PMID- 33166843
OWN - NLM
STAT- MEDLINE
DCOM- 20210715
LR  - 20210715
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 251
DP  - 2020 Dec
TI  - Inhibition of porcine reproductive and respiratory syndrome virus by PKC 
      inhibitor dequalinium chloride in vitro.
PG  - 108913
LID - S0378-1135(20)31051-8 [pii]
LID - 10.1016/j.vetmic.2020.108913 [doi]
AB  - As a severe disease characterized by reproductive failure and respiratory 
      distress, porcine reproductive and respiratory syndrome (PRRS) is one of the most 
      leading threats to the swine industry worldwide. Highly evolving porcine 
      reproductive and respiratory syndrome virus (PRRSV) strains with distinct genetic 
      diversity make the current vaccination strategy much less cost-effective and thus 
      urge alternative protective host directed therapeutic approaches. RACK1-PKC-NF-kappaB 
      signalling axis was suggested as a potential therapeutic target for PRRS control, 
      therefore we tested the inhibitory effect of PKC inhibitor dequalinium chloride 
      (DECA) on the PRRSV infection in vitro. RT-qPCR, western blot, Co-IP and 
      cytopathic effect (CPE) observations revealed that DECA suppressed PRRSV 
      infection and protected Marc-145 cells and porcine alveolar macrophages (PAMs) 
      from severe cytopathic effects, by repressing the PKCalpha expression, the 
      interaction between RACK1 and PKCalpha, and subsequently the NF-kappaB activation. In 
      conclusion, the data presented in this study shed more light on deeper 
      understanding of the molecular pathogenesis upon PRRSV infection and more 
      importantly suggested DECA as a potential promising drug candidate for PRRS 
      control.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Liu, Xiao
AU  - Liu X
AD  - College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming 
      650201, Yunnan, China.
FAU - Gao, Libo
AU  - Gao L
AD  - College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming 
      650201, Yunnan, China.
FAU - Zhao, Qian
AU  - Zhao Q
AD  - Center for Animal Disease Control and Prevention, Chuxiong 675000, Yunnan, China.
FAU - Wang, Xiangmin
AU  - Wang X
AD  - College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming 
      650201, Yunnan, China.
FAU - Yang, Chao
AU  - Yang C
AD  - College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming 
      650201, Yunnan, China.
FAU - Bi, Junlong
AU  - Bi J
AD  - Center for Animal Disease Control and Prevention, Chuxiong 675000, Yunnan, China.
FAU - Yang, Runhuan
AU  - Yang R
AD  - College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming 
      650201, Yunnan, China.
FAU - Jin, Xiuli
AU  - Jin X
AD  - First Affiliated Hospital of China Medical University, Shenyang 110001, China.
FAU - Lan, Rui
AU  - Lan R
AD  - College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming 
      650201, Yunnan, China.
FAU - Cui, Rongjun
AU  - Cui R
AD  - College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming 
      650201, Yunnan, China.
FAU - Wang, Xiaochun
AU  - Wang X
AD  - College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming 
      650201, Yunnan, China.
FAU - Li, Wenying
AU  - Li W
AD  - College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming 
      650201, Yunnan, China.
FAU - Wang, Xuesong
AU  - Wang X
AD  - College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming 
      650201, Yunnan, China.
FAU - Yang, Ying
AU  - Yang Y
AD  - College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming 
      650201, Yunnan, China.
FAU - Yu, Xin
AU  - Yu X
AD  - School of Basic Medicine, Dali University, Dali 671003, Yunnan, China.
FAU - Lin, Yingbo
AU  - Lin Y
AD  - Department of Oncology-Pathology, Karolinska Institute, 17176 Stockholm, Sweden.
FAU - Liu, Jianping
AU  - Liu J
AD  - School of Clinical Medicine, Dali University, Dali 671003, Yunnan, China. 
      Electronic address: jianping.liu@ki.se.
FAU - Yin, Gefen
AU  - Yin G
AD  - College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming 
      650201, Yunnan, China. Electronic address: yingefen0616@163.com.
LA  - eng
PT  - Journal Article
DEP - 20201031
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - E7QC7V26B8 (Dequalinium)
RN  - EC 2.7.11.13 (Protein Kinase C-alpha)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cells, Cultured
MH  - Cytopathogenic Effect, Viral
MH  - Dequalinium/*pharmacology
MH  - Macrophages, Alveolar/drug effects/virology
MH  - Porcine respiratory and reproductive syndrome virus/*drug effects
MH  - Protein Kinase C-alpha/*antagonists & inhibitors
MH  - Signal Transduction
MH  - Swine
MH  - Virus Replication/*drug effects
OTO - NOTNLM
OT  - Dequalinium chloride
OT  - NF-kappaB
OT  - PKCalpha
OT  - Porcine reproductive and respiratory syndrome virus
OT  - RACK1
EDAT- 2020/11/10 06:00
MHDA- 2021/07/16 06:00
CRDT- 2020/11/09 20:17
PHST- 2020/05/21 00:00 [received]
PHST- 2020/10/26 00:00 [accepted]
PHST- 2020/11/10 06:00 [pubmed]
PHST- 2021/07/16 06:00 [medline]
PHST- 2020/11/09 20:17 [entrez]
AID - S0378-1135(20)31051-8 [pii]
AID - 10.1016/j.vetmic.2020.108913 [doi]
PST - ppublish
SO  - Vet Microbiol. 2020 Dec;251:108913. doi: 10.1016/j.vetmic.2020.108913. Epub 2020 
      Oct 31.