PMID- 33172121
OWN - NLM
STAT- MEDLINE
DCOM- 20210726
LR  - 20210726
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 11
IP  - 11
DP  - 2020 Nov 6
TI  - B Chromosomes and Cytogenetic Characteristics of the Common Nase Chondrostoma 
      nasus (Linnaeus, 1758).
LID - 10.3390/genes11111317 [doi]
LID - 1317
AB  - Supernumerary B chromosomes (Bs) are very promising structures, among others, in 
      that they are an additional genomic compartment for evolution. In this study, we 
      tested the presence and frequency of B chromosomes and performed the first 
      cytogenetic examination of the common nase (Chondrostoma nasus). We investigated 
      the individuals from two populations in the Vistula River basin, in Poland, 
      according to the chromosomal distribution of the C-bands and silver nucleolar 
      organizer regions (Ag-NORs), using sequential staining with AgNO(3) and 
      chromomycin A(3) (CMA(3)). Furthermore, we analyzed the chromosomal localization 
      of two rDNA families (45S and 5S rDNA) using fluorescence in situ hybridization 
      (FISH) with rDNA probes. C. nasus individuals showed a standard (A) chromosome 
      set consisting of 2n = 50: 12 metacentric, 32 submetacentric, and 6 acrocentric 
      chromosomes (NF = 94). Fourteen out of the 20 analyzed individuals showed 1-2 
      mitotically unstable submetacentric B chromosomes of different sizes. Six of 
      them, in 14.1% of the analyzed metaphase plates, had a single, medium-sized 
      submetacentric B (Bsm) chromosome (2n = 51) with a heterochromatic block located 
      in its pericentromeric region. The other seven individuals possessed a Bsm (2n = 
      51) in 19.4% of the analyzed metaphase plates, and a second Bsm chromosome (2n = 
      52), the smallest in the set, in 15.5% of metaphase plates, whereas one female 
      was characterized by both Bsm chromosomes (2n = 52) in 14.3% of the analyzed 
      metaphase plates. AgNORs, GC-rich DNA sites, and 28S rDNA hybridization sites 
      were observed in the short arms of two submetacentric chromosome pairs of A set. 
      The constitutive heterochromatin was visible as C bands in the centromeric 
      regions of almost all C. nasus chromosomes and in the pericentromeric region of 
      several chromosome pairs. Two 5S rDNA hybridization sites in the pericentromeric 
      position of the largest acrocentric chromosome pair were observed, whereas two 
      other such sites in co-localization on a smaller pair of NOR chromosomes indicate 
      a species-specific character. The results herein broaden our knowledge in the 
      field of B chromosome distribution and molecular cytogenetics of C. nasus: a 
      freshwater species from the Leuciscidae family.
FAU - Boron, Alicja
AU  - Boron A
AUID- ORCID: 0000-0003-3888-617X
AD  - Department of Zoology, Faculty of Biology and Biotechnology, University of Warmia 
      and Mazury, M. Oczapowskiego St. 5, 10-718 Olsztyn, Poland.
FAU - Grabowska, Anna
AU  - Grabowska A
AUID- ORCID: 0000-0002-2749-5897
AD  - Department of Zoology, Faculty of Biology and Biotechnology, University of Warmia 
      and Mazury, M. Oczapowskiego St. 5, 10-718 Olsztyn, Poland.
FAU - Spoz, Aneta
AU  - Spoz A
AD  - Department of Zoology, Faculty of Biology and Biotechnology, University of Warmia 
      and Mazury, M. Oczapowskiego St. 5, 10-718 Olsztyn, Poland.
FAU - Przybyl, Anna
AU  - Przybyl A
AUID- ORCID: 0000-0001-7480-9302
AD  - Department of Zoology, Faculty of Biology and Biotechnology, University of Warmia 
      and Mazury, M. Oczapowskiego St. 5, 10-718 Olsztyn, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201106
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Animals
MH  - Centromere/genetics
MH  - Chromosome Banding/methods
MH  - Chromosomes, Human, 4-5/*genetics
MH  - Cyprinidae/*genetics
MH  - Cytogenetic Analysis/methods
MH  - DNA, Ribosomal/genetics
MH  - Heterochromatin/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping/methods
MH  - Nucleolus Organizer Region/genetics
MH  - Poland
MH  - Species Specificity
PMC - PMC7694786
OTO - NOTNLM
OT  - B chromosomes
OT  - C-banding
OT  - CMA3
OT  - FISH
OT  - Leuciscidae
OT  - rDNA sequences
COIS- The authors declare no conflict of interest.
EDAT- 2020/11/12 06:00
MHDA- 2021/07/27 06:00
PMCR- 2020/11/01
CRDT- 2020/11/11 01:03
PHST- 2020/10/07 00:00 [received]
PHST- 2020/10/30 00:00 [revised]
PHST- 2020/11/02 00:00 [accepted]
PHST- 2020/11/11 01:03 [entrez]
PHST- 2020/11/12 06:00 [pubmed]
PHST- 2021/07/27 06:00 [medline]
PHST- 2020/11/01 00:00 [pmc-release]
AID - genes11111317 [pii]
AID - genes-11-01317 [pii]
AID - 10.3390/genes11111317 [doi]
PST - epublish
SO  - Genes (Basel). 2020 Nov 6;11(11):1317. doi: 10.3390/genes11111317.