PMID- 33187022
OWN - NLM
STAT- MEDLINE
DCOM- 20210707
LR  - 20210707
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 131
IP  - 1
DP  - 2021 Jul
TI  - Microbially induced carbonate precipitation via methanogenesis pathway by a 
      microbial consortium enriched from activated anaerobic sludge.
PG  - 236-256
LID - 10.1111/jam.14930 [doi]
AB  - AIMS: Various applications of microbially induced carbonate precipitation (MICP) 
      has been proposed. However, most studies use cultured pure strains to obtain 
      MICP, ignoring advantages of microbial consortia. The aims of this study were to: 
      (i) test the feasibility of a microbial consortium to produce MICP; (ii) identify 
      functional micro-organisms and their relationship; (iii) explain the MICP 
      mechanism; (iv) propose a way of applying the MICP technique to soil media. 
      METHODS AND RESULTS: Anaerobic sludge was used as the source of the microbial 
      consortium. A laboratory anaerobic sequencing batch reactor and beaker were used 
      to perform precipitation experiment. The microbial consortium produced MICP with 
      an efficiency of 96.6%. XRD and SEM analysis showed that the precipitation 
      composed of different-size calcite crystals. According to high-throughput 16S 
      rRNA gene sequencing, the functional micro-organisms included acetogenic 
      bacteria, acetate-oxidizing bacteria and archaea Methanosaeta and 
      Methanobacterium beijingense. The methanogenesis acetate degradation provides 
      dissolved inorganic carbon and increases pH for MICP. A series of reactions 
      catalysed by many enzymes and cofactors of methanogens and acetate-oxidizers are 
      involved in the acetate degradation. CONCLUSION: This work demonstrates the 
      feasibility of using the microbial consortium to achieve MICP from an 
      experimental and theoretical perspective. SIGNIFICANCE AND IMPACT OF THE STUDY: A 
      method of applying the microbial-consortium MICP to soil media is proposed. It 
      has the advantages of low cost, low environmental impact, treatment uniformity 
      and less limitations from natural soils. This method could be used to improve 
      mechanical properties, plug pores and fix harmful elements of soil media, etc.
CI  - (c) 2020 The Society for Applied Microbiology.
FAU - Su, F
AU  - Su F
AUID- ORCID: 0000-0001-5000-426X
AD  - School of Engineering and Technology, China University of Geosciences (Beijing), 
      Beijing, P. R. China.
FAU - Yang, Y Y
AU  - Yang YY
AD  - School of Engineering and Technology, China University of Geosciences (Beijing), 
      Beijing, P. R. China.
LA  - eng
GR  - 2017YFC0805008/National Key Research and Development Program of China/
GR  - 265201708/Fundamental Research Funds for the Central Universities/
GR  - 265201708/National Key Research and Development Program of China/
PT  - Journal Article
DEP - 20201230
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Acetates)
RN  - 0 (Carbon Compounds, Inorganic)
RN  - 0 (Carbonates)
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - H0G9379FGK (Calcium Carbonate)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Acetates/metabolism
MH  - Anaerobiosis
MH  - Archaea/*physiology
MH  - Bacteria, Anaerobic/*physiology
MH  - Calcium Carbonate/*metabolism
MH  - Carbon Compounds, Inorganic/metabolism
MH  - Carbonates/*metabolism
MH  - Chemical Precipitation
MH  - DNA, Bacterial/genetics
MH  - High-Throughput Nucleotide Sequencing
MH  - Hydrogen-Ion Concentration
MH  - Industrial Microbiology/methods
MH  - Metabolic Networks and Pathways
MH  - Methane/metabolism
MH  - *Microbial Consortia
MH  - Microbial Interactions
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/microbiology
MH  - Soil Microbiology
OTO - NOTNLM
OT  - MICP
OT  - acetate degradation
OT  - bio-cementation
OT  - calcium carbonate
OT  - methanogenesis
OT  - microbial consortium
EDAT- 2020/11/14 06:00
MHDA- 2021/07/08 06:00
CRDT- 2020/11/13 20:17
PHST- 2020/10/14 00:00 [revised]
PHST- 2020/05/10 00:00 [received]
PHST- 2020/11/04 00:00 [accepted]
PHST- 2020/11/14 06:00 [pubmed]
PHST- 2021/07/08 06:00 [medline]
PHST- 2020/11/13 20:17 [entrez]
AID - 10.1111/jam.14930 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2021 Jul;131(1):236-256. doi: 10.1111/jam.14930. Epub 2020 Dec 
      30.