PMID- 33156476
OWN - NLM
STAT- MEDLINE
DCOM- 20211006
LR  - 20211006
IS  - 1435-604X (Electronic)
IS  - 0268-8921 (Linking)
VI  - 36
IP  - 7
DP  - 2021 Sep
TI  - Effect of photobiomodulation therapy on the proliferation phase and wound healing 
      in rats fed with an experimental hypoproteic diet.
PG  - 1427-1435
LID - 10.1007/s10103-020-03181-1 [doi]
AB  - Photobiomodulation therapy (PBMT) has been indicated for enforcement on healing 
      skin wounds. This study evaluated the effects of PBMT on the healing of skin 
      wounds during the proliferation phase in rats with a hypoproteic diet. Rats were 
      randomized to one of the following groups (n = 10 per group): (i) injured 
      normoproteic (25% protein) not subjected to PBMT; (ii) injured normoproteic who 
      received PBMT; (iii) injured hypoproteic (8% protein) not subjected to PBMT; and 
      (iv) injured hypoproteic who received PBMT. Rats were submitted to skin wounds 
      and then treated with PBMT (low-level laser therapy: 660 nm, 50 mW, 1.07 W/cm(2), 
      0.028 cm(2), 72 J/cm(2), 2 J). Analyses were performed at 7 and 14 days of 
      follow-up: semi-quantitative histopathologic analysis, collagen type I and III 
      expressions, immunohistochemical marking for matrix metalloproteinases-3 (MMP-3) 
      and (matrix metalloproteinases-9) MMP-9, and mechanical resistance test. There 
      were significant differences between the normoproteic groups and their respective 
      treated groups (p < 0.05), as well as to treated and untreated hypoproteic groups 
      in histopathologic analysis semi-quantitatively and immunohistochemistry for 
      MMP-3 and 9, in which PBMT was able to decrease immunostaining. Moreover, there 
      was a decrease in collagen deposition with the statistical difference (p < 0.05) 
      for both collagen types III and I. In conclusion, PBMT application was proved 
      effective in the treatment of cutaneous wounds in rats submitted to a hypoproteic 
      diet. These alterations were more salient in the proliferation stage with the 
      reduction of metalloproteinases providing better mechanical resistance of the 
      injured area in the remodeling phase with an intensification of type I collagen.
CI  - (c) 2020. Springer-Verlag London Ltd., part of Springer Nature.
FAU - Amadio, Eliane Martins
AU  - Amadio EM
AD  - Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho 
      (UNINOVE), Sao Paulo, SP, Brazil.
FAU - Marcos, Rodrigo Labat
AU  - Marcos RL
AD  - Nine of July University, Postgraduate Program in Applied Biophotonics in Health 
      Sciences, Nove de Julho (UNINOVE), Sao Paulo, SP, Brazil.
FAU - Serra, Andrey Jorge
AU  - Serra AJ
AUID- ORCID: 0000-0002-5407-8183
AD  - Nine of July University, Postgraduate Program in Applied Biophotonics in Health 
      Sciences, Nove de Julho (UNINOVE), Sao Paulo, SP, Brazil. andreyserra@gmail.com.
AD  - Postgraduate Program in Cardiology, Federal University of Sao Paulo, Rua Pedro de 
      Toledo 781, Sao Paulo, SP, Brazil. andreyserra@gmail.com.
FAU - Dos Santos, Solange Almeida
AU  - Dos Santos SA
AD  - Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho 
      (UNINOVE), Sao Paulo, SP, Brazil.
FAU - Caires, Jheniphe Rocha
AU  - Caires JR
AD  - Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho 
      (UNINOVE), Sao Paulo, SP, Brazil.
FAU - Fernandes, Guilherme Henrique Cardosos
AU  - Fernandes GHC
AD  - Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho 
      (UNINOVE), Sao Paulo, SP, Brazil.
FAU - Leal-Junior, Ernesto Cesar
AU  - Leal-Junior EC
AD  - Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho 
      (UNINOVE), Sao Paulo, SP, Brazil.
FAU - Ferrari, Joao Carlos Correa
AU  - Ferrari JCC
AD  - Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho 
      (UNINOVE), Sao Paulo, SP, Brazil.
FAU - de Tarso Camillo de Carvalho, Paulo
AU  - de Tarso Camillo de Carvalho P
AD  - Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho 
      (UNINOVE), Sao Paulo, SP, Brazil.
AD  - Nine of July University, Postgraduate Program in Applied Biophotonics in Health 
      Sciences, Nove de Julho (UNINOVE), Sao Paulo, SP, Brazil.
LA  - eng
GR  - 2018/26062-6/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/
GR  - 434414/2018-2/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
GR  - 307518/2018-3/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
PT  - Journal Article
DEP - 20201106
PL  - England
TA  - Lasers Med Sci
JT  - Lasers in medical science
JID - 8611515
SB  - IM
MH  - Animals
MH  - Cell Proliferation
MH  - Diet
MH  - *Diet, Protein-Restricted
MH  - *Low-Level Light Therapy
MH  - Rats
MH  - Rats, Wistar
MH  - *Wound Healing
OTO - NOTNLM
OT  - Collagen
OT  - Mechanical resistance
OT  - Photobiomodulation therapy metalloproteinases
OT  - Protein malnutrition
OT  - Wound healing
EDAT- 2020/11/07 06:00
MHDA- 2021/10/07 06:00
CRDT- 2020/11/06 12:14
PHST- 2020/03/16 00:00 [received]
PHST- 2020/11/03 00:00 [accepted]
PHST- 2020/11/07 06:00 [pubmed]
PHST- 2021/10/07 06:00 [medline]
PHST- 2020/11/06 12:14 [entrez]
AID - 10.1007/s10103-020-03181-1 [pii]
AID - 10.1007/s10103-020-03181-1 [doi]
PST - ppublish
SO  - Lasers Med Sci. 2021 Sep;36(7):1427-1435. doi: 10.1007/s10103-020-03181-1. Epub 
      2020 Nov 6.