PMID- 33166900
OWN - NLM
STAT- MEDLINE
DCOM- 20210617
LR  - 20210730
IS  - 1879-2472 (Electronic)
IS  - 0049-3848 (Linking)
VI  - 197
DP  - 2021 Jan
TI  - Neutrophil extracellular traps exacerbate coagulation and endothelial damage in 
      patients with essential hypertension and hyperhomocysteinemia.
PG  - 36-43
LID - S0049-3848(20)30584-3 [pii]
LID - 10.1016/j.thromres.2020.10.028 [doi]
AB  - Patients with essential hypertension (EH) and hyperhomocysteinemia (HHCY) suffer 
      from more increased thrombotic events than those in EH alone. However, the 
      underlying mechanisms for this effect are not well understood. This study 
      hypothesized that neutrophil extracellular trap (NET) releasing may be triggered 
      by HHCY in patients in EH, thereby predisposing them to a more hypercoagulable 
      state. Using a modified-capture enzyme-linked immunosorbent assay (ELISA) method, 
      we observed that cell-free DNA (CF-DNA) and myeloperoxidase DNA (MPO-DNA) in 
      patients With EH and HHCY were significantly higher. The NET formation was also 
      positively correlated with homocysteine levels, neutrophil-lymphocyte ratio 
      (NLR), and hypercoagulable markers (thrombin-antithrombin complex, D-dimers). 
      Furthermore, neutrophils from patients in EH with HHCY were found to be 
      predisposed to amplified NET release when compared to patients in EH without HHCY 
      or CTR. Coagulation function assays showed that NETs in patients With EH and HHCY 
      resulted in a significantly increased ability to generate thrombin and fibrin 
      than in those in EH without HHCY or CTR. These procoagulant effects of NETs in 
      patients With EH and HHCY were markedly inhibited (approximately 70%) by the 
      cleavage of NETs with DNase I. Isolated NETs from patients With EH and HHCY 
      neutrophils also exerted a strong cytotoxic effect on endothelial cells (ECs), 
      converted them to apoptosis. This study revealed a previously unrecognized 
      association between the hypercoagulable state and neutrophils in patients With EH 
      and HHCY. Therefore, blocking NETs may represent a new therapeutic objective for 
      preventing thrombosis in these patients.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Li, Ji-He
AU  - Li JH
AD  - Department of Cardiology, First Affiliated Hospital of Harbin Medical University, 
      Harbin, 150001, Heilongjiang Province, China.
FAU - Tong, Dong-Xia
AU  - Tong DX
AD  - Department of Oncology, Qingdao Municipal Hospital Group, Qingdao 266000, 
      Shandong Province, China.
FAU - Wang, Ying
AU  - Wang Y
AD  - Department of Cardiology, First Affiliated Hospital of Harbin Medical University, 
      Harbin, 150001, Heilongjiang Province, China.
FAU - Gao, Lei
AU  - Gao L
AD  - Department of Cardiology, First Affiliated Hospital of Harbin Medical University, 
      Harbin, 150001, Heilongjiang Province, China.
FAU - Liu, Yue
AU  - Liu Y
AD  - Department of Cardiology, First Affiliated Hospital of Harbin Medical University, 
      Harbin, 150001, Heilongjiang Province, China.
FAU - Zhang, Xiao-Hui
AU  - Zhang XH
AD  - Department of Cardiology, First Affiliated Hospital of Harbin Medical University, 
      Harbin, 150001, Heilongjiang Province, China.
FAU - Chen, Wen-Jia
AU  - Chen WJ
AD  - Department of Cardiology, First Affiliated Hospital of Harbin Medical University, 
      Harbin, 150001, Heilongjiang Province, China.
FAU - Chi, Jin-Yv
AU  - Chi JY
AD  - Department of Cardiology, First Affiliated Hospital of Harbin Medical University, 
      Harbin, 150001, Heilongjiang Province, China.
FAU - Liu, Na
AU  - Liu N
AD  - Department of Cardiology, First Affiliated Hospital of Harbin Medical University, 
      Harbin, 150001, Heilongjiang Province, China.
FAU - Yang, Kelaier
AU  - Yang K
AD  - Department of Cardiology, First Affiliated Hospital of Harbin Medical University, 
      Harbin, 150001, Heilongjiang Province, China.
FAU - Wang, Shi-Peng
AU  - Wang SP
AD  - Department of Cardiology, Second Affiliated Hospital of Harbin Medical 
      University, Harbin, 150001, Heilongjiang Province, China.
FAU - Xu, Yang
AU  - Xu Y
AD  - Department of Cardiology, First Affiliated Hospital of Harbin Medical University, 
      Harbin, 150001, Heilongjiang Province, China.
FAU - Li, Yang
AU  - Li Y
AD  - Department of Cardiology, First Affiliated Hospital of Harbin Medical University, 
      Harbin, 150001, Heilongjiang Province, China.
FAU - Yin, Xin-Hua
AU  - Yin XH
AD  - Department of Cardiology, First Affiliated Hospital of Harbin Medical University, 
      Harbin, 150001, Heilongjiang Province, China. Electronic address: 
      yinxihua365@163.com.
FAU - Liu, Wen-Xiu
AU  - Liu WX
AD  - Department of Cardiology, First Affiliated Hospital of Harbin Medical University, 
      Harbin, 150001, Heilongjiang Province, China. Electronic address: 
      liuwx65240@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Retracted Publication
DEP - 20201101
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
SB  - IM
RIN - J Thromb Haemost. 2021 Aug;19(8):2096. PMID: 33314547
MH  - Blood Coagulation
MH  - Endothelial Cells
MH  - Essential Hypertension
MH  - *Extracellular Traps
MH  - Humans
MH  - *Hyperhomocysteinemia/complications
MH  - Neutrophils
OTO - NOTNLM
OT  - Endothelial cells
OT  - Essential hypertension
OT  - Hyperhomocysteinemia
OT  - Neutrophil extracellular traps
OT  - Platelets
EDAT- 2020/11/10 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/11/09 20:19
PHST- 2020/06/08 00:00 [received]
PHST- 2020/10/25 00:00 [revised]
PHST- 2020/10/26 00:00 [accepted]
PHST- 2020/11/10 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/11/09 20:19 [entrez]
AID - S0049-3848(20)30584-3 [pii]
AID - 10.1016/j.thromres.2020.10.028 [doi]
PST - ppublish
SO  - Thromb Res. 2021 Jan;197:36-43. doi: 10.1016/j.thromres.2020.10.028. Epub 2020 
      Nov 1.