PMID- 33187941
OWN - NLM
STAT- MEDLINE
DCOM- 20210520
LR  - 20220531
IS  - 2212-4411 (Electronic)
VI  - 131
IP  - 5
DP  - 2021 May
TI  - Cross-talk between synovial fibroblasts and chondrocytes in condylar hyperplasia: 
      an in vitro pilot study.
PG  - 558-564
LID - S2212-4403(20)31169-X [pii]
LID - 10.1016/j.oooo.2020.08.020 [doi]
AB  - OBJECTIVE: Increasing evidence indicates an interaction between the synovium and 
      the cartilage in the temporomandibular joint (TMJ) and other joints. We recently 
      demonstrated that the expression of proangiogenic factors was enhanced and that 
      of factors promoting matrix degradation was decreased in synovial fibroblasts in 
      condylar hyperplasia (CH). The aim of this study was to explore whether CH 
      chondrocytes can affect the expression of these factors of synovial fibroblasts 
      in a co-culture system. STUDY DESIGN: The expressions of vascular endothelial 
      growth factor (VEGF), cluster of differentiation 34 (CD34), fibroblast growth 
      factor 2 (FGF-2), and tissue inhibitor of metalloproteinase 1 (TIMP1) from CH 
      condylar tissues were observed by using immunohistochemical methods. Synovial 
      fibroblasts of control tissues were co-cultured with the chondrocytes of CH, and 
      protein expressions of VEGF, FGF-2, thrombospondin 1 (TSP1), matrix 
      metalloproteinase 3 (MMP3), and TIMP1 were examined by using Western blotting. 
      RESULTS: Positive staining for VEGF, CD34, FGF-2, and TIMP1 was found in the 
      hypertrophic cartilage layer of CH condylar tissues. Protein expressions of VEGF, 
      FGF-2, and TIMP1 were significantly increased in co-cultured synovial 
      fibroblasts, but TSP1 and MMP3 expressions were decreased. CONCLUSIONS: The 
      angiogenic factors and matrix degradation-related factors in synovial fibroblasts 
      co-cultured with CH chondrocytes showed the same trends as those in synovial 
      fibroblasts from CH tissue, suggesting potential cross-talk between synovial 
      fibroblasts and chondrocytes during CH progression.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Guo, Huilin
AU  - Guo H
AD  - State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & 
      Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School and 
      Hospital of Stomatology, Wuhan University, Wuhan, China; Department of Oral and 
      Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, 
      Wuhan, China.
FAU - Li, Huimin
AU  - Li H
AD  - State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & 
      Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School and 
      Hospital of Stomatology, Wuhan University, Wuhan, China.
FAU - Feng, Yaping
AU  - Feng Y
AD  - State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & 
      Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School and 
      Hospital of Stomatology, Wuhan University, Wuhan, China.
FAU - Ke, Jin
AU  - Ke J
AD  - State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & 
      Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School and 
      Hospital of Stomatology, Wuhan University, Wuhan, China; Department of Oral and 
      Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, 
      Wuhan, China.
FAU - Fang, Wei
AU  - Fang W
AD  - State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & 
      Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School and 
      Hospital of Stomatology, Wuhan University, Wuhan, China; Department of Oral and 
      Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, 
      Wuhan, China.
FAU - Li, Cheng
AU  - Li C
AD  - Department of Stomatology, Zhongnan Hospital of Wuhan University, Wuhan, China.
FAU - Long, Xing
AU  - Long X
AD  - Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, 
      Wuhan University, Wuhan, China. Electronic address: longxing@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200826
PL  - United States
TA  - Oral Surg Oral Med Oral Pathol Oral Radiol
JT  - Oral surgery, oral medicine, oral pathology and oral radiology
JID - 101576782
RN  - 0 (Antigens, CD34)
RN  - 0 (TIMP1 protein, human)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Antigens, CD34
MH  - Cells, Cultured
MH  - *Chondrocytes
MH  - Fibroblast Growth Factor 2
MH  - *Fibroblasts
MH  - Humans
MH  - Hyperplasia/pathology
MH  - Mandible/*pathology
MH  - Pilot Projects
MH  - Synovial Membrane/cytology
MH  - *Tissue Inhibitor of Metalloproteinase-1
MH  - Vascular Endothelial Growth Factor A
EDAT- 2020/11/15 06:00
MHDA- 2021/05/21 06:00
CRDT- 2020/11/14 05:23
PHST- 2020/02/29 00:00 [received]
PHST- 2020/08/14 00:00 [revised]
PHST- 2020/08/16 00:00 [accepted]
PHST- 2020/11/15 06:00 [pubmed]
PHST- 2021/05/21 06:00 [medline]
PHST- 2020/11/14 05:23 [entrez]
AID - S2212-4403(20)31169-X [pii]
AID - 10.1016/j.oooo.2020.08.020 [doi]
PST - ppublish
SO  - Oral Surg Oral Med Oral Pathol Oral Radiol. 2021 May;131(5):558-564. doi: 
      10.1016/j.oooo.2020.08.020. Epub 2020 Aug 26.