PMID- 33182075
OWN - NLM
STAT- MEDLINE
DCOM- 20210527
LR  - 20210527
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 88
DP  - 2020 Nov
TI  - FUN14 domain-containing 1-mediated mitophagy suppresses interleukin-1beta production 
      in macrophages.
PG  - 106964
LID - S1567-5769(20)31388-6 [pii]
LID - 10.1016/j.intimp.2020.106964 [doi]
AB  - Mitochondria play a critical role in triggering immune response. Although recent 
      evidence indicates that autophagy/mitophagy can suppress inflammation via 
      regulation of mitochondrial homeostasis, limited information is available 
      regarding physiological regulation of mitochondria-controlled inflammation. In 
      this study, we investigated FUN14 domain containing 1 (FUNDC1)-mediated mitophagy 
      in the regulation of interleukin-1beta (IL-1beta) in vitro and in vivo, wild-type 
      FUNDC1 and its mitophagy defective Y18A/L21A mutant were analyzed in bone 
      marrow-derived macrophages (BMDMs)for their effects on IL-1beta expression and 
      mitochondrial damage. The current study identified that LPS plus nigericin 
      stimulation induced NLR family pyrin domain containing 3 (NLRP3) inflammasome 
      activation, which was detected by IL-1beta expression. Moreover, FUNDC1-mediated 
      mitophagy promoted the alleviation of intracellular reactive oxygen species 
      (ROS). IL-1beta production was suppressed by the overexpression of wild-type FUNDC1, 
      but not the Y18A/L21A mutant. Our results suggest that FUNDC1 suppresses LPS plus 
      nigericin-mediated IL-1beta production through its regulatory effect on mitophagy, 
      which will greatly promote the understanding of mitophagy-related protein in the 
      regulation of immune response.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Huang, Jia
AU  - Huang J
AD  - Department of Intensive Care Unit, Second Hospital Affiliated to Southern 
      University of Science and Technology, Shenzhen Third People's Hospital, Shenzhen 
      518112, China.
FAU - Zhu, Tengfei
AU  - Zhu T
AD  - Department of Intensive Care Unit, Second Hospital Affiliated to Southern 
      University of Science and Technology, Shenzhen Third People's Hospital, Shenzhen 
      518112, China.
FAU - Rong, Rong
AU  - Rong R
AD  - Eye Center of Xiangya Hospital, Central South University, Changsha 410008, Hunan 
      Province, China.
FAU - You, Mengling
AU  - You M
AD  - Eye Center of Xiangya Hospital, Central South University, Changsha 410008, Hunan 
      Province, China.
FAU - Ji, Dan
AU  - Ji D
AD  - Eye Center of Xiangya Hospital, Central South University, Changsha 410008, Hunan 
      Province, China; Hunan Key Laboratory of Ophthalmogy, Central South University, 
      Changsha 410008, Hunan Province, China. Electronic address: 475393400@qq.com.
FAU - Li, Haibo
AU  - Li H
AD  - Eye Center of Xiangya Hospital, Central South University, Changsha 410008, Hunan 
      Province, China; Hunan Key Laboratory of Ophthalmogy, Central South University, 
      Changsha 410008, Hunan Province, China. Electronic address: 
      lihaibo151@aliyun.com.
LA  - eng
PT  - Journal Article
DEP - 20200917
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (FUNDC1 protein, mouse)
RN  - 0 (IL1B protein, mouse)
RN  - 0 (Inflammasomes)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Reactive Oxygen Species)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - RRU6GY95IS (Nigericin)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Cells, Cultured
MH  - Inflammasomes/metabolism
MH  - Interleukin-1beta/*metabolism
MH  - Lipopolysaccharides
MH  - Macrophages/metabolism
MH  - Male
MH  - Membrane Proteins/*metabolism
MH  - Mice, Inbred C57BL
MH  - Mitochondrial Proteins/*metabolism
MH  - *Mitophagy
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - Nigericin/pharmacology
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - FUNDC1
OT  - IL-1beta
OT  - Macrophage
OT  - Mitophagy
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/11/14 06:00
MHDA- 2021/05/28 06:00
CRDT- 2020/11/13 01:01
PHST- 2020/05/02 00:00 [received]
PHST- 2020/08/14 00:00 [revised]
PHST- 2020/08/28 00:00 [accepted]
PHST- 2020/11/14 06:00 [pubmed]
PHST- 2021/05/28 06:00 [medline]
PHST- 2020/11/13 01:01 [entrez]
AID - S1567-5769(20)31388-6 [pii]
AID - 10.1016/j.intimp.2020.106964 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2020 Nov;88:106964. doi: 10.1016/j.intimp.2020.106964. Epub 
      2020 Sep 17.