PMID- 33163339
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201112
IS  - 2211-3835 (Print)
IS  - 2211-3843 (Electronic)
IS  - 2211-3835 (Linking)
VI  - 10
IP  - 10
DP  - 2020 Oct
TI  - Rapid identification and isolation of neuraminidase inhibitors from 
      mockstrawberry (Duchesnea indica Andr.) based on ligand fishing combined with 
      HR-ESI-Q-TOF-MS.
PG  - 1846-1855
LID - 10.1016/j.apsb.2020.04.001 [doi]
AB  - Neuraminidase inhibitors (NAIs) are the mainstay antiviral drugs against 
      influenza infection. In this study, a ligand fishing protocol was developed to 
      screen NAIs using neuraminidase immobilized magnetic beads (NA-MB). After 
      verifying the feasibility of NA-MB with an artificial mixture including NA 
      inhibitors and non-inhibitors, the developed ligand fishing protocol was applied 
      to screen NAIs from the crude extracts of Duchesnea indica Andr. Twenty-four NA 
      binding compounds were identified from the normal butanol (n-BuOH) extract of D. 
      indica as potential NAIs by high-performance liquid chromatography coupled with 
      quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS) assisted with 
      Compound Structure Identification (CSI):FingerID, including 12 ellagitannins, 4 
      brevifolin derivatives, 3 ellagic acid derivatives, and 4 flavonoids. Among them, 
      9 compounds were isolated and tested for in vitro NA inhibitory activities 
      against NA from Clostridium perfringens, and from oseltamivir sensitive and 
      resistant influenza A virus strains. The results indicate that compound B23 has 
      the NA inhibitory activities in both the oseltamivir sensitive and resistant 
      viral NA, with half maximal inhibitory concentration (IC(50)) values of 197.9 and 
      125.4 mumol/L, respectively. Moreover, B23 can obviously reduce the replication of 
      oseltamivir sensitive and resistant viruses in Madin-Darby canine kidney (MDCK) 
      cells at the concentrations of 40 and 200 mumol/L.
CI  - (c) 2020 Chinese Pharmaceutical Association and Institute of Materia Medica, 
      Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.
FAU - Luo, Sifan
AU  - Luo S
AD  - Institute of Traditional Chinese Medicine and Natural Products, College of 
      Pharmacy, Jinan University, Guangzhou 510632, China.
AD  - Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, 
      Guangzhou 510632, China.
FAU - Guo, Linbo
AU  - Guo L
AD  - Institute of Traditional Chinese Medicine and Natural Products, College of 
      Pharmacy, Jinan University, Guangzhou 510632, China.
FAU - Sheng, Caimin
AU  - Sheng C
AD  - The State Key Laboratory of Respiratory Disease and the 1st Affiliated Hospital 
      of Guangzhou Medical University, Guangzhou 510663, China.
FAU - Zhao, Yumei
AU  - Zhao Y
AD  - Institute of Traditional Chinese Medicine and Natural Products, College of 
      Pharmacy, Jinan University, Guangzhou 510632, China.
AD  - Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, 
      Guangzhou 510632, China.
FAU - Chen, Ling
AU  - Chen L
AD  - The State Key Laboratory of Respiratory Disease and the 1st Affiliated Hospital 
      of Guangzhou Medical University, Guangzhou 510663, China.
FAU - Li, Chufang
AU  - Li C
AD  - The State Key Laboratory of Respiratory Disease and the 1st Affiliated Hospital 
      of Guangzhou Medical University, Guangzhou 510663, China.
FAU - Jiang, Zhengjin
AU  - Jiang Z
AD  - Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, 
      Guangzhou 510632, China.
AD  - International Cooperative Laboratory of Traditional Chinese Medicine 
      Modernization and Innovative Drug Development of Chinese Ministry of Education 
      (MOE), College of Pharmacy, Jinan University, Guangzhou 510632, China.
FAU - Tian, Haiyan
AU  - Tian H
AD  - Institute of Traditional Chinese Medicine and Natural Products, College of 
      Pharmacy, Jinan University, Guangzhou 510632, China.
AD  - International Cooperative Laboratory of Traditional Chinese Medicine 
      Modernization and Innovative Drug Development of Chinese Ministry of Education 
      (MOE), College of Pharmacy, Jinan University, Guangzhou 510632, China.
LA  - eng
PT  - Journal Article
DEP - 20200418
PL  - Netherlands
TA  - Acta Pharm Sin B
JT  - Acta pharmaceutica Sinica. B
JID - 101600560
PMC - PMC7606179
OTO - NOTNLM
OT  - Duchesnea indica Andr.
OT  - HPLC-Q-TOF-MS
OT  - Influenza A
OT  - Ligand fishing
OT  - Magnetic beads
OT  - Neuraminidase inhibitors
OT  - Virus
EDAT- 2020/11/10 06:00
MHDA- 2020/11/10 06:01
PMCR- 2020/04/18
CRDT- 2020/11/09 05:32
PHST- 2020/02/28 00:00 [received]
PHST- 2020/03/23 00:00 [revised]
PHST- 2020/03/31 00:00 [accepted]
PHST- 2020/11/09 05:32 [entrez]
PHST- 2020/11/10 06:00 [pubmed]
PHST- 2020/11/10 06:01 [medline]
PHST- 2020/04/18 00:00 [pmc-release]
AID - S2211-3835(20)30543-8 [pii]
AID - 10.1016/j.apsb.2020.04.001 [doi]
PST - ppublish
SO  - Acta Pharm Sin B. 2020 Oct;10(10):1846-1855. doi: 10.1016/j.apsb.2020.04.001. 
      Epub 2020 Apr 18.