PMID- 33168885 OWN - NLM STAT- MEDLINE DCOM- 20210503 LR - 20210503 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 10 IP - 1 DP - 2020 Nov 9 TI - Effect and mechanism of human umbilical cord mesenchymal stem cells in treating allergic rhinitis in mice. PG - 19295 LID - 10.1038/s41598-020-76343-4 [doi] LID - 19295 AB - A model of allergic rhinitis (AR) in BALB/c mice was established and evaluated to provide experimental subjects for further research. Preparation of human umbilical cord mesenchymal stem cells (hUCMSCs), including isolation, expansion culture, passaging, cryopreservation, and preparation of cell suspensions, provided materials for experimental research and clinical treatment. The mouse AR model was established by ovalbumin (OVA) intraperitoneal injection and the nasal stimulation induction method, and the model had a good effect and high repeatability. GFP-labeled hUCMSCs had good effects and were stable cells that could be used for tracking in animals. Transplantation of hUCMSCs by intraperitoneal and tail vein injections had a specific effect on the AR model of mice, and tail vein injection had a better effect. Tracking of hUCMSCs in vivo showed that the three groups of mice had the greatest number of hUCMSCs in the nose at week 2. The mouse AR model was used to evaluate the efficacy of hUCMSC transplantation via multiple methods for AR. The distribution of hUCMSCs in vivo was tracked by detecting green fluorescent protein (GFP), and the treatment mechanism of hUCMSCs was elucidated. This study provides technical methods and a theoretical basis for the clinical application of hUCMSCs. FAU - Kan, Xiao-Li AU - Kan XL AD - Kunming Key Laboratory of Stem Cell and Regenerative Medicine, 920th Hospital of the PLA Joint Logistics Support Force, Kunming, 650032, Yunnan, China. AD - Stem Cell and Immune Cell Biomedical Techniques and Integrated Engineering Laboratory of State and Regions, Kunming, Yunnan, China. AD - Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, Yunnan, China. FAU - Pan, Xing-Hua AU - Pan XH AD - Kunming Key Laboratory of Stem Cell and Regenerative Medicine, 920th Hospital of the PLA Joint Logistics Support Force, Kunming, 650032, Yunnan, China. AD - Stem Cell and Immune Cell Biomedical Techniques and Integrated Engineering Laboratory of State and Regions, Kunming, Yunnan, China. AD - Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, Yunnan, China. FAU - Zhao, Jing AU - Zhao J AD - Kunming Key Laboratory of Stem Cell and Regenerative Medicine, 920th Hospital of the PLA Joint Logistics Support Force, Kunming, 650032, Yunnan, China. AD - Stem Cell and Immune Cell Biomedical Techniques and Integrated Engineering Laboratory of State and Regions, Kunming, Yunnan, China. AD - Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, Yunnan, China. FAU - He, Jie AU - He J AD - Kunming Key Laboratory of Stem Cell and Regenerative Medicine, 920th Hospital of the PLA Joint Logistics Support Force, Kunming, 650032, Yunnan, China. AD - Stem Cell and Immune Cell Biomedical Techniques and Integrated Engineering Laboratory of State and Regions, Kunming, Yunnan, China. AD - Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, Yunnan, China. FAU - Cai, Xue-Min AU - Cai XM AD - Kunming Key Laboratory of Stem Cell and Regenerative Medicine, 920th Hospital of the PLA Joint Logistics Support Force, Kunming, 650032, Yunnan, China. AD - Stem Cell and Immune Cell Biomedical Techniques and Integrated Engineering Laboratory of State and Regions, Kunming, Yunnan, China. AD - Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, Yunnan, China. FAU - Pang, Rong-Qing AU - Pang RQ AD - Kunming Key Laboratory of Stem Cell and Regenerative Medicine, 920th Hospital of the PLA Joint Logistics Support Force, Kunming, 650032, Yunnan, China. AD - Stem Cell and Immune Cell Biomedical Techniques and Integrated Engineering Laboratory of State and Regions, Kunming, Yunnan, China. AD - Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, Yunnan, China. FAU - Zhu, Xiang-Qing AU - Zhu XQ AD - Kunming Key Laboratory of Stem Cell and Regenerative Medicine, 920th Hospital of the PLA Joint Logistics Support Force, Kunming, 650032, Yunnan, China. AD - Stem Cell and Immune Cell Biomedical Techniques and Integrated Engineering Laboratory of State and Regions, Kunming, Yunnan, China. AD - Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, Yunnan, China. FAU - Cao, Xian-Bao AU - Cao XB AD - Department of Otorhinolaryngology, Kunming First People's Hospital, Kunming, Yunnan, China. ynkmcxb@126.com. FAU - Ruan, Guang-Ping AU - Ruan GP AD - Kunming Key Laboratory of Stem Cell and Regenerative Medicine, 920th Hospital of the PLA Joint Logistics Support Force, Kunming, 650032, Yunnan, China. ruangp@126.com. AD - Stem Cell and Immune Cell Biomedical Techniques and Integrated Engineering Laboratory of State and Regions, Kunming, Yunnan, China. ruangp@126.com. AD - Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, Yunnan, China. ruangp@126.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20201109 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 RN - 0 (IL10 protein, mouse) RN - 0 (Il4 protein, mouse) RN - 0 (Interleukin-6) RN - 0 (interleukin-6, mouse) RN - 130068-27-8 (Interleukin-10) RN - 147336-22-9 (Green Fluorescent Proteins) RN - 207137-56-2 (Interleukin-4) RN - 82115-62-6 (Interferon-gamma) SB - IM MH - Animals MH - Behavior, Animal MH - *Cord Blood Stem Cell Transplantation MH - Disease Models, Animal MH - Female MH - Green Fluorescent Proteins/metabolism MH - Humans MH - Interferon-gamma/metabolism MH - Interleukin-10/metabolism MH - Interleukin-4/metabolism MH - Interleukin-6/metabolism MH - *Mesenchymal Stem Cell Transplantation MH - Mesenchymal Stem Cells/*cytology MH - Mice MH - Mice, Inbred BALB C MH - Polymerase Chain Reaction MH - Rhinitis, Allergic/metabolism/*therapy MH - Umbilical Cord/cytology PMC - PMC7652838 COIS- The authors declare no competing interests. EDAT- 2020/11/11 06:00 MHDA- 2021/05/04 06:00 PMCR- 2020/11/09 CRDT- 2020/11/10 05:42 PHST- 2020/05/28 00:00 [received] PHST- 2020/10/27 00:00 [accepted] PHST- 2020/11/10 05:42 [entrez] PHST- 2020/11/11 06:00 [pubmed] PHST- 2021/05/04 06:00 [medline] PHST- 2020/11/09 00:00 [pmc-release] AID - 10.1038/s41598-020-76343-4 [pii] AID - 76343 [pii] AID - 10.1038/s41598-020-76343-4 [doi] PST - epublish SO - Sci Rep. 2020 Nov 9;10(1):19295. doi: 10.1038/s41598-020-76343-4.