PMID- 33191677
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210101
IS  - 2092-7355 (Print)
IS  - 2092-7363 (Electronic)
IS  - 2092-7355 (Linking)
VI  - 13
IP  - 1
DP  - 2021 Jan
TI  - Bacterial Microbiota-derived Extracellular Vesicles in Children With Allergic 
      Airway Diseases: Compositional and Functional Features.
PG  - 56-74
LID - 10.4168/aair.2021.13.1.56 [doi]
AB  - PURPOSE: Bacterial extracellular vesicles (EVs) play crucial roles in 
      bacteria-host interactions. Due to their cargo, EVs are considered fingerprints 
      of the parent cell, which are detectable in body fluids. We studied the 
      composition and function of bacterial microbiota-derived EVs genes in urine to 
      evaluate whether they have specific characteristics concerning allergic airway 
      disease. METHODS: Subjects were from elementary school surveys and classified 
      into 3 groups according to questionnaires and sensitization to aeroallergens: the 
      allergic airway group (AA, n = 16), atopic controls (AC, n = 7) and healthy 
      controls (HC, n = 26). The bacterial EVs were isolated from voided urine samples, 
      their nucleic acid was extracted for 16S ribosomal RNA pyrosequencing and then 
      characterized using alpha-diversity, beta-diversity, network analysis, intergroup 
      comparison of bacterial composition and predicted functions, and correlation with 
      total immunoglobulin E (IgE), eosinophils% and fractional exhaled NO. RESULTS: 
      The compositional alpha-diversity was the highest in AA, while functional alpha-diversity 
      was the highest in HC. AA had a distinct clustering with the least intersample 
      variation. Klebsiella, Haemophilus, members from Lachnospiraceae and 
      Ruminococcaceae, and the pathways of sphingolipid and glycerolipid metabolism, 
      and biosynthesis of peptidoglycan and lysine were the highest in AA and 
      positively correlated with total IgE or eosinophil%. Genetic information 
      processing function contributed to 48% of the intergroup variance and was the 
      highest in AA. Diaphorobacter, Acinetobacter, and the pathways of short-chain 
      fatty acids and anti-oxidants metabolism, lysine and xenobiotic degradation, and 
      lipopolysaccharide biosynthesis were the lowest in AA and negatively correlated 
      with total IgE or eosinophil%. The bacterial composition and function in AC were 
      closer to those in HC. The bacterial network was remarkably dense in HC. 
      CONCLUSIONS: The bacterial microbiota-derived EVs in urine possess characteristic 
      features in allergic airway disease with a remarkable correlation with total IgE 
      and eosinophil%. These findings suggest that they may play important roles in 
      allergic airway diseases.
CI  - Copyright (c) 2021 The Korean Academy of Asthma, Allergy and Clinical Immunology . 
      The Korean Academy of Pediatric Allergy and Respiratory Disease.
FAU - Samra, Mona Salem
AU  - Samra MS
AUID- ORCID: 0000-0001-6337-7049
AD  - Department of Pediatrics, Inha University College of Medicine, Incheon, Korea.
FAU - Lim, Dae Hyun
AU  - Lim DH
AUID- ORCID: 0000-0002-4558-3284
AD  - Department of Pediatrics, Inha University College of Medicine, Incheon, Korea.
FAU - Han, Man Yong
AU  - Han MY
AUID- ORCID: 0000-0002-9077-5779
AD  - Department of Pediatrics, CHA Bundang Medical Center, CHA University School of 
      Medicine, Seongnam, Korea.
FAU - Jee, Hye Mi
AU  - Jee HM
AUID- ORCID: 0000-0003-0128-065X
AD  - Department of Pediatrics, CHA Bundang Medical Center, CHA University School of 
      Medicine, Seongnam, Korea.
FAU - Kim, Yoon Keun
AU  - Kim YK
AD  - MD Healthcare Inc., Seoul, Korea.
FAU - Kim, Jeong Hee
AU  - Kim JH
AUID- ORCID: 0000-0002-7054-8552
AD  - Department of Pediatrics, Inha University College of Medicine, Incheon, Korea. 
      kimjhmd@inha.ac.kr.
LA  - eng
GR  - Korean Ministry of the Environment/Korea
GR  - Seongnam Atopy Project of Seongnam City/Korea
PT  - Journal Article
PL  - Korea (South)
TA  - Allergy Asthma Immunol Res
JT  - Allergy, asthma & immunology research
JID - 101518382
PMC - PMC7680829
OTO - NOTNLM
OT  - Microbiota
OT  - allergic rhinitis
OT  - asthma
OT  - bacteria
OT  - child
OT  - extracellular vesicles
OT  - hypersensitivity
OT  - metabolic networks and pathways
OT  - urine
COIS- There are no financial or other issues that might lead to a conflict of interest.
EDAT- 2020/11/17 06:00
MHDA- 2020/11/17 06:01
PMCR- 2021/01/01
CRDT- 2020/11/16 06:01
PHST- 2020/03/13 00:00 [received]
PHST- 2020/04/18 00:00 [revised]
PHST- 2020/04/21 00:00 [accepted]
PHST- 2020/11/16 06:01 [entrez]
PHST- 2020/11/17 06:00 [pubmed]
PHST- 2020/11/17 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 13.56 [pii]
AID - 10.4168/aair.2021.13.1.56 [doi]
PST - ppublish
SO  - Allergy Asthma Immunol Res. 2021 Jan;13(1):56-74. doi: 10.4168/aair.2021.13.1.56.