PMID- 33167095
OWN - NLM
STAT- MEDLINE
DCOM- 20210118
LR  - 20210118
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 36
IP  - 4
DP  - 2020 Jul
TI  - [miR-520a-3p regulates the signaling pathway of cytokine secretion in cervical 
      cancer].
PG  - 340-345
LID - 10.12047/j.cjap.5935.2020.073 [doi]
AB  - OBJECTIVE: To investigate the molecular mechanism of miR-520a-3p regulating the 
      secretion of cytokines in cervical cancer. METHODS: The matching between 
      miR-520a-3p and the NF-kappaB complex subunit RELA was analyzed by TargetScanHuman, 
      and then the luciferase reporting system was used to detect whether miR-520a-3p 
      targeted the NF-kappaB complex subunit RELA. After cervical cancer HELA cells 
      stimulated by LPS, in the overexpression group or the knockout group, miR-520a-3p 
      mimics or inhibitor were mixed with transfection reagent and dripped into HELA 
      cells. The expression levels of colony stimulating factor (GCSF), 
      granulocyte-macrophage colony stimulating factor (GM-CSF), interleukin 2 (IL-2), 
      interleukin 3 (IL-3), interleukin 4 (IL-4), interleukin 5 (IL-5), interleukin 6 
      (IL-6), interleukin 19 (IL-9), interleukin 10 (IL-10), interleukin 12 p40 (IL-12 
      p40), interleukin 12 p70 (IL-12 p70), interleukin 13 (IL-13), interleukin 17 
      (IL-17), interferon gamma (IFN-gamma), monocyte chemoattractant protein-1 (MCP-1), 
      monocyte chemoattractant protein-5 (MCP-5), small inducible cytokine A5 (RANTES), 
      tumor necrosis factor-alpha (TNFalpha) were detected by enzyme-linked immunosorbent 
      assay in both the overexpression group and the knockout group. Each experiment 
      was repeated three times. RESULTS: miR-520a-3p targeted the 3'UTR of RELA. After 
      activation of the NF-kappaB signaling pathway by lipopolysaccharide (LPS), the 
      protein expression levels of cytokines GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, 
      IL-6, IL-9, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-17, IFN-gamma, MCP-1, MCP-5, 
      RANTES, TNFalpha secreted by HELA cells of cervical cancer were increased 
      significantly (P＜0.05). In the overexpression group, the protein expression level 
      of RELA of NF-kappaB complex subunit was decreased, and the protein expression level 
      of the above-mentioned cytokines secreted by HELA cells of cervical cancer were 
      decreased (P＜0.05). In the knockdown group, the protein expression level of RELA, 
      a subunit of NF-kappaB complex, and the protein expression level of the 
      above-mentioned cytokines secreted by HELA cells of cervical cancer were 
      increased (P＜0.05). CONCLUSION: miR-520a-3p inhibits cytokine secretion in HELA 
      cells of cervical cancer by targeting RELA, a key molecule in the NF-kappaB signaling 
      pathway.
FAU - Liu, Hong-Yan
AU  - Liu HY
AD  - Tumor Radiotherapy Center of Central Hospital of Enshi Tujia Miao Autonomous 
      Prefecture, Hubei Province, Enshi 445000.
FAU - Lu, Qi-Hong
AU  - Lu QH
AD  - CentralHospital of Enshi Tujia and Miao Autonomous Prefecture, Hubei Province, 
      Department of Neurology, Enshi 445000.
FAU - Huang, Chu-Ying
AU  - Huang CY
AD  - Department of Oncology, Central Hospital of Enshi Tujia and Miao Autonomous 
      Prefectures, Hubei Province, Enshi 445000, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
RN  - 0 (Cytokines)
RN  - 0 (MicroRNAs)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Cytokines
MH  - Female
MH  - HeLa Cells
MH  - Humans
MH  - *MicroRNAs/genetics
MH  - NF-kappa B/metabolism
MH  - *Signal Transduction
MH  - *Uterine Cervical Neoplasms/genetics
OTO - NOTNLM
OT  - HELA cells of cervical cancer
OT  - NF-kappaB
OT  - cytokines
OT  - miR-520a-3p
EDAT- 2020/11/10 06:00
MHDA- 2021/01/20 06:00
CRDT- 2020/11/09 20:22
PHST- 2020/11/09 20:22 [entrez]
PHST- 2020/11/10 06:00 [pubmed]
PHST- 2021/01/20 06:00 [medline]
AID - 10.12047/j.cjap.5935.2020.073 [doi]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2020 Jul;36(4):340-345. doi: 
      10.12047/j.cjap.5935.2020.073.