PMID- 33191683
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210101
IS  - 2092-7355 (Print)
IS  - 2092-7363 (Electronic)
IS  - 2092-7355 (Linking)
VI  - 13
IP  - 1
DP  - 2021 Jan
TI  - Expression in Escherichia coli and Purification of Folded rDer p 20, the Arginine 
      Kinase From Dermatophagoides pteronyssinus: A Possible Biomarker for Allergic 
      Asthma.
PG  - 154-163
LID - 10.4168/aair.2021.13.1.154 [doi]
AB  - Arginine kinase (AK) was first identified as an allergen in the Indian-meal moth 
      and subsequently shown to occur as allergen in various invertebrates and 
      shellfish. The cDNA coding for AK from the house dust mite (HDM) species 
      Dermatophagoides pteronyssinus, Der p 20, has been isolated, but no recombinant 
      Der p 20 (rDer p 20) allergen has been produced and characterized so far. We 
      report the expression of Der p 20 as recombinant protein in Escherichia coli. 
      rDer p 20 was purified and shown to be a monomeric, folded protein by size 
      exclusion chromatography and circular dichroism spectroscopy, respectively. Using 
      AK-specific antibodies, Der p 20 was found to occur mainly in HDM bodies, but not 
      in fecal particles. Thirty percent of clinically well-characterized HDM allergic 
      patients (n = 98) whose immunoglobulin E (IgE) reactivity profiles had been 
      determined with an extensive panel of purified HDM allergens (Der f 1, 2; Der p 
      1, 2, 4, 5, 7, 10, 11, 14, 15, 18, 21, 23 and 37) showed IgE reactivity to Der p 
      20. IgE reactivity to Der p 20 was more frequently associated with lung symptoms. 
      AKs were detected in several invertebrates with specific antibodies and Der p 20 
      showed IgE cross-reactivity with AK from shrimp (Litopenaeus vannamei). Thus, Der 
      p 20 is a cross-reactive HDM allergen and may serve as a diagnostic marker for 
      HDM-induced lung symptoms such as asthma.
CI  - Copyright (c) 2021 The Korean Academy of Asthma, Allergy and Clinical Immunology . 
      The Korean Academy of Pediatric Allergy and Respiratory Disease.
FAU - Sarzsinszky, Eszter
AU  - Sarzsinszky E
AUID- ORCID: 0000-0001-8109-312X
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Lupinek, Christian
AU  - Lupinek C
AUID- ORCID: 0000-0002-8612-8245
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria. christian.lupinek@meduniwien.ac.at.
FAU - Vrtala, Susanne
AU  - Vrtala S
AUID- ORCID: 0000-0003-4250-8243
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Huang, Huey Jy
AU  - Huang HJ
AUID- ORCID: 0000-0001-5464-0132
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Hofer, Gerhard
AU  - Hofer G
AUID- ORCID: 0000-0002-9248-6989
AD  - Institute of Molecular Biosciences, BioTechMed Graz, University of Graz, Graz, 
      Austria.
FAU - Keller, Walter
AU  - Keller W
AUID- ORCID: 0000-0002-2261-958X
AD  - Institute of Molecular Biosciences, BioTechMed Graz, University of Graz, Graz, 
      Austria.
FAU - Chen, Kuan Wei
AU  - Chen KW
AUID- ORCID: 0000-0001-5899-5766
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria.
AD  - OncoGen Center, County Clinical Emergency Hospital 'Pius Branzeu', Timisoara, 
      Romania.
FAU - Panaitescu, Carmen Bunu
AU  - Panaitescu CB
AUID- ORCID: 0000-0001-8749-9972
AD  - OncoGen Center, County Clinical Emergency Hospital 'Pius Branzeu', Timisoara, 
      Romania.
AD  - Department of Functional Sciences, Victor Babes University of Medicine and 
      Pharmacy, Timisoara, Romania.
FAU - Resch-Marat, Yvonne
AU  - Resch-Marat Y
AUID- ORCID: 0000-0002-7933-2547
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Zieglmayer, Petra
AU  - Zieglmayer P
AUID- ORCID: 0000-0001-5432-9459
AD  - Vienna Challenge Chamber, Allergy Center Vienna West, Vienna, Austria.
FAU - Zieglmayer, Rene
AU  - Zieglmayer R
AD  - Vienna Challenge Chamber, Allergy Center Vienna West, Vienna, Austria.
FAU - Lemell, Patrick
AU  - Lemell P
AUID- ORCID: 0000-0003-0262-4047
AD  - Vienna Challenge Chamber, Allergy Center Vienna West, Vienna, Austria.
FAU - Horak, Friedrich
AU  - Horak F
AD  - Vienna Challenge Chamber, Allergy Center Vienna West, Vienna, Austria.
FAU - Duchene, Michael
AU  - Duchene M
AUID- ORCID: 0000-0003-1232-3600
AD  - Institute of Specific Prophylaxis and Tropical Medicine, Center for 
      Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 
      Vienna, Austria.
FAU - Valenta, Rudolf
AU  - Valenta R
AUID- ORCID: 0000-0001-5944-3365
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria.
AD  - NRC Institute of Immunology FMBA of Russia, Moscow, Russia.
AD  - Department of Clinical Immunology and Allergy, Laboratory of Immunopathology, 
      Sechenov First Moscow State Medical University, Moscow, Russia.
AD  - Karl Landsteiner University of Health Sciences, Krems, Austria.
LA  - eng
GR  - Country of Lower Austria/Austria
GR  - HVD Life Science/Austria
GR  - 14.W03.31.0024/Megagrant of the Government of the Russian Federation/Russian 
      Federation
PT  - Journal Article
PL  - Korea (South)
TA  - Allergy Asthma Immunol Res
JT  - Allergy, asthma & immunology research
JID - 101518382
PMC - PMC7680834
OTO - NOTNLM
OT  - Der p 20 arginine kinase
OT  - Escherichia coli
OT  - IgE
OT  - allergic asthma
OT  - allergy and immunology
OT  - allergy diagnosis
OT  - biomarker
OT  - house dust mites
OT  - recombinant allergen
COIS- RV has received research grants from HVD Life Science, Vienna Austria and 
      Viravaxx, Vienna, Austria and serves as a consultant forViravaxx. The other 
      authors declare that they have no conflicts of interest.
EDAT- 2020/11/17 06:00
MHDA- 2020/11/17 06:01
PMCR- 2021/01/01
CRDT- 2020/11/16 06:01
PHST- 2019/12/02 00:00 [received]
PHST- 2020/04/26 00:00 [revised]
PHST- 2020/05/10 00:00 [accepted]
PHST- 2020/11/16 06:01 [entrez]
PHST- 2020/11/17 06:00 [pubmed]
PHST- 2020/11/17 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 13.154 [pii]
AID - 10.4168/aair.2021.13.1.154 [doi]
PST - ppublish
SO  - Allergy Asthma Immunol Res. 2021 Jan;13(1):154-163. doi: 
      10.4168/aair.2021.13.1.154.