PMID- 33163760
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201112
IS  - 2470-1343 (Electronic)
IS  - 2470-1343 (Linking)
VI  - 5
IP  - 43
DP  - 2020 Nov 3
TI  - Epigenetic Studies Revealed a Ghost Proteome in PC1/3 KD Macrophages under 
      Antitumoral Resistance Induced by IL-10.
PG  - 27774-27782
LID - 10.1021/acsomega.0c02530 [doi]
AB  - Our previous investigation on macrophages has allowed us to show that the 
      inhibition of the enzyme proprotein convertase (PC1/3) controls the activation of 
      macrophages. We demonstrated that PC1/3 knockdown (KD) in macrophages exhibits an 
      increased secretion of proinflammatory and antitumoral factors. In this 
      biological context, we assessed the presence of histone modifications and the 
      presence and contribution of a "ghost proteome" in these macrophages. We 
      identified a set of alternative proteins (AltProts) that have a key role in the 
      regulation of various signaling pathways. In this study, to further investigate 
      the underlying mechanisms involved in the resistance of PC1/3-KD macrophages to 
      anti-inflammatory stimuli, we have conducted a proteomic system biology study to 
      assess the epigenome variation, focusing on histone modifications. Results from 
      our study have indicated the presence of significant variations in histone 
      modifications along with the identification of 28 AltProts, which can be 
      correlated with antitumoral resistance under IL-10 stimulation. These findings 
      highlight a key role of altered epigenome histone modifications in driving 
      resistance and indicate that like the reference proteins, AltProts can have a 
      major impact in the field of epigenetics and regulation of gene expression, as 
      shown in our results.
FAU - Cardon, Tristan
AU  - Cardon T
AD  - Inserm, CHRU Lille, University Lille, U-1192-Laboratoire Proteomique, Reponse 
      Inflammatoire et Spectrometrie de Masse-PRISM, Lille 59000, France.
FAU - Ozcan, Bilgehan
AU  - Ozcan B
AD  - Inserm, CHRU Lille, University Lille, U-1192-Laboratoire Proteomique, Reponse 
      Inflammatoire et Spectrometrie de Masse-PRISM, Lille 59000, France.
FAU - Aboulouard, Soulaimane
AU  - Aboulouard S
AD  - Inserm, CHRU Lille, University Lille, U-1192-Laboratoire Proteomique, Reponse 
      Inflammatoire et Spectrometrie de Masse-PRISM, Lille 59000, France.
FAU - Kobeissy, Firas
AU  - Kobeissy F
AD  - Department of Psychiatry, McKnight Brain Institute, University of Florida, 
      Gainesville, Florida 32611, United States.
FAU - Duhamel, Marie
AU  - Duhamel M
AD  - Inserm, CHRU Lille, University Lille, U-1192-Laboratoire Proteomique, Reponse 
      Inflammatoire et Spectrometrie de Masse-PRISM, Lille 59000, France.
FAU - Rodet, Franck
AU  - Rodet F
AD  - Inserm, CHRU Lille, University Lille, U-1192-Laboratoire Proteomique, Reponse 
      Inflammatoire et Spectrometrie de Masse-PRISM, Lille 59000, France.
FAU - Fournier, Isabelle
AU  - Fournier I
AD  - Inserm, CHRU Lille, University Lille, U-1192-Laboratoire Proteomique, Reponse 
      Inflammatoire et Spectrometrie de Masse-PRISM, Lille 59000, France.
AD  - Institut Universitaire de France, Paris 75000, France.
FAU - Salzet, Michel
AU  - Salzet M
AD  - Inserm, CHRU Lille, University Lille, U-1192-Laboratoire Proteomique, Reponse 
      Inflammatoire et Spectrometrie de Masse-PRISM, Lille 59000, France.
AD  - Institut Universitaire de France, Paris 75000, France.
LA  - eng
PT  - Journal Article
DEP - 20201001
PL  - United States
TA  - ACS Omega
JT  - ACS omega
JID - 101691658
PMC - PMC7643081
COIS- The authors declare no competing financial interest.
EDAT- 2020/11/10 06:00
MHDA- 2020/11/10 06:01
PMCR- 2020/10/01
CRDT- 2020/11/09 05:34
PHST- 2020/05/29 00:00 [received]
PHST- 2020/09/18 00:00 [accepted]
PHST- 2020/11/09 05:34 [entrez]
PHST- 2020/11/10 06:00 [pubmed]
PHST- 2020/11/10 06:01 [medline]
PHST- 2020/10/01 00:00 [pmc-release]
AID - 10.1021/acsomega.0c02530 [doi]
PST - epublish
SO  - ACS Omega. 2020 Oct 1;5(43):27774-27782. doi: 10.1021/acsomega.0c02530. 
      eCollection 2020 Nov 3.