PMID- 33181211 OWN - NLM STAT- MEDLINE DCOM- 20210421 LR - 20210421 IS - 1879-0003 (Electronic) IS - 0141-8130 (Linking) VI - 167 DP - 2021 Jan 15 TI - Study on the anti-infection ability of vancomycin cationic liposome combined with polylactide fracture internal fixator. PG - 834-844 LID - S0141-8130(20)34964-3 [pii] LID - 10.1016/j.ijbiomac.2020.11.039 [doi] AB - A polylactide composite fracture fixator loaded with vancomycin cationic liposome (PLA@VL) was prepared by reverse evaporation method. The method of cationic liposome encapsulating vancomycin could effectively improve antibacterial property and achieve drug sustained release effect, so as to reduce toxicity of antibiotics in vivo. Scanning electron microscope (SEM) was used to observe morphology and Fourier transform infrared spectroscopy (FTIR) was used to detect the composition of the internal fixator. In vitro drug release model, in vitro degradation model and body fluid osteogenesis model were designed in this study. On the other hand, the experiments of inhibition zone and MC3T3-E1 osteoblasts in mice were conducted to explore antibacterial property, cell activity and adhesion of the PLA@VL composite internal fixator. Alkaline phosphatase (ALP) staining method and alizarin red assay were used to detect the osteogenic induction ability of the composite internal fixator. Finally, mice fracture models were established to verify osteogenic and anti-infection abilities of the composite internal fixator in vivo. The results showed that MC3T3-E1 cells had better adhesion and proliferation abilities on the PLA@VL composite internal fixator than on the PLA fixator, which indicated that the PLA@VL composite internal fixator possessed excellent osteogenic and anti-infection abilities both in vivo and in vitro. Therefore, the above experiments showed that the fracture internal fixator combined with vancomycin cationic liposome had better biocompatibility, antibacterial ability and osteogenic ability, which provides a promising anti-infection material for the clinical field of fracture. CI - Copyright (c) 2020. Published by Elsevier B.V. FAU - Cai, Weibin AU - Cai W AD - Department of Cardiothoracic Surgery, Xiamen University Affiliated Southeast Hospital, Zhangzhou 363000, China. FAU - Liu, Jiandong AU - Liu J AD - Department of Anesthesiology, Xiamen University Affiliated Southeast Hospital, Zhangzhou 363000, China. FAU - Zheng, Liling AU - Zheng L AD - Department of Cardiothoracic Surgery, Quanzhou First Hospital, Fujian Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou, Fujian 362000, China. FAU - Xu, Zhiyang AU - Xu Z AD - Department of Cardiothoracic Surgery, The First Hospital of Putian City, Putian, Fujian 351100, China. FAU - Chen, Jianming AU - Chen J AD - Department of Cardiothoracic Surgery, Xiamen University Affiliated Southeast Hospital, Zhangzhou 363000, China. FAU - Zhong, Jing AU - Zhong J AD - Department of Cardiothoracic Surgery, Xiamen University Affiliated Southeast Hospital, Zhangzhou 363000, China. FAU - Song, Zhiming AU - Song Z AD - Department of Cardiothoracic Surgery, Xiamen University Affiliated Southeast Hospital, Zhangzhou 363000, China. FAU - Xu, Xiaoping AU - Xu X AD - Department of Cardiothoracic Surgery, Xiamen University Affiliated Southeast Hospital, Zhangzhou 363000, China. FAU - Chen, Songlin AU - Chen S AD - Department of Cardiothoracic Surgery, Xiamen University Affiliated Southeast Hospital, Zhangzhou 363000, China. FAU - Jiao, Changjie AU - Jiao C AD - Department of Cardiothoracic Surgery, Xiamen University Affiliated Southeast Hospital, Zhangzhou 363000, China. FAU - Guo, Junhua AU - Guo J AD - Department of Cardiothoracic Surgery, Xiamen University Affiliated Southeast Hospital, Zhangzhou 363000, China. FAU - Yi, Yunfeng AU - Yi Y AD - Department of Cardiothoracic Surgery, Xiamen University Affiliated Southeast Hospital, Zhangzhou 363000, China. Electronic address: yiyunfeng199801@163.com. FAU - Zhang, Yanmei AU - Zhang Y AD - Department of Pharmacology, Shantou University Medical College, 22 Xin Ling Road, Shantou, Guangdong 515041, China. Electronic address: zhangyanmei1678@163.com. LA - eng PT - Journal Article DEP - 20201109 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 RN - 0 (Anti-Bacterial Agents) RN - 0 (Biocompatible Materials) RN - 0 (Biomarkers) RN - 0 (Liposomes) RN - 0 (Polyesters) RN - 459TN2L5F5 (poly(lactide)) RN - 6Q205EH1VU (Vancomycin) RN - EC 3.1.3.1 (Alkaline Phosphatase) SB - IM MH - Alkaline Phosphatase/metabolism MH - Animals MH - Anti-Bacterial Agents/*administration & dosage/chemistry MH - Biocompatible Materials/chemistry MH - Biomarkers MH - Cell Adhesion/drug effects MH - Cell Survival/drug effects MH - Cells, Cultured MH - Drug Liberation MH - *Internal Fixators MH - Liposomes/*chemistry/ultrastructure MH - Mice MH - Microbial Sensitivity Tests MH - NIH 3T3 Cells MH - Osteogenesis/drug effects MH - Polyesters/*analysis MH - Solubility MH - Spectrum Analysis MH - Tissue Engineering MH - Tissue Scaffolds/chemistry MH - Vancomycin/*administration & dosage/chemistry OTO - NOTNLM OT - Cationic liposome OT - Polylactide (PLA) OT - Vancomycin COIS- Declaration of competing interest The authors declare that they have no potential conflicts of interest. EDAT- 2020/11/13 06:00 MHDA- 2021/04/22 06:00 CRDT- 2020/11/12 20:10 PHST- 2020/06/18 00:00 [received] PHST- 2020/10/25 00:00 [revised] PHST- 2020/11/06 00:00 [accepted] PHST- 2020/11/13 06:00 [pubmed] PHST- 2021/04/22 06:00 [medline] PHST- 2020/11/12 20:10 [entrez] AID - S0141-8130(20)34964-3 [pii] AID - 10.1016/j.ijbiomac.2020.11.039 [doi] PST - ppublish SO - Int J Biol Macromol. 2021 Jan 15;167:834-844. doi: 10.1016/j.ijbiomac.2020.11.039. Epub 2020 Nov 9.