PMID- 33175512
OWN - NLM
STAT- MEDLINE
DCOM- 20210401
LR  - 20210401
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 68
IP  - 49
DP  - 2020 Dec 9
TI  - Purification, Characterization, and Three-Dimensional Structure Prediction of 
      Paramyosin, a Novel Allergen of Rapana venosa.
PG  - 14632-14642
LID - 10.1021/acs.jafc.0c04418 [doi]
AB  - Paramyosin (PM) is an important structural protein in molluscan muscles. However, 
      as an important allergen, there is a little information on PM in the molluscs. In 
      this study, a 99 kDa molecular weight allergen protein was purified from Rapana 
      venosa and confirmed as PM by mass spectrometry. The results of immunoglobulin E 
      (IgE)-binding activity and physicochemical characterization showed that R. venosa 
      PM could react with a specific IgE of the sera from sea snail-allergic patients, 
      and the IgE-binding activity could be reduced by thermal treatment. The 
      full-length cDNA of R. venosa PM was cloned, which encodes 859 amino acid 
      residues, and it has a higher homology among molluscan species. According to the 
      circular dichroism results, Fourier transform infrared, and 2D and 3D structure 
      analysis, both PM and tropomyosin are conserved proteins, which are mainly 
      composed of the alpha-helix structure. These results are significant for better 
      understanding the anaphylactic reactions in sea snail-allergic patients and 
      allergy diagnosis.
FAU - Yu, Chuang
AU  - Yu C
AD  - College of Food Science and Engineering, Ocean University of China, No. 5, Yushan 
      Road, Qingdao, Shandong Province 266003, P. R. China.
FAU - Gao, Xiang
AU  - Gao X
AD  - Department of Allergy, The Affiliated Hospital of Qingdao University, Qingdao, 
      Shandong Province 266071, P. R. China.
FAU - Lin, Hong
AU  - Lin H
AD  - College of Food Science and Engineering, Ocean University of China, No. 5, Yushan 
      Road, Qingdao, Shandong Province 266003, P. R. China.
FAU - Xu, Lili
AU  - Xu L
AUID- ORCID: 0000-0001-8759-1568
AD  - College of Food Science and Engineering, Ocean University of China, No. 5, Yushan 
      Road, Qingdao, Shandong Province 266003, P. R. China.
FAU - Ahmed, Ishfaq
AU  - Ahmed I
AD  - College of Food Science and Engineering, Ocean University of China, No. 5, Yushan 
      Road, Qingdao, Shandong Province 266003, P. R. China.
FAU - Khan, Mati Ullah
AU  - Khan MU
AD  - College of Food Science and Engineering, Ocean University of China, No. 5, Yushan 
      Road, Qingdao, Shandong Province 266003, P. R. China.
FAU - Xu, Mengyao
AU  - Xu M
AD  - College of Food Science and Engineering, Ocean University of China, No. 5, Yushan 
      Road, Qingdao, Shandong Province 266003, P. R. China.
FAU - Chen, Yan
AU  - Chen Y
AD  - NHC Key Laboratory of Food Safety Risk Assessment, Chinese Academy of Medical 
      Science Research Unit, China National Center for Food Safety Risk Assessment, 
      Beijing 100021, P. R. China.
FAU - Li, Zhenxing
AU  - Li Z
AUID- ORCID: 0000-0002-7866-681X
AD  - College of Food Science and Engineering, Ocean University of China, No. 5, Yushan 
      Road, Qingdao, Shandong Province 266003, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20201111
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Allergens)
RN  - 0 (Tropomyosin)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*chemistry/genetics/*immunology/isolation & purification
MH  - Amino Acid Sequence
MH  - Animals
MH  - Gastropoda/chemistry/genetics/*immunology
MH  - Immunoglobulin E/immunology
MH  - Protein Conformation
MH  - Sequence Alignment
MH  - Tropomyosin/*chemistry/genetics/*immunology/isolation & purification
OTO - NOTNLM
OT  - Rapana venosa
OT  - allergen
OT  - paramyosin
OT  - purification
EDAT- 2020/11/12 06:00
MHDA- 2021/04/02 06:00
CRDT- 2020/11/11 17:11
PHST- 2020/11/12 06:00 [pubmed]
PHST- 2021/04/02 06:00 [medline]
PHST- 2020/11/11 17:11 [entrez]
AID - 10.1021/acs.jafc.0c04418 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2020 Dec 9;68(49):14632-14642. doi: 10.1021/acs.jafc.0c04418. 
      Epub 2020 Nov 11.