PMID- 33172253
OWN - NLM
STAT- MEDLINE
DCOM- 20210527
LR  - 20210527
IS  - 1554-8937 (Electronic)
IS  - 1554-8929 (Linking)
VI  - 15
IP  - 11
DP  - 2020 Nov 20
TI  - Quorum Sensing Inhibition Attenuates the Virulence of the Plant Pathogen 
      Ralstonia solanacearum Species Complex.
PG  - 3050-3059
LID - 10.1021/acschembio.0c00752 [doi]
AB  - Strains of Ralstonia solanacearum species complex (RSSC) cause "bacterial wilt" 
      on a wide range of plant species and thus lead to marked economic losses in 
      agriculture. Quorum sensing (QS), a bacterial cell-cell communication mechanism, 
      controls the virulence of RSSC strains by regulating the production of 
      extracellular polysaccharide (EPS) and secondary metabolites, biofilm formation, 
      and cellular motility. R. solanacearum strain OE1-1 employs (R)-methyl 
      3-hydroxymyristate (3-OH MAME) as a QS signal, which is synthesized by the PhcB 
      methyltransferase and sensed by the PhcS/PhcRQ two-component system. We describe 
      the design, synthesis, and biological evaluation of inhibitors of the phc QS 
      system. Initial screening of a small set of QS signal analogues revealed that 
      methyl 3-hydroxy-8-phenyloctanoate, named, PQI-1 (phc quorum sensing 
      inhibitor-1), inhibited biofilm formation by strain OE1-1. To improve its 
      inhibitory activity, the derivatives of PQI-1 were synthesized, and their QS 
      inhibition activities were evaluated. PQIs-2-5 evolved from PQI-1 more strongly 
      inhibited not only biofilm formation but also the production of ralfuranone and 
      EPS. Furthermore, RNA-Seq analysis revealed that the PQIs effectively inhibited 
      QS-dependent gene expression and repression in strain OE1-1. On the other hand, 
      the PQIs did not affect the canonical QS systems of the representative reporter 
      bacteria. These antagonists, especially PQI-5, reduced wilting symptoms of the 
      tomato plants infected with strain OE1-1. Taken together, we suggest that 
      targeting the phc QS system has potential for the development of chemicals that 
      protect agricultural crops from bacterial wilt disease.
FAU - Yoshihara, Ayaka
AU  - Yoshihara A
AD  - Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 
      1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
FAU - Shimatani, Mika
AU  - Shimatani M
AD  - Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 
      1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
FAU - Sakata, Megumi
AU  - Sakata M
AD  - Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 
      1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
FAU - Takemura, Chika
AU  - Takemura C
AD  - Laboratory of Plant Pathology and Biotechnology, Kochi University, 200 Otsu, 
      Monobe, Nanko-ku, Kochi 783-8502, Japan.
FAU - Senuma, Wakana
AU  - Senuma W
AD  - Laboratory of Plant Pathology and Biotechnology, Kochi University, 200 Otsu, 
      Monobe, Nanko-ku, Kochi 783-8502, Japan.
FAU - Hikichi, Yasufumi
AU  - Hikichi Y
AD  - Laboratory of Plant Pathology and Biotechnology, Kochi University, 200 Otsu, 
      Monobe, Nanko-ku, Kochi 783-8502, Japan.
FAU - Kai, Kenji
AU  - Kai K
AUID- ORCID: 0000-0002-4036-9959
AD  - Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 
      1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201110
PL  - United States
TA  - ACS Chem Biol
JT  - ACS chemical biology
JID - 101282906
RN  - 0 (Caprylates)
RN  - 0 (Myristates)
RN  - 0 (methyl 3-hydroxymyristate)
SB  - IM
MH  - Biofilms/drug effects
MH  - Caprylates/chemistry/*pharmacology
MH  - Myristates/metabolism
MH  - Plant Diseases/*microbiology/prevention & control
MH  - Quorum Sensing/*drug effects
MH  - Ralstonia solanacearum/*drug effects/pathogenicity
MH  - Virulence/drug effects
EDAT- 2020/11/12 06:00
MHDA- 2021/05/28 06:00
CRDT- 2020/11/11 05:34
PHST- 2020/11/12 06:00 [pubmed]
PHST- 2021/05/28 06:00 [medline]
PHST- 2020/11/11 05:34 [entrez]
AID - 10.1021/acschembio.0c00752 [doi]
PST - ppublish
SO  - ACS Chem Biol. 2020 Nov 20;15(11):3050-3059. doi: 10.1021/acschembio.0c00752. 
      Epub 2020 Nov 10.