PMID- 33182066 OWN - NLM STAT- MEDLINE DCOM- 20210602 LR - 20210602 IS - 1878-1705 (Electronic) IS - 1567-5769 (Linking) VI - 88 DP - 2020 Nov TI - Puerarin inhibits Mycoplasma gallisepticum (MG-HS)-induced inflammation and apoptosis via suppressing the TLR6/MyD88/NF-kappaB signal pathway in chicken. PG - 106993 LID - S1567-5769(20)30829-8 [pii] LID - 10.1016/j.intimp.2020.106993 [doi] AB - Mycoplasma gallisepticum (MG) is the primary etiological agent of chicken chronic respiratory disease (CRD), which mainly causes inflammatory damage of the host respiratory system. Previous studies suggest that puerarin (PUE) plays a pivotal regulatory role in inflammatory diseases, whereas the impacts of PUE on MG-induced inflammation remain unclear. This study investigated the effects of PUE on MG-HS infection in vitro and in vivo and indicated its potential therapeutic and preventive value. Experimental results showed that PUE significantly suppressed pMGA1.2 expression, promoted MG-infected cell proliferation and cell cycle process by reducing apoptosis. Histopathological examination of lung tissue showed severe histopathological lesions including thickened alveolar walls, narrowed alveolar cavity, and inflammatory cell infiltration in the MG-infected chicken group. However, PUE treatment significantly ameliorated MG-induced pathological damage in lung. Compared to the MG-infected group, PUE effectively inhibited the expression of MG-induced inflammatory genes, including tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), cytokines interleukin-6 (IL-6), toll-like receptor 6 (TLR6), myeloid differentiation primary response gene 88 (MyD88) and nuclear factor kappaB (NF-kappaB). Moreover, PUE dose-dependently inhibited MG-induced NF-kappaB p65 to enter the cell nucleus. In conclusion, our findings indicate that PUE treatment can efficiently inhibit MG-induced inflammatory response and apoptosis, and protect the lung from MG infection-induced damage by inhibiting the TLR6/MyD88/NF-kappaB signaling pathway activation. The study suggests that PUE may be a potential anti-inflammatory agent defense againstMGinfection in chicken. CI - Copyright (c) 2020 Elsevier B.V. All rights reserved. FAU - Niu, Lumeng AU - Niu L AD - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. FAU - Luo, Ronglong AU - Luo R AD - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. FAU - Zou, Mengyun AU - Zou M AD - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. FAU - Sun, Yingfei AU - Sun Y AD - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. FAU - Fu, Yali AU - Fu Y AD - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. FAU - Wang, Yingjie AU - Wang Y AD - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. FAU - Peng, Xiuli AU - Peng X AD - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: xlpengsishun@mail.hzau.edu.cn. LA - eng PT - Journal Article DEP - 20200920 PL - Netherlands TA - Int Immunopharmacol JT - International immunopharmacology JID - 100965259 RN - 0 (Bacterial Proteins) RN - 0 (Isoflavones) RN - 0 (Myeloid Differentiation Factor 88) RN - 0 (NF-kappa B) RN - 0 (Toll-Like Receptor 6) RN - 0 (Vasodilator Agents) RN - Z9W8997416 (puerarin) SB - IM MH - Animals MH - Apoptosis/drug effects MH - Bacterial Proteins/genetics/metabolism MH - Cell Cycle/drug effects MH - Cell Line MH - Cell Proliferation/drug effects MH - Cell Survival/drug effects MH - Chick Embryo MH - Gene Expression Regulation, Bacterial/drug effects MH - Inflammation/drug therapy MH - Isoflavones/chemistry/*pharmacology MH - Molecular Structure MH - *Mycoplasma gallisepticum MH - Myeloid Differentiation Factor 88/genetics/*metabolism MH - NF-kappa B/genetics/*metabolism MH - Signal Transduction MH - Toll-Like Receptor 6/genetics/*metabolism MH - Vasodilator Agents/pharmacology OTO - NOTNLM OT - Apoptosis OT - Inflammation OT - Mycoplasma gallisepticum (MG-HS strain) OT - Puerarin OT - TLR6/MyD88/NF-kappaB signaling pathway COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2020/11/14 06:00 MHDA- 2021/06/03 06:00 CRDT- 2020/11/13 01:01 PHST- 2020/03/19 00:00 [received] PHST- 2020/08/22 00:00 [revised] PHST- 2020/09/07 00:00 [accepted] PHST- 2020/11/14 06:00 [pubmed] PHST- 2021/06/03 06:00 [medline] PHST- 2020/11/13 01:01 [entrez] AID - S1567-5769(20)30829-8 [pii] AID - 10.1016/j.intimp.2020.106993 [doi] PST - ppublish SO - Int Immunopharmacol. 2020 Nov;88:106993. doi: 10.1016/j.intimp.2020.106993. Epub 2020 Sep 20.