PMID- 33174655
OWN - NLM
STAT- MEDLINE
DCOM- 20201210
LR  - 20201214
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Print)
IS  - 0960-7722 (Linking)
VI  - 53
IP  - 12
DP  - 2020 Dec
TI  - Generation of universal and hypoimmunogenic human pluripotent stem cells.
PG  - e12946
LID - 10.1111/cpr.12946 [doi]
LID - e12946
AB  - There is a need to store very large numbers of conventional human pluripotent 
      stem cell (hPSC) lines for their off-the-shelf usage in stem cell therapy. 
      Therefore, it is valuable to generate "universal" or "hypoimmunogenic" hPSCs with 
      gene-editing technology by knocking out or in immune-related genes. A few 
      universal or hypoimmunogenic hPSC lines should be enough to store for their 
      off-the-shelf usage. Here, we overview and discuss how to prepare universal or 
      hypoimmunogenic hPSCs and their disadvantages. beta2-Microglobulin-knockout hPSCs 
      did not harbour human leukocyte antigen (HLA)-expressing class I cells but rather 
      activated natural killer (NK) cells. To avoid NK cell and macrophage activities, 
      homozygous hPSCs expressing a single allele of an HLA class I molecule, such as 
      HLA-C, were developed. Major HLA class I molecules were knocked out, and PD-L1, 
      HLA-G and CD47 were knocked in hPSCs using CRISPR/Cas9 gene editing. These cells 
      escaped activation of not only T cells but also NK cells and macrophages, 
      generating universal hPSCs.
CI  - (c) 2020 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd.
FAU - Ye, Qingsong
AU  - Ye Q
AUID- ORCID: 0000-0002-2868-9247
AD  - School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
AD  - Center of Regenerative Medicine, Renmin Hospital of Wuhan University, Wuhan, 
      China.
AD  - Skeletal Biology Research Center, Department of Oral Maxillofacial Surgery, 
      Massachusetts General Hospital & Harvard School of Dental Medicine, Boston, MA, 
      USA.
FAU - Sung, Tzu-Cheng
AU  - Sung TC
AUID- ORCID: 0000-0003-4052-8048
AD  - School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, 
      Wenzhou, China.
AD  - Department of Chemical and Materials Engineering, National Central University, 
      Taoyuan, Taiwan.
FAU - Yang, Jen-Ming
AU  - Yang JM
AD  - Department of Chemical and Materials Engineering, Chang Gung University, Taoyuan, 
      Taiwan.
FAU - Ling, Qing-Dong
AU  - Ling QD
AUID- ORCID: 0000-0002-6432-6179
AD  - Cathay Medical Research Institute, Cathay General Hospital, Taipei, Taiwan.
FAU - He, Yan
AU  - He Y
AUID- ORCID: 0000-0002-0949-8525
AD  - School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
FAU - Higuchi, Akon
AU  - Higuchi A
AUID- ORCID: 0000-0003-2970-8531
AD  - School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, 
      Wenzhou, China.
AD  - Department of Chemical and Materials Engineering, National Central University, 
      Taoyuan, Taiwan.
AD  - Wenzhou Institute, University of Chinese Academy of Science, Wenzhou, China.
AD  - Department of Chemical Engineering and R&D Center for Membrane Technology, Chung 
      Yuan Christian University, Taoyuan, Taiwan.
AD  - Center for Emergent Matter Science, Riken, Saitama, Japan.
LA  - eng
GR  - 108-2221-E-008-037/Ministry of Science and Technology, Taiwan/
GR  - 108-2221-E-008-057/Ministry of Science and Technology, Taiwan/
GR  - 81701032/National Natural Science Foundation of China/
GR  - 81871503/National Natural Science Foundation of China/
GR  - 18K05251/Japan Society for the Promotion of Science/
PT  - Journal Article
PT  - Review
DEP - 20201111
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
SB  - IM
MH  - CRISPR-Cas Systems/*genetics
MH  - Family Characteristics
MH  - *Gene Editing
MH  - Humans
MH  - Killer Cells, Natural/*cytology
MH  - Pluripotent Stem Cells/*cytology
MH  - Stem Cell Transplantation/methods
PMC - PMC7705897
OTO - NOTNLM
OT  - regenerative medicine
OT  - stem cells
COIS- The authors declare no conflicts of interest.
EDAT- 2020/11/12 06:00
MHDA- 2020/12/15 06:00
PMCR- 2020/11/11
CRDT- 2020/11/11 08:39
PHST- 2020/10/04 00:00 [received]
PHST- 2020/10/13 00:00 [accepted]
PHST- 2020/11/12 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/11/11 08:39 [entrez]
PHST- 2020/11/11 00:00 [pmc-release]
AID - CPR12946 [pii]
AID - 10.1111/cpr.12946 [doi]
PST - ppublish
SO  - Cell Prolif. 2020 Dec;53(12):e12946. doi: 10.1111/cpr.12946. Epub 2020 Nov 11.