PMID- 33168891
OWN - NLM
STAT- MEDLINE
DCOM- 20210503
LR  - 20240330
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Nov 9
TI  - Caloric restriction modulates the monoaminergic system and metabolic hormones in 
      aged rats.
PG  - 19299
LID - 10.1038/s41598-020-76219-7 [doi]
LID - 19299
AB  - Caloric restriction (CR) can attenuate the general loss of health observed during 
      aging, being one of the mechanisms involved the reduction of hormonal alteration, 
      such as insulin and leptin. This change could also prevent age-specific 
      fluctuations in brain monoamines, although few studies have addressed the effects 
      of CR on peripheral hormones and central neurotransmitters exhaustively. 
      Therefore, the variations in brain monoamine levels and some peripheral hormones 
      were assessed here in adult 4-month old and 24-month old male Wistar rats fed ad 
      libitum (AL) or maintained on a 30% CR diet from four months of age. 
      Noradrenaline (NA), dopamine (DA), serotonin (5-HT) and its metabolites were 
      measured by high-performance liquid chromatography with electrochemical detection 
      (HPLC-ED) in nine brain regions: cerebellum, pons, midbrain, hypothalamus, 
      thalamus, hippocampus, striatum, frontal cortex, and occipital cortex. In 
      addition, the blood plasma levels of hormones like corticosterone, insulin and 
      leptin were also evaluated, as were insulin-like growth factor 1 and other basal 
      metabolic parameters using enzyme-linked immunosorbent assays (ELISAs): 
      cholesterol, glucose, triglycerides, albumin, low-density lipoprotein, calcium 
      and high-density lipoprotein (HDLc). CR was seen to increase the NA levels that 
      are altered by aging in specific brain regions like the striatum, thalamus, 
      cerebellum and hypothalamus, and the DA levels in the striatum, as well as 
      modifying the 5-HT levels in the striatum, hypothalamus, pons and hippocampus. 
      Moreover, the insulin, leptin, calcium and HDLc levels in the blood were restored 
      in old animals maintained on a CR diet. These results suggest that a dietary 
      intervention like CR may have beneficial health effects, recovering some negative 
      effects on peripheral hormones, metabolic parameters and brain monoamine 
      concentrations.
FAU - Portero-Tresserra, Marta
AU  - Portero-Tresserra M
AD  - Departament de Psicobiologia i Metodologia de les Ciencies de la Salut, Institut 
      de Neurociencies, Universitat Autonoma de Barcelona, Barcelona, Spain. 
      marta.portero@uab.cat.
FAU - Rojic-Becker, D
AU  - Rojic-Becker D
AD  - Departament de Psicobiologia i Metodologia de les Ciencies de la Salut, Institut 
      de Neurociencies, Universitat Autonoma de Barcelona, Barcelona, Spain.
FAU - Vega-Carbajal, C
AU  - Vega-Carbajal C
AD  - Departament de Psicobiologia i Metodologia de les Ciencies de la Salut, Institut 
      de Neurociencies, Universitat Autonoma de Barcelona, Barcelona, Spain.
FAU - Guillazo-Blanch, G
AU  - Guillazo-Blanch G
AD  - Departament de Psicobiologia i Metodologia de les Ciencies de la Salut, Institut 
      de Neurociencies, Universitat Autonoma de Barcelona, Barcelona, Spain.
FAU - Vale-Martinez, A
AU  - Vale-Martinez A
AD  - Departament de Psicobiologia i Metodologia de les Ciencies de la Salut, Institut 
      de Neurociencies, Universitat Autonoma de Barcelona, Barcelona, Spain.
FAU - Marti-Nicolovius, M
AU  - Marti-Nicolovius M
AD  - Departament de Psicobiologia i Metodologia de les Ciencies de la Salut, Institut 
      de Neurociencies, Universitat Autonoma de Barcelona, Barcelona, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201109
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Amines)
RN  - 0 (Insulin)
RN  - 0 (Neurotransmitter Agents)
RN  - 333DO1RDJY (Serotonin)
RN  - VTD58H1Z2X (Dopamine)
RN  - W980KJ009P (Corticosterone)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Amines/*metabolism
MH  - Animals
MH  - Brain/*metabolism
MH  - *Caloric Restriction
MH  - Chromatography, High Pressure Liquid
MH  - Corpus Striatum/metabolism
MH  - Corticosterone/metabolism
MH  - Dopamine/*metabolism
MH  - Frontal Lobe/metabolism
MH  - Hippocampus/metabolism
MH  - Hypothalamus/metabolism
MH  - Insulin/metabolism
MH  - Male
MH  - Neostriatum/metabolism
MH  - Neurotransmitter Agents/*metabolism
MH  - Norepinephrine/*metabolism
MH  - Prefrontal Cortex/metabolism
MH  - Rats
MH  - Serotonin/metabolism
PMC - PMC7653031
COIS- The authors declare no competing interests.
EDAT- 2020/11/11 06:00
MHDA- 2021/05/04 06:00
PMCR- 2020/11/09
CRDT- 2020/11/10 05:42
PHST- 2020/01/19 00:00 [received]
PHST- 2020/09/30 00:00 [accepted]
PHST- 2020/11/10 05:42 [entrez]
PHST- 2020/11/11 06:00 [pubmed]
PHST- 2021/05/04 06:00 [medline]
PHST- 2020/11/09 00:00 [pmc-release]
AID - 10.1038/s41598-020-76219-7 [pii]
AID - 76219 [pii]
AID - 10.1038/s41598-020-76219-7 [doi]
PST - epublish
SO  - Sci Rep. 2020 Nov 9;10(1):19299. doi: 10.1038/s41598-020-76219-7.