PMID- 33181164
OWN - NLM
STAT- MEDLINE
DCOM- 20201223
LR  - 20201223
IS  - 1879-3150 (Electronic)
IS  - 0041-0101 (Linking)
VI  - 189
DP  - 2021 Jan 15
TI  - Pterostilbene inhibits deoxynivalenol-induced oxidative stress and inflammatory 
      response in bovine mammary epithelial cells.
PG  - 10-18
LID - S0041-0101(20)30446-3 [pii]
LID - 10.1016/j.toxicon.2020.11.002 [doi]
AB  - More and more studies have showed that tricothecene mycotoxin, deoxynivalenol 
      (DON) caused cytotoxicity in mammary alveolar cells-large T antigen cells 
      (MAC-T). Therefore, research on reducing the cytotoxicity of DON has gradually 
      attracted attention. In this study, we aim to explore the potential of 
      pterostilbene (PTE) to protect MAC-T cells from DON-induced oxidative stress and 
      inflammatory response. MAC-T cells were treated with 0.25 mug/mL DON or 
      2.0504 mug/mL PTE or 0.25 mug/mL DON and 2.0504 mug/mL PTE together, incubated for 
      9 h. PTE effectively improved cell viability, cell proliferation and total 
      antioxidant capacity (T-AOC), reduced reactive oxygen species (ROS) production 
      and malondialdehyde (MDA), and improved glutathione (GSH) depletion. Moreover, 
      PTE effectively regulated the mRNA levels of nuclear factor erythroid-2-related 
      factor 2 (Nrf2), kelch-like ech-associated protein 1 (Keap1), superoxide 
      dismutase 1 (SOD1) and superoxide dismutase 2 (SOD2). PTE significantly inhibited 
      nuclear factor kappa-B P65 (NF-kappaB P65), nuclear factor kappa-B P50 (NF-kappaB P50), 
      cyclooxygenase-2 (COX-2), interleukin-1beta (IL-1beta), interleukin-6 (IL-6) and 
      monocyte chemotactic protein 1 (MCP-1) mRNA levels in DON-induced MAC-T cells. 
      PTE also significantly reduced inducible nitric oxide synthase (iNOS) and nitric 
      oxide (NO) levels in DON-induced MAC-T cells. Additionally, ELISA revealed that 
      PTE inhibited the expression of tumor necrosis factor-alpha (TNF-alpha) and IL-6 proteins 
      produced in DON-induced MAC-T cells. These findings together provided strong 
      evidence to support that PTE can effectively alleviate the damage to cells caused 
      by DON, and it may be used as an effective anti-inflammatory and antioxidant to 
      prevent the damage of mycotoxins to the animal body.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Zhang, Jing
AU  - Zhang J
AD  - College of Animal Sciences, Jilin University, Changchun, 130062, China; Key 
      Laboratory of Zoonosis Research, Ministry of Education, Jilin University, 
      Changchun, 130062, China.
FAU - Wang, JunMei
AU  - Wang J
AD  - Low Carbon Breeding Cattle and Safety Production University Key Laboratory of 
      Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, 
      Chengdu, 611130, China.
FAU - Fang, HengTong
AU  - Fang H
AD  - College of Animal Sciences, Jilin University, Changchun, 130062, China.
FAU - Yu, Hao
AU  - Yu H
AD  - College of Animal Sciences, Jilin University, Changchun, 130062, China.
FAU - Zhao, Yun
AU  - Zhao Y
AD  - College of Animal Sciences, Jilin University, Changchun, 130062, China.
FAU - Shen, JingLin
AU  - Shen J
AD  - College of Animal Sciences, Jilin University, Changchun, 130062, China.
FAU - Zhou, ChangHai
AU  - Zhou C
AD  - College of Animal Sciences, Jilin University, Changchun, 130062, China.
FAU - Jin, YongCheng
AU  - Jin Y
AD  - College of Animal Sciences, Jilin University, Changchun, 130062, China. 
      Electronic address: ycjin78@163.com.
LA  - eng
PT  - Journal Article
DEP - 20201109
PL  - England
TA  - Toxicon
JT  - Toxicon : official journal of the International Society on Toxinology
JID - 1307333
RN  - 0 (Antioxidants)
RN  - 0 (Kelch-Like ECH-Associated Protein 1)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Stilbenes)
RN  - 0 (Trichothecenes)
RN  - 26R60S6A5I (pterostilbene)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.15.1.1 (superoxide dismutase 2)
RN  - JT37HYP23V (deoxynivalenol)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Cattle
MH  - Cell Survival/drug effects
MH  - Epithelial Cells
MH  - Kelch-Like ECH-Associated Protein 1
MH  - NF-E2-Related Factor 2
MH  - Oxidative Stress/*drug effects
MH  - Reactive Oxygen Species
MH  - Stilbenes/*pharmacology
MH  - Superoxide Dismutase
MH  - Trichothecenes/*toxicity
OTO - NOTNLM
OT  - Bovine mammary epithelial cells
OT  - Deoxynivalenol
OT  - Inflammatory response
OT  - Oxidative stress
OT  - Pterostilbene
EDAT- 2020/11/13 06:00
MHDA- 2020/12/29 06:00
CRDT- 2020/11/12 20:10
PHST- 2020/08/17 00:00 [received]
PHST- 2020/10/19 00:00 [revised]
PHST- 2020/11/08 00:00 [accepted]
PHST- 2020/11/13 06:00 [pubmed]
PHST- 2020/12/29 06:00 [medline]
PHST- 2020/11/12 20:10 [entrez]
AID - S0041-0101(20)30446-3 [pii]
AID - 10.1016/j.toxicon.2020.11.002 [doi]
PST - ppublish
SO  - Toxicon. 2021 Jan 15;189:10-18. doi: 10.1016/j.toxicon.2020.11.002. Epub 2020 Nov 
      9.