PMID- 33154752
OWN - NLM
STAT- MEDLINE
DCOM- 20210615
LR  - 20231112
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 11
DP  - 2020
TI  - Increased Hydrostatic Pressure Promotes Primary M1 Reaction and Secondary M2 
      Polarization in Macrophages.
PG  - 573955
LID - 10.3389/fimmu.2020.573955 [doi]
LID - 573955
AB  - Patients with chronic anterior uveitis are at particularly high risk of 
      developing secondary glaucoma when corticosteroids [e.g., dexamethasone (Dex)] 
      are used or when inflammatory activity has regressed. Macrophage migration into 
      the eye increases when secondary glaucoma develops and may play an important role 
      in the development of secondary glaucoma. Our aim was to evaluate in vitro if 
      increased hydrostatic pressure and corticosteroids could induce changes in 
      macrophages phenotype. By using a pressure chamber cell culture system, we 
      assessed the effect of increased hydrostatic pressure (HP), inflammation, and 
      immunosuppression (Dex) on the M1/M2 phenotype of macrophages. Bone 
      marrow-derived macrophages (BMDMs) were stimulated with medium, 
      lipopolysaccharide (LPS, 100 ng/ml), Dex (200 ng/ml), or LPS + Dex and incubated 
      with different HP (0, 20, or 60 mmHg) for 2 or 7 days. The numbers of 
      CD86+/CD206- (M1 phenotype), CD86-/CD206+ (M2 phenotype), CD86+/CD206+ 
      (intermediate phenotype), F4/80+/TNF-alpha+, and F4/80+/IL-10+ macrophages were 
      determined by flow cytometry. TNF-alpha and IL-10 levels in cell culture supernatants 
      were quantified by ELISA. TNF-alpha, IL-10, fibronectin, and collagen IV expression 
      in BMDMs were detected by immunofluorescence microscopy. Higher HP polarizes 
      macrophages primarily to an M1 phenotype (LPS, 60 vs. 0 mmHg, d2: p = 0.0034) 
      with less extra cellular matrix (ECM) production and secondary to an M2 phenotype 
      (medium, 60 vs. 0 mmHg, d7: p = 0.0089) (medium, 60 vs. 20 mmHg, d7: p = 0.0433) 
      with enhanced ECM production. Dex induces an M2 phenotype (Dex, medium vs. Dex, 
      d2: p < 0.0001; d7: p < 0.0001) with more ECM production. Higher HP further 
      increased M2 polarization of Dex-treated macrophages (Dex, 60 vs. 0 mmHg, d2: p = 
      0.0417; d7: p = 0.0454). These changes in the M1/M2 phenotype by high HP or Dex 
      treatment may play a role in the pathogenesis of secondary uveitic glaucoma- or 
      glucocorticoid (GC)-induced glaucoma.
CI  - Copyright (c) 2020 Wang, Kasper, Laffer, Meyer zu Horste, Wasmuth, Busch, 
      Jalilvand, Thanos, Heiligenhaus, Bauer and Heinz.
FAU - Wang, Bo
AU  - Wang B
AD  - Ophthalmology and Ophtha-Lab at St. Franziskus Hospital, Munster, Germany.
FAU - Kasper, Maren
AU  - Kasper M
AD  - Ophthalmology and Ophtha-Lab at St. Franziskus Hospital, Munster, Germany.
FAU - Laffer, Bjorn
AU  - Laffer B
AD  - Ophthalmology and Ophtha-Lab at St. Franziskus Hospital, Munster, Germany.
FAU - Meyer Zu Horste, Gerd
AU  - Meyer Zu Horste G
AD  - Institution of Neurology and Institution for Translational Neurology, 
      Universitatsklinikum Munster, Munster, Germany.
FAU - Wasmuth, Susanne
AU  - Wasmuth S
AD  - Ophthalmology and Ophtha-Lab at St. Franziskus Hospital, Munster, Germany.
FAU - Busch, Martin
AU  - Busch M
AD  - Ophthalmology and Ophtha-Lab at St. Franziskus Hospital, Munster, Germany.
FAU - Jalilvand, Tida Viola
AU  - Jalilvand TV
AD  - Ophthalmology and Ophtha-Lab at St. Franziskus Hospital, Munster, Germany.
FAU - Thanos, Solon
AU  - Thanos S
AD  - Institution of Experimental Ophthalmology, Westfalische Wilhelms-Universitat, 
      Munster, Germany.
FAU - Heiligenhaus, Arnd
AU  - Heiligenhaus A
AD  - Ophthalmology and Ophtha-Lab at St. Franziskus Hospital, Munster, Germany.
AD  - Ophthalmology, University of Duisburg-Essen, Essen, Germany.
FAU - Bauer, Dirk
AU  - Bauer D
AD  - Ophthalmology and Ophtha-Lab at St. Franziskus Hospital, Munster, Germany.
FAU - Heinz, Carsten
AU  - Heinz C
AD  - Ophthalmology and Ophtha-Lab at St. Franziskus Hospital, Munster, Germany.
AD  - Ophthalmology, University of Duisburg-Essen, Essen, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201014
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Collagen Type IV)
RN  - 0 (Fibronectins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Collagen Type IV/metabolism
MH  - Dexamethasone/pharmacology
MH  - Extracellular Matrix/metabolism
MH  - Fibronectins/metabolism
MH  - Glaucoma/etiology/immunology
MH  - Hydrostatic Pressure/*adverse effects
MH  - Inflammation
MH  - Interleukin-10/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages/drug effects/*immunology/metabolism
MH  - Mice
MH  - Phenotype
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC7591771
OTO - NOTNLM
OT  - ECM-extracellular matrix
OT  - M1/M2 balance
OT  - dexamethasone (DEX)
OT  - increased hydrostatic pressure
OT  - macrophage
OT  - secondary glaucoma
EDAT- 2020/11/07 06:00
MHDA- 2021/06/16 06:00
PMCR- 2020/01/01
CRDT- 2020/11/06 06:00
PHST- 2020/06/18 00:00 [received]
PHST- 2020/08/26 00:00 [accepted]
PHST- 2020/11/06 06:00 [entrez]
PHST- 2020/11/07 06:00 [pubmed]
PHST- 2021/06/16 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2020.573955 [doi]
PST - epublish
SO  - Front Immunol. 2020 Oct 14;11:573955. doi: 10.3389/fimmu.2020.573955. eCollection 
      2020.