain
OT  - labial biopsy
OT  - minor salivary gland biopsy
EDAT- 2020/11/06 06:00
MHDA- 2021/02/24 06:00
CRDT- 2020/11/05 06:11
PHST- 2020/05/31 00:00 [received]
PHST- 2020/09/27 00:00 [revised]
PHST- 2020/10/07 00:00 [accepted]
PHST- 2020/11/06 06:00 [pubmed]
PHST- 2021/02/24 06:00 [medline]
PHST- 2020/11/05 06:11 [entrez]
AID - 10.1111/jop.13119 [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2021 Jan;50(1):98-102. doi: 10.1111/jop.13119. Epub 2020 Nov 
      27.