PMID- 33154212
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220418
IS  - 0970-2113 (Print)
IS  - 0974-598X (Electronic)
IS  - 0970-2113 (Linking)
VI  - 37
IP  - 6
DP  - 2020 Nov-Dec
TI  - The diagnostic role of microRNA 21 in patients with nonsmall cell lung cancer: An 
      exploratory study.
PG  - 501-505
LID - 10.4103/lungindia.lungindia_100_20 [doi]
AB  - BACKGROUND: Although histopathological examination of the biopsy specimen is the 
      gold standard for the diagnosis of non small cell lung cancer (NSCLC), a 
      blood-based noninvasive test (liquid biopsy) may prove to be helpful in patients 
      with repeatedly negative biopsy or for response assessment following neoadjuvant 
      therapy. The present study was conducted to explore the diagnostic value of 
      circulating serum microRNA (miRNA) 21 in patients with NSCLC. METHODS: This 
      case-control analytical study was carried out in a tertiary care teaching 
      hospital in Northern India. The study consisted of 30 cases of biopsy-proven 
      NSCLC and 30 controls. Serum miRNA-21 expression levels were estimated by 
      extracting total RNA from the serum sample, reverse transcribing it to cDNA and 
      quantified in relation to U6 reference miRNA. RESULTS: A total of 30 patients 
      with NSCLC and 30 controls were included in the study. The subjects were 
      comparable in two groups with reference to age, gender, and smoking. Pathological 
      types were adenocarcinoma in 19 (63.3%) and squamous cell carcinoma in 11 (36.6%) 
      patients. Majority of the patients had advanced disease-AJCC stage III in 15 
      patients and AJCC Stage IV in 13 patients; two patients had stage II disease. 
      There was a significant upregulation of serum miRNA 21 gene expression in the 
      patients with lung cancer compared to controls (median fold change, 3.39 vs. 
      -2.81, P = 0.00). A fourfold change in serum miRNA 21 is significantly associated 
      with the diagnosis of NSCLC with a high specificity of 97% and area under curve 
      of 0.84 (95% confidence interval of 0.74-0.94). CONCLUSION: Estimation of serum 
      miRNA 21 expression has potential to be used as liquid biopsy for the diagnosis 
      of NSCLC. Further studies with large sample sizes are warranted to confirm the 
      diagnostic accuracy of serum miRNA 21 expression.
FAU - Rai, Shreyash
AU  - Rai S
AD  - Department of Surgery, University College of Medical Sciences and Guru Teg 
      Bahadur Hospital, University of Delhi, India.
FAU - Garg, Pankaj Kumar
AU  - Garg PK
AD  - Department of Surgery, University College of Medical Sciences and Guru Teg 
      Bahadur Hospital, University of Delhi; Department of Surgical Oncology, All India 
      Institute of Medical Sciences, Rishikesh, Uttarakhand, India.
FAU - Bhatt, Shuchi
AU  - Bhatt S
AD  - Department of Radiodiagnosis, University College of Medical Sciences and Guru Teg 
      Bahadur Hospital, University of Delhi, India.
FAU - Latha, Thammineni Krishna
AU  - Latha TK
AD  - Department of Biochemistry, University College of Medical Sciences and Guru Teg 
      Bahadur Hospital, University of Delhi, India.
FAU - Verma, Amit Kumar
AU  - Verma AK
AD  - Department of Medicine, University College of Medical Sciences and Guru Teg 
      Bahadur Hospital, University of Delhi, India.
FAU - Banerjee, Basu Dev
AU  - Banerjee BD
AD  - Department of Biochemistry, University College of Medical Sciences and Guru Teg 
      Bahadur Hospital, University of Delhi, India.
FAU - Singh, Mahendra Pal
AU  - Singh MP
AD  - Department of Surgical Oncology, All India Institute of Medical Sciences, 
      Rishikesh, Uttarakhand, India.
LA  - eng
PT  - Journal Article
PL  - India
TA  - Lung India
JT  - Lung India : official organ of Indian Chest Society
JID - 8405380
PMC - PMC7879878
OTO - NOTNLM
OT  - Diagnosis
OT  - liquid biopsy
OT  - lung neoplasms
OT  - microRNAs
COIS- None
EDAT- 2020/11/07 06:00
MHDA- 2020/11/07 06:01
PMCR- 2020/11/01
CRDT- 2020/11/06 05:52
PHST- 2020/11/06 05:52 [entrez]
PHST- 2020/11/07 06:00 [pubmed]
PHST- 2020/11/07 06:01 [medline]
PHST- 2020/11/01 00:00 [pmc-release]
AID - LungIndia_2020_37_6_501_299649 [pii]
AID - LI-37-501 [pii]
AID - 10.4103/lungindia.lungindia_100_20 [doi]
PST - ppublish
SO  - Lung India. 2020 Nov-Dec;37(6):501-505. doi: 10.4103/lungindia.lungindia_100_20.