PMID- 33171116
OWN - NLM
STAT- MEDLINE
DCOM- 20210209
LR  - 20210209
IS  - 1531-5053 (Electronic)
IS  - 0278-2391 (Linking)
VI  - 79
IP  - 2
DP  - 2021 Feb
TI  - Overexpression of HIF-1alpha in Bone Marrow Mesenchymal Stem Cells Promote the 
      Repair of Mandibular Condylar Osteochondral Defect in a Rabbit Model.
PG  - 345.e1-345.e15
LID - S0278-2391(20)31244-1 [pii]
LID - 10.1016/j.joms.2020.10.013 [doi]
AB  - PURPOSE: The self-repair ability of temporomandibular joint (TMJ) cartilage is 
      limited. Hypoxia-inducible factor-1 alpha (HIF-1alpha) may induce stem cells to 
      promote chondrogenic repair. The purpose of this study was to systematically 
      evaluate the effect of HIF-1alpha overexpression in bone marrow mesenchymal stem 
      cells (BMSCs) combined with collagen scaffolds on the repair of TMJ condylar 
      osteochondral defects in a rabbit model. METHODS: Osteochondral defects of 3-mm 
      diameter x 2-mm depth were created at the right side of the mandibular condyle in 
      40 New Zealand white rabbits. The defect sites were treated with simple empty, 
      collagen scaffolds (COL), BMSCs/COL, and HIF-1alpha overexpression BMSCs/COL 
      groups. The histomorphologic features of condylar cartilage were monitored by 
      gross examination, safranin O-fast green staining (Solarbio, Beijing, China), and 
      immunohistochemical staining. The changes in subchondral bone were examined by 
      microcomputed tomography. Immunofluorescence staining was used to trace the 
      transplanted BMSCs in vivo. RESULTS: At 12 weeks postimplantation, histologic 
      staining showed that the osteochondral defects in the simple empty and COL groups 
      were mainly filled with fibrous tissue, whereas the BMSCs/COL and HIF-1alpha 
      overexpression BMSCs/COL groups repaired the defect with fibrocartilage. 
      Furthermore, the cartilage was better organized in the HIF-1alpha overexpression 
      BMSCs/COL group compared with the BMSCs/COL group. Microcomputed tomography 
      showed that osteochondral defects can cause abnormal hyperosteogeny in 
      subchondral bone, and the transplantation of BMSCs, especially HIF-1alpha 
      overexpression BMSCs, may alleviate osteosclerosis. Immunofluorescence staining 
      showed that HIF-1alpha overexpression can promote the survival of transplanted 
      BMSCs. CONCLUSIONS: The transplantation of HIF-1alpha overexpression BMSCs 
      combined with a COL scaffold promotes cartilaginous repair of condylar cartilage 
      and inhibits subchondral bone sclerosis in TMJ condylar osteochondral defect 
      rabbits.
CI  - Copyright (c) 2020 American Association of Oral and Maxillofacial Surgeons. 
      Published by Elsevier Inc. All rights reserved.
FAU - Cheng, Mo-Sha
AU  - Cheng MS
AD  - Doctoral Candidate, Department of Oral and Maxillofacial Surgery, School and 
      Hospital of Stomatology, China Medical University, Liaoning Provincial Key 
      Laboratory of Oral Diseases, Shenyang, Liaoning Province, China.
FAU - Yi, Xin
AU  - Yi X
AD  - Lecturer, Department of Oral Anatomy and Physiology, School and Hospital of 
      Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral 
      Diseases, Shenyang, Liaoning Province, China.
FAU - Zhou, Qing
AU  - Zhou Q
AD  - Doctoral Candidate, Department of Oral and Maxillofacial Surgery, School and 
      Hospital of Stomatology, China Medical University, Liaoning Provincial Key 
      Laboratory of Oral Diseases, Shenyang, Liaoning Province, China. Electronic 
      address: zqforstudent@163.com.
LA  - eng
PT  - Journal Article
DEP - 20201016
PL  - United States
TA  - J Oral Maxillofac Surg
JT  - Journal of oral and maxillofacial surgery : official journal of the American 
      Association of Oral and Maxillofacial Surgeons
JID - 8206428
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - Cartilage
MH  - *Cartilage, Articular/surgery
MH  - China
MH  - Mandibular Condyle/surgery
MH  - *Mesenchymal Stem Cells
MH  - Rabbits
MH  - Tissue Engineering
MH  - Tissue Scaffolds
MH  - X-Ray Microtomography
EDAT- 2020/11/11 06:00
MHDA- 2021/02/10 06:00
CRDT- 2020/11/10 20:09
PHST- 2020/07/09 00:00 [received]
PHST- 2020/09/18 00:00 [revised]
PHST- 2020/10/10 00:00 [accepted]
PHST- 2020/11/11 06:00 [pubmed]
PHST- 2021/02/10 06:00 [medline]
PHST- 2020/11/10 20:09 [entrez]
AID - S0278-2391(20)31244-1 [pii]
AID - 10.1016/j.joms.2020.10.013 [doi]
PST - ppublish
SO  - J Oral Maxillofac Surg. 2021 Feb;79(2):345.e1-345.e15. doi: 
      10.1016/j.joms.2020.10.013. Epub 2020 Oct 16.