PMID- 33176947
OWN - NLM
STAT- MEDLINE
DCOM- 20201218
LR  - 20201218
IS  - 1873-6424 (Electronic)
IS  - 0269-7491 (Linking)
VI  - 268
IP  - Pt A
DP  - 2021 Jan 1
TI  - Effects of nanoplastics on energy metabolism in the oriental river prawn 
      (Macrobrachium nipponense).
PG  - 115890
LID - S0269-7491(20)36579-9 [pii]
LID - 10.1016/j.envpol.2020.115890 [doi]
AB  - Nanoplastics are common pollutants in aquatic environments and have attracted 
      widespread research attention. However, few studies focus on the effects of 
      nanoplastic exposure on energy metabolism in crustaceans. Accordingly, we exposed 
      juvenile oriental river prawns (Macrobrachium nipponense) to different 
      concentrations of 75-nm polystyrene nanoplastics (0, 5, 10, 20, and 40 mg/L) for 
      7, 14, 21, or 28 days. Thereafter, the effects of nanoplastic exposure on 
      metabolite content, energy metabolism-related enzyme activity, and gene 
      expression were evaluated. Our results showed that (1) with increasing 
      nanoplastic concentration and exposure time, the survival rate decreased, while 
      weight gain rate and molting number increased and then decreased; glycogen, 
      triglyceride, and total cholesterol content all declined while lactic acid 
      content increased with higher exposure to nanoplastic concentrations; (2) the 
      activities of acetyl-CoA carboxylase (ACC), hexokinase (HK), carnitine palmitoyl 
      transferase-1, pyruvate kinase (PK), lipase, and fatty acid synthase tended to 
      decrease, while the activity of lactate dehydrogenase (LDH) increased. In 
      particular, the activity of 6-phosphofructokinase exposed to 5 mg/L nanoplastics 
      increased significantly (P < 0.05). (3) Expression of the metabolism-related 
      genes 6-phosphate glucokinase (G-6-Pase), HK, PK, ACC, Acetyl-CoA-binding protein 
      (ACBP), CPT-1, and fatty-acid-binding protein 10 (FABP 10) increased and then 
      decreased, while expression of the LDH gene showed an upward trend. These results 
      indicate that nanoplastics affect growth, enzyme activity, and the gene 
      expression of energy metabolism in M. nipponense, and that high concentrations of 
      nanoplastics have a negative impact on energy metabolism.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Li, Yiming
AU  - Li Y
AD  - School of Life Science, East China Normal University, Shanghai, 200241, China.
FAU - Liu, Zhiquan
AU  - Liu Z
AD  - School of Life Science, East China Normal University, Shanghai, 200241, China.
FAU - Yang, Yuan
AU  - Yang Y
AD  - School of Life Science, East China Normal University, Shanghai, 200241, China.
FAU - Jiang, Qichen
AU  - Jiang Q
AD  - Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing, 210017, 
      China.
FAU - Wu, Donglei
AU  - Wu D
AD  - School of Life Science, East China Normal University, Shanghai, 200241, China.
FAU - Huang, Youhui
AU  - Huang Y
AD  - School of Life Science, East China Normal University, Shanghai, 200241, China.
FAU - Jiao, Yang
AU  - Jiao Y
AD  - School of Life Science, East China Normal University, Shanghai, 200241, China.
FAU - Chen, Qiang
AU  - Chen Q
AD  - School of Life Science, East China Normal University, Shanghai, 200241, China.
FAU - Huang, Yinying
AU  - Huang Y
AD  - School of Life Science, East China Normal University, Shanghai, 200241, China.
FAU - Zhao, Yunlong
AU  - Zhao Y
AD  - School of Life Science, East China Normal University, Shanghai, 200241, China; 
      State Key Laboratory of Estuarine and Coastal Research, East China Normal 
      University, Shanghai, 200241, China. Electronic address: ylzhao426@163.com.
LA  - eng
PT  - Journal Article
DEP - 20201030
PL  - England
TA  - Environ Pollut
JT  - Environmental pollution (Barking, Essex : 1987)
JID - 8804476
RN  - 0 (2-cyclohexylidenhydrazo-4-phenyl-thiazole)
RN  - 0 (Microplastics)
RN  - 0 (Thiazoles)
SB  - IM
MH  - Animals
MH  - Energy Metabolism
MH  - Microplastics
MH  - Molting
MH  - *Palaemonidae
MH  - Thiazoles
OTO - NOTNLM
OT  - Energy metabolism
OT  - Enzyme activity
OT  - Gene expression
OT  - Macrobrachium nipponense
OT  - Nanoplastics
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/11/13 06:00
MHDA- 2020/12/19 06:00
CRDT- 2020/11/12 05:32
PHST- 2020/04/22 00:00 [received]
PHST- 2020/10/10 00:00 [revised]
PHST- 2020/10/15 00:00 [accepted]
PHST- 2020/11/13 06:00 [pubmed]
PHST- 2020/12/19 06:00 [medline]
PHST- 2020/11/12 05:32 [entrez]
AID - S0269-7491(20)36579-9 [pii]
AID - 10.1016/j.envpol.2020.115890 [doi]
PST - ppublish
SO  - Environ Pollut. 2021 Jan 1;268(Pt A):115890. doi: 10.1016/j.envpol.2020.115890. 
      Epub 2020 Oct 30.