PMID- 33191698
OWN - NLM
STAT- MEDLINE
DCOM- 20201117
LR  - 20220418
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 34
IP  - 11
DP  - 2020 Nov 15
TI  - [Effect of glycoside of short-horned epimedium Herb on the expressions of 
      transforming growth factor beta (1) and basic fibroblast growth factor induced 
      membrane by Masquelet technology].
PG  - 1405-1409
LID - 10.7507/1002-1892.201905060 [doi]
AB  - OBJECTIVE: To observe the expressions of transforming growth factor beta (1) (TGF-beta 
      (1)) and basic fibroblast growth factor (bFGF) induced membrane by Masquelet 
      technique in rats treated with glycoside of short-horned epimedium Herb, and to 
      explore the effect of glycoside of short-horned epimedium Herb on Masquelet 
      induced membrane. METHODS: Sixty 3-month-old male Wistar rats were randomly 
      divided into 3 groups with 20 rats in each group; a tibial bone defect (6 mm in 
      length) model was established. The blank group (group A) was not treated; the 
      control group (group B) and the experimental group (group C) were filled with 
      vancomycin antibiotic bone cement in the drawing stage, and the bone cement was 
      completely solidified. Group C was given perfused flavonoids glycoside of 
      short-horned epimedium Herb (10 mumol/L) by gavage once a day (0.3 mL) from 1 day 
      after operation, and groups A and B were given the same amount of normal saline 
      by gavage. After operation, the recovery and wound healing of experimental 
      animals were observed; at 4 weeks after operation, X-ray film was taken to 
      observe the recovery of bone defect of proximal tibia; at 6 weeks after 
      operation, the bone defect was observed, and the morphological changes and 
      vascularization degree of granulation tissue and induction membrane tissue were 
      observed; the expressions of TGF-beta (1) and bFGF were observed by 
      immunohistochemistry staining and ELISA detection. RESULTS: The bone defect 
      models of the 3 groups were established successfully, and there was no 
      abnormality after operation. The incisions healed by first intention after 
      operation. At 4 weeks after operation, X-ray films of proximal tibial defect 
      showed that there was obvious space in group A, while bone cement was filled and 
      Kirschner wire fixation was good in groups B and C. At 6 weeks after operation, 
      the gross observation showed that the granulation tissue was filled in the defect 
      area in group A; transparent membrane was formed in groups B and C, and 
      microvessels were seen in some areas, and the microvessels in group C were 
      significantly more than those in group B. Immunohistochemical staining showed 
      that the expressions of TGF-beta (1) and bFGF were negative in group A, but they 
      were expressed in groups B and C, and the expressions of TGF-beta (1) and bFGF in 
      group B were significantly less than those in group C. ELISA detection showed 
      that the expressions of TGF-beta (1) and bFGF in group C were significantly higher 
      than those in groups A and B ( P<0.05), but there was no significant difference 
      between groups A and B ( P>0.05). CONCLUSION: Glycoside of short-horned epimedium 
      Herb can significantly increase the expressions of TGF-beta (1) and bFGF, accelerate 
      the process of osteogenesis, and contribute to bone shaping and reconstruction.
FAU - Ding, Qiang
AU  - Ding Q
AD  - Department of Osteonecrosis, Luoyang Orthopedic-Traumatological Hospital of Henan 
      Province (Henan Provincial Orthopedic Hospital), Luoyang Henan, 471002, 
      P.R.China.
FAU - Sun, Nan
AU  - Sun N
AD  - Department of Osteonecrosis, Luoyang Orthopedic-Traumatological Hospital of Henan 
      Province (Henan Provincial Orthopedic Hospital), Luoyang Henan, 471002, 
      P.R.China.
FAU - Wang, Xiaohui
AU  - Wang X
AD  - Department of Osteonecrosis, Luoyang Orthopedic-Traumatological Hospital of Henan 
      Province (Henan Provincial Orthopedic Hospital), Luoyang Henan, 471002, 
      P.R.China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Glycosides)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Animals
MH  - *Epimedium
MH  - *Fibroblast Growth Factor 2
MH  - Glycosides/pharmacology
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Transforming Growth Factor beta
MH  - Transforming Growth Factor beta1
MH  - Wound Healing
PMC - PMC8171696
OTO - NOTNLM
OT  - Bone defect
OT  - Masquelet technique
OT  - basic fibroblast growth factor
OT  - glycoside of short-horned epimedium Herb
OT  - osteogenic activity
OT  - transforming growth factor beta1
COIS- 利益冲突：所有作者声明，在课题研究和文章撰写过程中不存在利益冲突。课题经费支持没有影响文章观点和对研究数据客观结果的统计分析及其报道。
EDAT- 2020/11/17 06:00
MHDA- 2020/11/18 06:00
PMCR- 2020/11/01
CRDT- 2020/11/16 06:01
PHST- 2020/11/16 06:01 [entrez]
PHST- 2020/11/17 06:00 [pubmed]
PHST- 2020/11/18 06:00 [medline]
PHST- 2020/11/01 00:00 [pmc-release]
AID - zgxfcjwkzz-34-11-1405 [pii]
AID - 10.7507/1002-1892.201905060 [doi]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2020 Nov 15;34(11):1405-1409. doi: 
      10.7507/1002-1892.201905060.