PMID- 33163246
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201112
IS  - 1598-2629 (Print)
IS  - 2092-6685 (Electronic)
IS  - 1598-2629 (Linking)
VI  - 20
IP  - 5
DP  - 2020 Oct
TI  - Lactoferrin Induces Tolerogenic Bone Marrow-Derived Dendritic Cells.
PG  - e38
LID - 10.4110/in.2020.20.e38 [doi]
LID - e38
AB  - Dendritic cells (DCs) are professional antigen-presenting cells (APCs) that 
      initiate both T-cell responses and tolerance. Tolerogenic DCs (tDCs) are 
      regulatory DCs that suppress immune responses through the induction of T-cell 
      anergy and Tregs. Because lactoferrin (LF) was demonstrated to induce functional 
      Tregs and has a protective effect against inflammatory bowel disease, we explored 
      the tolerogenic effects of LF on mouse bone marrow-derived DCs (BMDCs). The 
      expression of CD80/86 and MHC class II was diminished in LF-treated BMDCs 
      (LF-BMDCs). LF facilitated BMDCs to suppress proliferation and elevate Foxp3(+) 
      induced Treg (iTreg) differentiation in ovalbumin-specific CD4(+) T-cell culture. 
      Foxp3 expression was further increased by blockade of the B7 molecule using 
      CTLA4-Ig but was diminished by additional CD28 stimulation using anti-CD28 Ab. On 
      the other hand, the levels of arginase-1 and indoleamine 2,3-dioxygenase-1 (known 
      as key T-cell suppressive molecules) were increased in LF-BMDCs. Consistently, 
      the suppressive activity of LF-BMDCs was partially restored by inhibitors of 
      these molecules. Collectively, these results suggest that LF effectively causes 
      DCs to be tolerogenic by both the suppression of T-cell proliferation and 
      enhancement of iTreg differentiation. This tolerogenic effect of LF is due to the 
      reduction of costimulatory molecules and enhancement of suppressive molecules.
CI  - Copyright (c) 2020. The Korean Association of Immunologists.
FAU - Park, Hui-Won
AU  - Park HW
AD  - Department of Molecular Bioscience, School of Biomedical Science, Kangwon 
      National University, Chuncheon, Korea.
FAU - Park, Sun-Hee
AU  - Park SH
AD  - Department of Molecular Bioscience, School of Biomedical Science, Kangwon 
      National University, Chuncheon, Korea.
FAU - Jo, Hyeon-Ju
AU  - Jo HJ
AD  - Department of Molecular Bioscience, School of Biomedical Science, Kangwon 
      National University, Chuncheon, Korea.
FAU - Kim, Tae-Gyu
AU  - Kim TG
AD  - Department of Molecular Bioscience, School of Biomedical Science, Kangwon 
      National University, Chuncheon, Korea.
FAU - Lee, Jeong Hyun
AU  - Lee JH
AUID- ORCID: 0000-0002-2801-3677
AD  - Department of Systems Immunology, School of Biomedical Science, Kangwon National 
      University, Chuncheon, Korea.
FAU - Kang, Seung-Goo
AU  - Kang SG
AUID- ORCID: 0000-0003-3981-1399
AD  - Department of Systems Immunology, School of Biomedical Science, Kangwon National 
      University, Chuncheon, Korea.
FAU - Jang, Young-Saeng
AU  - Jang YS
AUID- ORCID: 0000-0001-9844-2074
AD  - Institute of Bioscience and Biotechnology, Kangwon National University, 
      Chuncheon, Korea.
AD  - Department of Molecular Bioscience, School of Biomedical Science, Kangwon 
      National University, Chuncheon, Korea.
FAU - Kim, Pyeung-Hyeun
AU  - Kim PH
AUID- ORCID: 0000-0002-2808-5822
AD  - Institute of Bioscience and Biotechnology, Kangwon National University, 
      Chuncheon, Korea.
AD  - Department of Molecular Bioscience, School of Biomedical Science, Kangwon 
      National University, Chuncheon, Korea.
LA  - eng
PT  - Journal Article
DEP - 20200729
PL  - Korea (South)
TA  - Immune Netw
JT  - Immune network
JID - 101137270
PMC - PMC7609161
OTO - NOTNLM
OT  - B7 antigens
OT  - Dendritic cells
OT  - Immune tolerance
OT  - Lactoferrin
OT  - Regulatory T cells
OT  - Suppressive factor
COIS- Conflicts of Interest: The authors declare no potential conflicts of interest.
EDAT- 2020/11/10 06:00
MHDA- 2020/11/10 06:01
PMCR- 2020/10/01
CRDT- 2020/11/09 05:32
PHST- 2020/06/16 00:00 [received]
PHST- 2020/07/08 00:00 [revised]
PHST- 2020/07/13 00:00 [accepted]
PHST- 2020/11/09 05:32 [entrez]
PHST- 2020/11/10 06:00 [pubmed]
PHST- 2020/11/10 06:01 [medline]
PHST- 2020/10/01 00:00 [pmc-release]
AID - 2020200503 [pii]
AID - 10.4110/in.2020.20.e38 [doi]
PST - epublish
SO  - Immune Netw. 2020 Jul 29;20(5):e38. doi: 10.4110/in.2020.20.e38. eCollection 2020 
      Oct.