PMID- 33155245
OWN - NLM
STAT- MEDLINE
DCOM- 20210629
LR  - 20210629
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 24
IP  - 20
DP  - 2020 Oct
TI  - The influence of oncolytic reovirus on the biological activities of umbilical 
      cord-derived mesenchymal stem cells.
PG  - 10839-10849
LID - 23446 [pii]
LID - 10.26355/eurrev_202010_23446 [doi]
AB  - OBJECTIVE: To investigate the influence of oncolytic reovirus on the biological 
      activities of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) as a 
      novel virotherapy strategy. MATERIALS AND METHODS: The Cell Counting Kit-8 assay 
      was used to detect the viability of hUC-MSCs infected with different 
      multiplicities of infection (MOIs) of reoviruses. The biological activities 
      (proliferation, marker expression, multipotency, and migration) of hUC-MSCs were 
      verified by assaying osteogenic and adipogenic differentiation potential, flow 
      cytometry, and electrical cell-substrate impedance sensing, respectively. 
      RESULTS: The viability of hUC-MSCs slightly decreased by infection with low 
      titers of reoviruses. A MOI of 1 had no effect on the viability of hUC-MSCs 
      within 96 h. The biological activities (proliferation, marker expression, 
      multipotency, and migration) of hUC-MSCs were not affected by reovirus infection 
      at a MOI of 1. CONCLUSIONS: Reovirus at a MOI of 1 had no effect on the 
      biological activities of hUC-MSCs.
FAU - Wang, X-Y
AU  - Wang XY
AD  - Stem Cell and Tissue Engineering Research Center, Guizhou Medical University, 
      Guiyang, China. xingzhao@gmc.edu.cn.
FAU - Yang, Y-C
AU  - Yang YC
FAU - Wang, N-X
AU  - Wang NX
FAU - Xu, J-W
AU  - Xu JW
FAU - Zhou, Y-H
AU  - Zhou YH
FAU - Wu, X-J
AU  - Wu XJ
FAU - He, Z-X
AU  - He ZX
FAU - Zhao, X
AU  - Zhao X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Mesenchymal Stem Cells/*metabolism/virology
MH  - Mice
MH  - Reoviridae/isolation & purification/*metabolism
MH  - Umbilical Cord/*metabolism/virology
EDAT- 2020/11/07 06:00
MHDA- 2021/06/30 06:00
CRDT- 2020/11/06 06:02
PHST- 2020/11/06 06:02 [entrez]
PHST- 2020/11/07 06:00 [pubmed]
PHST- 2021/06/30 06:00 [medline]
AID - 23446 [pii]
AID - 10.26355/eurrev_202010_23446 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2020 Oct;24(20):10839-10849. doi: 
      10.26355/eurrev_202010_23446.