PMID- 33191681
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210101
IS  - 2092-7355 (Print)
IS  - 2092-7363 (Electronic)
IS  - 2092-7355 (Linking)
VI  - 13
IP  - 1
DP  - 2021 Jan
TI  - Cat-NPC2, a Newly Identified Allergen, With High Cross-Reactivity to Can f 7.
PG  - 122-140
LID - 10.4168/aair.2021.13.1.122 [doi]
AB  - PURPOSE: Pet-derived allergens are the common indoor inhalant allergens. Among 
      them, cat and dog allergens constitute more than 80% of animal allergic patients, 
      which greatly affect the quality-of-life of patients and increase the burden of 
      social health care. The aim of this study was to identify Cat-Niemann pick type 
      C2 (NPC2) protein, a homologue of Can f 7, as a new allergen. METHODS: Cat-NPC2 
      complementary DNA (cDNA) was cloned and optimized for amplification and 
      expression in Escherichia coli. Then, recombinant Cat-NPC2 (rCat-NPC2) was 
      purified by Ni(2+) affinity chromatography. The allergenicity was assessed by 
      enzyme-linked immunosorbent assay (ELISA), western blot and basophil activation 
      test (BAT). Based on the sequence similarity, the cross-reactivity between 
      Cat-NPC2 and Can f 7 was investigated by inhibition ELISA. Circular dichroism 
      spectroscopy and homology modeling were used to characterize the structure of 
      Cat-NPC2. RESULTS: The cDNA sequence of Cat-NPC2 was cloned with a 450-bp open 
      reading frame coding for 149 amino acids (GenBank MN_737596). The 
      condon-optimized NPC2 gene was subcloned and expressed in E. coli with a 
      molecular weight of 18.9 kDa. The native Cat-NPC2 was detected in cat dander 
      extracts. The allergenicity determined by ELISA, western blot and BAT suggested 
      at least 14.5% cat-allergic patients displayed high specific immunoglobulin E 
      (IgE) recognition of Cat-NPC2. The predicted structure of Cat-NPC2 was found to 
      consist of 7 beta-strands arranged in 2 beta-sheets. An ELISA based assay showed that 
      rCat-NPC2 bound to cholesterol in a dose dependent manner. Based on the structure 
      and sequence similarities, IgE cross-reactivity was demonstrated between Cat-NPC2 
      and Can f 7/Der f 2. CONCLUSIONS: In the study, a novel cat allergen, belonging 
      to the NPC2 protein family, was identified and characterized at both molecular 
      and immunological levels. The study will offer a deeper understanding of cat 
      allergens and improve a component-resolved diagnosis in pet allergy.
CI  - Copyright (c) 2021 The Korean Academy of Asthma, Allergy and Clinical Immunology . 
      The Korean Academy of Pediatric Allergy and Respiratory Disease.
FAU - Zhu, Dan Xuan
AU  - Zhu DX
AD  - Women & Children Central Laboratory, The First Affiliated Hospital of Nanjing 
      Medical University, Nanjing, China.
FAU - Li, Lin
AU  - Li L
AD  - Department of Emergency Medicine, The First Affiliated Hospital of Nanjing 
      Medical University, Nanjing, China.
FAU - Xu, Zhi Qiang
AU  - Xu ZQ
AD  - Research Division of Clinical Pharmacology, The First Affiliated Hospital of 
      Nanjing Medical University, Nanjing, China.
FAU - Zhang, Cheng
AU  - Zhang C
AD  - Women & Children Central Laboratory, The First Affiliated Hospital of Nanjing 
      Medical University, Nanjing, China.
FAU - Zhang, Jin Song
AU  - Zhang JS
AD  - Department of Emergency Medicine, The First Affiliated Hospital of Nanjing 
      Medical University, Nanjing, China. zhangjso@sina.com.
FAU - Sun, Jin Lyu
AU  - Sun JL
AD  - Department of Allergy, Peking Union Medical College Hospital, Chinese Academy of 
      Medical Sciences, Beijing, China. sunjinlv@pumch.cn.
FAU - Wei, Ji Fu
AU  - Wei JF
AUID- ORCID: 0000-0001-6412-3251
AD  - Research Division of Clinical Pharmacology, The First Affiliated Hospital of 
      Nanjing Medical University, Nanjing, China. weijifu@hotmail.com.
LA  - eng
GR  - 81871265/NSFC/National Natural Science Foundation of China/China
GR  - 81771725/NSFC/National Natural Science Foundation of China/China
GR  - U1832212/NSFC/National Natural Science Foundation of China/China
GR  - 2016-I2M-1003/CAMS Innovation Fund for Medical Sciences/China
GR  - PAPD/Priority Academic Program Development of Jiangsu Higher Education 
      Institutions/China
PT  - Journal Article
PL  - Korea (South)
TA  - Allergy Asthma Immunol Res
JT  - Allergy, asthma & immunology research
JID - 101518382
PMC - PMC7680833
OTO - NOTNLM
OT  - Allergy
OT  - Felis domesticus
OT  - NPC2 protein
OT  - allergens
OT  - cross reactions
COIS- There are no financial or other issues that might lead to conflict of interest.
EDAT- 2020/11/17 06:00
MHDA- 2020/11/17 06:01
PMCR- 2021/01/01
CRDT- 2020/11/16 06:01
PHST- 2020/02/26 00:00 [received]
PHST- 2020/05/21 00:00 [revised]
PHST- 2020/06/15 00:00 [accepted]
PHST- 2020/11/16 06:01 [entrez]
PHST- 2020/11/17 06:00 [pubmed]
PHST- 2020/11/17 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 13.122 [pii]
AID - 10.4168/aair.2021.13.1.122 [doi]
PST - ppublish
SO  - Allergy Asthma Immunol Res. 2021 Jan;13(1):122-140. doi: 
      10.4168/aair.2021.13.1.122.