PMID- 33187979 OWN - NLM STAT- MEDLINE DCOM- 20201228 LR - 20210630 IS - 2375-2548 (Electronic) IS - 2375-2548 (Linking) VI - 6 IP - 50 DP - 2020 Dec TI - Distinct inflammatory profiles distinguish COVID-19 from influenza with limited contributions from cytokine storm. LID - 10.1126/sciadv.abe3024 [doi] LID - eabe3024 AB - We pursued a study of immune responses in coronavirus disease 2019 (COVID-19) and influenza patients. Compared to patients with influenza, patients with COVID-19 exhibited largely equivalent lymphocyte counts, fewer monocytes, and lower surface human leukocyte antigen (HLA)-class II expression on selected monocyte populations. Furthermore, decreased HLA-DR on intermediate monocytes predicted severe COVID-19 disease. In contrast to prevailing assumptions, very few (7 of 168) patients with COVID-19 exhibited cytokine profiles indicative of cytokine storm syndrome. After controlling for multiple factors including age and sample time point, patients with COVID-19 exhibited lower cytokine levels than patients with influenza. Up-regulation of IL-6, G-CSF, IL-1RA, and MCP1 predicted death in patients with COVID-19 but were not statistically higher than patients with influenza. Single-cell transcriptional profiling revealed profound suppression of interferon signaling among patients with COVID-19. When considered across the spectrum of peripheral immune profiles, patients with COVID-19 are less inflamed than patients with influenza. CI - Copyright (c) 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). FAU - Mudd, Philip A AU - Mudd PA AUID- ORCID: 0000-0002-3860-5473 AD - Department of Emergency Medicine, Washington University School of Medicine, Saint Louis, MO, USA. pmudd@wustl.edu paul.thomas@stjude.org ellebedy@wustl.edu. FAU - Crawford, Jeremy Chase AU - Crawford JC AUID- ORCID: 0000-0003-4096-6048 AD - Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN, USA. FAU - Turner, Jackson S AU - Turner JS AUID- ORCID: 0000-0002-4396-6265 AD - Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO, USA. FAU - Souquette, Aisha AU - Souquette A AUID- ORCID: 0000-0001-6197-5870 AD - Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN, USA. FAU - Reynolds, Daniel AU - Reynolds D AD - Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO, USA. FAU - Bender, Diane AU - Bender D AUID- ORCID: 0000-0002-8311-4390 AD - Bursky Center for Human Immunology and Immunotherapy Program, Washington University School of Medicine, Saint Louis, MO, USA. FAU - Bosanquet, James P AU - Bosanquet JP AD - Department of Critical Care, Missouri Baptist Medical Center, Saint Louis, MO, USA. FAU - Anand, Nitin J AU - Anand NJ AD - Department of Critical Care, Missouri Baptist Medical Center, Saint Louis, MO, USA. FAU - Striker, David A AU - Striker DA AD - Department of Critical Care, Missouri Baptist Medical Center, Saint Louis, MO, USA. FAU - Martin, R Scott AU - Martin RS AD - Department of Critical Care, Missouri Baptist Medical Center, Saint Louis, MO, USA. FAU - Boon, Adrianus C M AU - Boon ACM AUID- ORCID: 0000-0002-4700-8224 AD - Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO, USA. FAU - House, Stacey L AU - House SL AD - Department of Emergency Medicine, Washington University School of Medicine, Saint Louis, MO, USA. FAU - Remy, Kenneth E AU - Remy KE AD - Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO, USA. AD - Department of Pediatrics, Washington University School of Medicine, Saint Louis, MO, USA. AD - Department of Anesthesiology, Washington University School of Medicine, Saint Louis, MO, USA. FAU - Hotchkiss, Richard S AU - Hotchkiss RS AD - Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO, USA. AD - Department of Anesthesiology, Washington University School of Medicine, Saint Louis, MO, USA. AD - Department of Surgery, Washington University School of Medicine, Saint Louis, MO, USA. FAU - Presti, Rachel M AU - Presti RM AUID- ORCID: 0000-0002-5469-0727 AD - Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO, USA. FAU - O'Halloran, Jane A AU - O'Halloran JA AD - Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO, USA. FAU - Powderly, William G AU - Powderly WG AUID- ORCID: 0000-0001-7808-3086 AD - Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO, USA. FAU - Thomas, Paul G AU - Thomas PG AUID- ORCID: 0000-0001-7955-0256 AD - Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN, USA. pmudd@wustl.edu paul.thomas@stjude.org ellebedy@wustl.edu. FAU - Ellebedy, Ali H AU - Ellebedy AH AUID- ORCID: 0000-0002-6129-2532 AD - Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO, USA. pmudd@wustl.edu paul.thomas@stjude.org ellebedy@wustl.edu. AD - Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO, USA. AD - Bursky Center for Human Immunology and Immunotherapy Program, Washington University School of Medicine, Saint Louis, MO, USA. LA - eng GR - R21 AI139813/AI/NIAID NIH HHS/United States GR - HHSN272201400008C/AI/NIAID NIH HHS/United States GR - K08 GM129763/GM/NIGMS NIH HHS/United States GR - U01 AI141990/AI/NIAID NIH HHS/United States GR - HHSN272201400006C/AI/NIAID NIH HHS/United States GR - P30 CA091842/CA/NCI NIH HHS/United States GR - UL1 TR000448/TR/NCATS NIH HHS/United States GR - R35 GM126928/GM/NIGMS NIH HHS/United States GR - UL1 TR002345/TR/NCATS NIH HHS/United States GR - 75N93019C00051/AI/NIAID NIH HHS/United States GR - R01 AI121832/AI/NIAID NIH HHS/United States PT - Journal Article PT - Observational Study PT - Research Support, N.I.H., Extramural DEP - 20201209 PL - United States TA - Sci Adv JT - Science advances JID - 101653440 RN - 0 (Cytokines) SB - IM UOF - medRxiv. 2020 May 30;:. PMID: 32511543 MH - Adult MH - Aged MH - Aged, 80 and over MH - COVID-19/diagnosis/genetics/*immunology MH - Cells, Cultured MH - Cytokine Release Syndrome/genetics/*immunology/metabolism MH - Cytokines/genetics/*immunology/metabolism MH - Diagnosis, Differential MH - Female MH - Gene Expression Profiling/methods MH - Humans MH - Inflammation/genetics/*immunology MH - Influenza, Human/diagnosis/genetics/*immunology MH - Leukocytes, Mononuclear/immunology/metabolism MH - Male MH - Middle Aged MH - Prospective Studies MH - Young Adult PMC - PMC7725462 EDAT- 2020/11/15 06:00 MHDA- 2020/12/29 06:00 PMCR- 2020/12/09 CRDT- 2020/11/14 05:24 PHST- 2020/08/12 00:00 [received] PHST- 2020/10/26 00:00 [accepted] PHST- 2020/11/15 06:00 [pubmed] PHST- 2020/12/29 06:00 [medline] PHST- 2020/11/14 05:24 [entrez] PHST- 2020/12/09 00:00 [pmc-release] AID - sciadv.abe3024 [pii] AID - abe3024 [pii] AID - 10.1126/sciadv.abe3024 [doi] PST - epublish SO - Sci Adv. 2020 Dec 9;6(50):eabe3024. doi: 10.1126/sciadv.abe3024. Print 2020 Dec.