PMID- 33189800
OWN - NLM
STAT- MEDLINE
DCOM- 20210423
LR  - 20210423
IS  - 1879-176X (Electronic)
IS  - 0300-5712 (Linking)
VI  - 104
DP  - 2021 Jan
TI  - Zinc chloride inhibits lysine decarboxylase production from Eikenella corrodens 
      in vitro and its therapeutic implications.
PG  - 103533
LID - S0300-5712(20)30281-5 [pii]
LID - 10.1016/j.jdent.2020.103533 [doi]
AB  - OBJECTIVES: Dentifrices containing zinc reduce gingival inflammation and bleeding 
      better than control dentifrices (no zinc). How zinc might work is not understood. 
      We have shown that lysine decarboxylase (LdcE), an enzyme from Eikenella 
      corrodens, converts lysine to cadaverine in dental biofilms. The lack of lysine 
      impairs the dentally attached cell barrier to biofilm, causing biofilm products 
      to leak into junctional epithelium and stimulate inflammation. In year-old beagle 
      dogs, immunization with LdcE, induces antibodies that inhibit LdcE activity and 
      retard gingivitis development. We therefore examined whether a zinc-mediated loss 
      of LdcE activity could explain the beneficial effect of zinc dentifrices. 
      METHODS: We grew E. corrodens in modified tryptic soy broth with or without zinc 
      chloride, and extracted LdcE from the cell surface using a Potter Elvehjem 
      homogenizer. RESULTS: Up to 0.96 mM zinc chloride in the bacterial growth medium 
      did not change cell yield, but reduced the extracted protein content by 41% 
      (R(2) = 0.27, p < 0.05) and LdcE activity/mg extracted protein by 85% 
      (R(2) = 0.90, p < 0.001). In extracts from cells grown without zinc, 78 times 
      this zinc chloride concentration (73 mM) was required to reduce LdcE activity by 
      75%. CONCLUSIONS: Zinc ions inhibit the production of protein with LdcE activity 
      at E. corrodens cell surfaces. The zinc ions may attach to cysteine residues that 
      are unique to the N-terminal region of LdcE by interfering with the non-covalent 
      polypeptide assembly that produces enzyme activity. CLINICAL SIGNIFICANCE: Zinc 
      ion-mediated inhibition of LdcE assembly may provide a rationale for the improved 
      control of gingival inflammation by zinc dentifrices.
CI  - Copyright (c) 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Levine, Martin
AU  - Levine M
AD  - Department of Biochemistry and Molecular Biology, University of Oklahoma Health 
      Sciences Center, Oklahoma City, OK 73104, United States. Electronic address: 
      martin-levine@ouhsc.edu.
FAU - Collins, Lindsay M
AU  - Collins LM
AD  - Department of Biochemistry and Molecular Biology, University of Oklahoma Health 
      Sciences Center, Oklahoma City, OK 73104, United States.
FAU - Lohinai, Zsolt
AU  - Lohinai Z
AD  - Department of Conservative Dentistry, Semmelweis University, Budapest, Hungary.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201112
PL  - England
TA  - J Dent
JT  - Journal of dentistry
JID - 0354422
RN  - 0 (Chlorides)
RN  - 0 (Dentifrices)
RN  - 0 (Zinc Compounds)
RN  - 86Q357L16B (zinc chloride)
RN  - EC 4.1.1.- (Carboxy-Lyases)
RN  - EC 4.1.1.18 (lysine decarboxylase)
SB  - IM
MH  - Animals
MH  - *Carboxy-Lyases
MH  - Chlorides
MH  - *Dentifrices
MH  - Dogs
MH  - Eikenella corrodens
MH  - *Gingivitis/drug therapy
MH  - Zinc Compounds
OTO - NOTNLM
OT  - Biofilms
OT  - Dentifrice
OT  - Eikenella corrodens
OT  - Gingivitis
OT  - Lysine decarboxylase
OT  - Zinc
EDAT- 2020/11/16 06:00
MHDA- 2021/04/24 06:00
CRDT- 2020/11/15 20:25
PHST- 2020/05/26 00:00 [received]
PHST- 2020/08/26 00:00 [revised]
PHST- 2020/09/14 00:00 [accepted]
PHST- 2020/11/16 06:00 [pubmed]
PHST- 2021/04/24 06:00 [medline]
PHST- 2020/11/15 20:25 [entrez]
AID - S0300-5712(20)30281-5 [pii]
AID - 10.1016/j.jdent.2020.103533 [doi]
PST - ppublish
SO  - J Dent. 2021 Jan;104:103533. doi: 10.1016/j.jdent.2020.103533. Epub 2020 Nov 12.