PMID- 33165324
OWN - NLM
STAT- MEDLINE
DCOM- 20201207
LR  - 20230511
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 164
DP  - 2020 Oct 26
TI  - A Modified Two Kidney One Clip Mouse Model of Renin Regulation in Renal Artery 
      Stenosis.
LID - 10.3791/61058 [doi]
AB  - Renal artery stenosis is a common condition in patients with coronary or 
      peripheral vascular disease where the renin angiotensin aldosterone system (RAAS) 
      is overactivated. In this context, there is a narrowing of the renal arteries 
      that stimulate an increase in the expression and release of renin, the 
      rate-limiting protease in RAAS. The resulting rise in renin expression is a known 
      driver of renovascular hypertension, frequently associated with kidney injury and 
      end organ damage. Thus, there is a great interest in developing novel treatments 
      for this condition. The molecular and cellular mechanism of renin control in 
      renal artery stenosis is not fully understood and warrants further investigation. 
      To induce renal artery stenosis in mice, a modified 2 kidney 1 clip (2K1C) 
      Goldblatt mouse model was developed. The right kidney was stenosed in wild type 
      mice and sham operated mice were used as control. After renal artery stenosis, we 
      determined renin expression and kidney injury. Kidneys were harvested, and fresh 
      cortices were used to determine protein and mRNA expression of renin. This animal 
      model is reproducible and can be used to study pathophysiological responses, 
      molecular and cellular pathways involved in renovascular hypertension and kidney 
      injury.
FAU - Saleem, Mohammad
AU  - Saleem M
AD  - Department of Medicine/Clinical Pharmacology Division, Vanderbilt University 
      Medical Center.
FAU - Barturen-Larrea, Pierina
AU  - Barturen-Larrea P
AD  - Department of Medicine/Clinical Pharmacology Division, Vanderbilt University 
      Medical Center.
FAU - Saavedra, Luz
AU  - Saavedra L
AD  - Department of Medicine/Clinical Pharmacology Division, Vanderbilt University 
      Medical Center.
FAU - Gomez, Jose A
AU  - Gomez JA
AD  - Department of Medicine/Clinical Pharmacology Division, Vanderbilt University 
      Medical Center; jose.a.gomez@vumc.org.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Video-Audio Media
DEP - 20201026
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (Lipocalin-2)
RN  - 126469-30-5 (Lcn2 protein, mouse)
RN  - EC 3.4.23.15 (Renin)
SB  - IM
MH  - Acute Kidney Injury/*diagnosis/etiology/metabolism
MH  - Animals
MH  - Blood Pressure
MH  - *Disease Models, Animal
MH  - Female
MH  - Kidney/metabolism/*surgery
MH  - Lipocalin-2/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Renal Artery/*physiopathology
MH  - Renal Artery Obstruction/complications/metabolism/*physiopathology
MH  - Renin/*metabolism
EDAT- 2020/11/10 06:00
MHDA- 2020/12/15 06:00
CRDT- 2020/11/09 14:24
PHST- 2020/11/09 14:24 [entrez]
PHST- 2020/11/10 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
AID - 10.3791/61058 [doi]
PST - epublish
SO  - J Vis Exp. 2020 Oct 26;(164). doi: 10.3791/61058.