PMID- 33154401
OWN - NLM
STAT- MEDLINE
DCOM- 20210309
LR  - 20240330
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Nov 5
TI  - Investigations on spreading of PRRSV among swine herds by improved minimum 
      spanning network analysis.
PG  - 19217
LID - 10.1038/s41598-020-75516-5 [doi]
LID - 19217
AB  - In Hungary, the economic losses caused by porcine reproductive and respiratory 
      syndrome virus (PRRSV) led to the launching of a national PRRSV Eradication 
      Program. An important element of the program was investigating the spread of 
      PRRSV among swine herds and the possible ways of introduction by sequencing of 
      the open reading frame 5 (ORF5) gene. However, the classical phylogenetic tree 
      presentation cannot explain several genetic relationships clearly, while more 
      precise visualization can be represented by network tree diagram. In this paper, 
      we describe a practical and easy-to-follow enriched minimum spanning similarity 
      network application for improved representation of phylogenetic relations among 
      viral strains. This method eliminated the necessity of applying a predefined, 
      arbitrary cut-off or computationally extensive algorithms. The network-based 
      visualization allowed processing and visualizing large amount of data equally for 
      the laboratory, private and official veterinarians, and helped identify the 
      potential connections between different viral sequences that support data-driven 
      decisions in the eradication program. By applying network analysis, previously 
      unknown epidemiological connections between infected herds were identified, and 
      virus spreading was analyzed within short period of time. In our study, we 
      successfully built and applied network analysis tools in the course of the 
      Hungarian PRRSV Eradication Program.
FAU - Szabo, Peter Marton
AU  - Szabo PM
AD  - Hungarian Academy of Sciences and Semmelweis University, Szigony u. 43., 
      Budapest, 1083, Hungary.
FAU - Szalay, Dora
AU  - Szalay D
AD  - Department of Virology, National Food Chain Safety Office Veterinary Diagnostic 
      Directorate, Tabornok u. 2., Budapest, 1143, Hungary.
FAU - Kecskemeti, Sandor
AU  - Kecskemeti S
AD  - Department of Virology, National Food Chain Safety Office Veterinary Diagnostic 
      Directorate, Tabornok u. 2., Budapest, 1143, Hungary.
FAU - Molnar, Tamas
AU  - Molnar T
AD  - National PRRS Eradication Committee, Keleti Karoly u. 24., Budapest, 1024, 
      Hungary.
FAU - Szabo, Istvan
AU  - Szabo I
AD  - National PRRS Eradication Committee, Keleti Karoly u. 24., Budapest, 1024, 
      Hungary.
FAU - Balint, Adam
AU  - Balint A
AD  - Department of Virology, National Food Chain Safety Office Veterinary Diagnostic 
      Directorate, Tabornok u. 2., Budapest, 1143, Hungary. balintad@nebih.gov.hu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201105
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Gene Regulatory Networks
MH  - Genotype
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/*transmission/virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - Swine
MH  - Viral Envelope Proteins/*genetics
PMC - PMC7645787
COIS- The authors declare no competing interests.
EDAT- 2020/11/07 06:00
MHDA- 2021/03/10 06:00
PMCR- 2020/11/05
CRDT- 2020/11/06 05:54
PHST- 2019/09/12 00:00 [received]
PHST- 2020/09/07 00:00 [accepted]
PHST- 2020/11/06 05:54 [entrez]
PHST- 2020/11/07 06:00 [pubmed]
PHST- 2021/03/10 06:00 [medline]
PHST- 2020/11/05 00:00 [pmc-release]
AID - 10.1038/s41598-020-75516-5 [pii]
AID - 75516 [pii]
AID - 10.1038/s41598-020-75516-5 [doi]
PST - epublish
SO  - Sci Rep. 2020 Nov 5;10(1):19217. doi: 10.1038/s41598-020-75516-5.