PMID- 33164057
OWN - NLM
STAT- MEDLINE
DCOM- 20210705
LR  - 20210705
IS  - 1569-9285 (Electronic)
IS  - 1569-9285 (Linking)
VI  - 31
IP  - 6
DP  - 2020 Dec 7
TI  - Annexin V positive microvesicles are elevated and correlate with flow rate in 
      patients receiving veno-arterial extracorporeal membrane oxygenation.
PG  - 884-891
LID - 10.1093/icvts/ivaa198 [doi]
AB  - OBJECTIVES: Veno-arterial extracorporeal membrane oxygenation (VA-ECMO) is used 
      in critically ill patients requiring haemodynamic support. Microvesicles (MV) are 
      released by activated blood cells acting as mediators of intercellular 
      communication. We aimed to determine MV count and composition over time in 
      patients with VA-ECMO and explore what drives MV formation. METHODS: VA-ECMO 
      patients and healthy controls were recruited prospectively, and blood was taken 
      at different time points (day 0, 1, 3 after ECMO placement and after 
      explantation) for MV analysis. RESULTS: Annexin V positive MV were increased in 
      patients (n = 14, mean age = 61.4 +/- 9.0 years, 11 males, 3 females) compared to 
      healthy controls (n = 6, Annexin V positive MV count per millilitre day 1 versus 
      healthy controls: 2.3 x 106 vs 1.3 x 105, P < 0.001). Furthermore, patients had 
      higher proportions of endothelial and leukocyte MV [leukocyte MV day 1 versus 
      healthy controls (%): 32.8 vs 17.5, P = 0.001; endothelial MV day 1 versus 
      healthy controls (%): 10.5 vs 5.5, P = 0.01]. Annexin V positive and leucocyte MV 
      correlated with the flow rate (r = 0.46, P = 0.01). CONCLUSIONS: Patients on 
      VA-ECMO have increased levels of circulating MV and a changed MV composition. Our 
      data support the hypothesis that MV release may be driven by higher flow rate and 
      cellular activation in the extracorporeal circuit leading to poor outcomes in 
      these patients. CLINICAL TRIAL REGISTRATION NUMBER: German Clinical Trials 
      Register-ID: DRKS00011106.
CI  - (c) The Author(s) 2020. Published by Oxford University Press on behalf of the 
      European Association for Cardio-Thoracic Surgery. All rights reserved.
FAU - Siegel, Patrick M
AU  - Siegel PM
AD  - Department of Cardiology and Angiology I, Heart Center Freiburg University, 
      Medical Faculty, University of Freiburg, Freiburg, Germany.
FAU - Hentschel, Dominik
AU  - Hentschel D
AD  - Department of Cardiology and Angiology I, Heart Center Freiburg University, 
      Medical Faculty, University of Freiburg, Freiburg, Germany.
FAU - Bojti, Istvan
AU  - Bojti I
AD  - Department of Cardiology and Angiology I, Heart Center Freiburg University, 
      Medical Faculty, University of Freiburg, Freiburg, Germany.
FAU - Wengenmayer, Tobias
AU  - Wengenmayer T
AD  - Department of Cardiology and Angiology I, Heart Center Freiburg University, 
      Medical Faculty, University of Freiburg, Freiburg, Germany.
FAU - Helbing, Thomas
AU  - Helbing T
AD  - Department of Cardiology and Angiology I, Heart Center Freiburg University, 
      Medical Faculty, University of Freiburg, Freiburg, Germany.
FAU - Moser, Martin
AU  - Moser M
AD  - Department of Cardiology and Angiology I, Heart Center Freiburg University, 
      Medical Faculty, University of Freiburg, Freiburg, Germany.
FAU - Duerschmied, Daniel
AU  - Duerschmied D
AD  - Department of Cardiology and Angiology I, Heart Center Freiburg University, 
      Medical Faculty, University of Freiburg, Freiburg, Germany.
FAU - Trummer, Georg
AU  - Trummer G
AD  - Department of Cardiovascular Surgery, Heart Center Freiburg University, Medical 
      Faculty, University of Freiburg, Freiburg, Germany.
FAU - Bode, Christoph
AU  - Bode C
AD  - Department of Cardiology and Angiology I, Heart Center Freiburg University, 
      Medical Faculty, University of Freiburg, Freiburg, Germany.
FAU - Diehl, Philipp
AU  - Diehl P
AD  - Department of Cardiology and Angiology I, Heart Center Freiburg University, 
      Medical Faculty, University of Freiburg, Freiburg, Germany.
LA  - eng
SI  - DRKS/DRKS00011106
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Interact Cardiovasc Thorac Surg
JT  - Interactive cardiovascular and thoracic surgery
JID - 101158399
RN  - 0 (Annexin A5)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Annexin A5/*blood
MH  - Biomarkers/blood
MH  - Critical Illness/*therapy
MH  - Extracorporeal Membrane Oxygenation/*methods
MH  - Female
MH  - *Health Status
MH  - Hemodynamics/*physiology
MH  - Humans
MH  - Male
MH  - Middle Aged
OTO - NOTNLM
OT  - Critical care
OT  - Microvesicles Extracorporeal life support
OT  - Veno-arterial extracorporeal membrane oxygenation
EDAT- 2020/11/10 06:00
MHDA- 2021/07/06 06:00
CRDT- 2020/11/09 05:36
PHST- 2020/03/25 00:00 [received]
PHST- 2020/07/21 00:00 [revised]
PHST- 2020/08/16 00:00 [accepted]
PHST- 2020/11/10 06:00 [pubmed]
PHST- 2021/07/06 06:00 [medline]
PHST- 2020/11/09 05:36 [entrez]
AID - 5961610 [pii]
AID - 10.1093/icvts/ivaa198 [doi]
PST - ppublish
SO  - Interact Cardiovasc Thorac Surg. 2020 Dec 7;31(6):884-891. doi: 
      10.1093/icvts/ivaa198.