PMID- 33161235
OWN - NLM
STAT- MEDLINE
DCOM- 20210617
LR  - 20210617
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 193
DP  - 2021 Jan 30
TI  - Development and validation of a novel approach based on ultrasonic-assisted 
      enzymatic extraction and RP-UHPLC for simultaneous determination of astaxanthin 
      isomers and alpha-tocopherol in Haematococcuspluvialis derived supplements.
PG  - 113712
LID - S0731-7085(20)31598-3 [pii]
LID - 10.1016/j.jpba.2020.113712 [doi]
AB  - A rapid, effective andgreenmethod was developed for simultaneous determination of 
      total (free and esterified) astaxanthin (AX) isomers (all-E, 9Z and 13Z) and 
      alpha-tocopherol(AT) in Haematococcuspluvialis derived supplements. The new 
      method employed a highly efficient ultrasonic-assisted enzymatic extraction 
      (UAEE) techniqueto perform deesterificationwith Cholesterol esterase from 
      Pseudomonas fluorescenspermitting the concurrent detection. The subsequent 
      RP-UHPLC method for separating and measuring was performed on a simple C18 column 
      within 10.5 min by using methanol and ammonium acetate as mobile phase with a 
      gradient elution. The proposed method was validated according to international 
      guidelines and itproved to be highly accurate and robust. The optimized UHPLC 
      method allowed easy transfer to HPLC, and allowed rapid analysis of active 
      ingredients profiling in H. derived supplements.To our knowledge, this is the 
      first quantification approach describing the rapid simultaneous analysis of the 
      functional lipophilic substances including AX isomers in H. derived supplements 
      using UAEE technique combined with RP-UHPLC.Moreover, this holistic approachcan 
      be used to identify whether AX products are of natural origin or chemical 
      synthesis, and may find more applications in new forms of H. derived productswith 
      complexbiological matrix for more research on the bioavailability of 
      AXisomersfrom natural source.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Lin, Hong-Lin
AU  - Lin HL
AD  - Fujian Provincial Key Laboratory of Zoonosis Research (Fujian Center for Disease 
      Prevention&Control), Fuzhou, 350001, Fujian, China; Teaching Base of School of 
      Public Health, Fujian Medical University, Fuzhou, 350001, Fujian, China. 
      Electronic address: erian@126.com.
FAU - Ni, Lei
AU  - Ni L
AD  - Fujian Provincial Key Laboratory of Zoonosis Research (Fujian Center for Disease 
      Prevention&Control), Fuzhou, 350001, Fujian, China; Teaching Base of School of 
      Public Health, Fujian Medical University, Fuzhou, 350001, Fujian, China.
FAU - Chen, Hua-Feng
AU  - Chen HF
AD  - Fujian Provincial Key Laboratory of Zoonosis Research (Fujian Center for Disease 
      Prevention&Control), Fuzhou, 350001, Fujian, China.
LA  - eng
PT  - Journal Article
DEP - 20201021
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Xanthophylls)
RN  - 8XPW32PR7I (astaxanthine)
RN  - H4N855PNZ1 (alpha-Tocopherol)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - *Ultrasonics
MH  - Xanthophylls
MH  - *alpha-Tocopherol
OTO - NOTNLM
OT  - 13-cis astaxanthin (PubChem CID: 91827159)
OT  - 9-cis astaxanthin (PubChem CID: 91827091)
OT  - All-Transastaxanthin(PubChem CID: 5281224)
OT  - Alpha-Tocopherol (PubChem CID: 2116)
OT  - Alpha-tocopherol
OT  - Astaxanthinisomers
OT  - Haematococcuspluvialis
OT  - UHPLC
OT  - Ultrasonic-assisted enzymatic extraction
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/11/09 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/11/08 20:30
PHST- 2020/08/07 00:00 [received]
PHST- 2020/10/13 00:00 [revised]
PHST- 2020/10/14 00:00 [accepted]
PHST- 2020/11/09 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/11/08 20:30 [entrez]
AID - S0731-7085(20)31598-3 [pii]
AID - 10.1016/j.jpba.2020.113712 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2021 Jan 30;193:113712. doi: 10.1016/j.jpba.2020.113712. 
      Epub 2020 Oct 21.