PMID- 33192315
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201117
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 14
DP  - 2020
TI  - Genetically Encoded Calcium Indicators Can Impair Dendrite Growth of Cortical 
      Neurons.
PG  - 570596
LID - 10.3389/fncel.2020.570596 [doi]
LID - 570596
AB  - A battery of genetically encoded calcium indicators (GECIs) with different 
      binding kinetics and calcium affinities was developed over the recent years to 
      permit long-term calcium imaging. GECIs are calcium buffers and therefore, 
      expression of GECIs may interfere with calcium homeostasis and signaling pathways 
      important for neuronal differentiation and survival. Our objective was to 
      investigate if the biolistically induced expression of five commonly used GECIs 
      at two postnatal time points (days 14 and 22-25) could affect the morphological 
      maturation of cortical neurons in organotypic slice cultures of rat visual 
      cortex. Expression of GCaMP3 in both time windows, and of GCaMP5G and TN-XXL in 
      the later time window impaired apical and /or basal dendrite growth of pyramidal 
      neurons. With time, the proportion of GECI transfectants with nuclear filling 
      increased, but an only prolonged expression of TN-XXL caused higher levels of 
      neurodegeneration. In multipolar interneurons, only GCaMP3 evoked a transient 
      growth delay during the early time window. GCaMP6m and GCaMP6m-X(C) were quite 
      "neuron-friendly." Since growth-impaired neurons might not have the physiological 
      responses typical of age-matched wildtype neurons the results obtained after 
      prolonged developmental expression of certain GECIs might need to be interpreted 
      with caution.
CI  - Copyright (c) 2020 Gasterstadt, Jack, Stahlhut, Rennau, Gonda and Wahle.
FAU - Gasterstadt, Ina
AU  - Gasterstadt I
AD  - Developmental Neurobiology, Faculty of Biology and Biotechnology, Ruhr University 
      Bochum, Bochum, Germany.
FAU - Jack, Alexander
AU  - Jack A
AD  - Developmental Neurobiology, Faculty of Biology and Biotechnology, Ruhr University 
      Bochum, Bochum, Germany.
FAU - Stahlhut, Tobias
AU  - Stahlhut T
AD  - Developmental Neurobiology, Faculty of Biology and Biotechnology, Ruhr University 
      Bochum, Bochum, Germany.
FAU - Rennau, Lisa-Marie
AU  - Rennau LM
AD  - Developmental Neurobiology, Faculty of Biology and Biotechnology, Ruhr University 
      Bochum, Bochum, Germany.
FAU - Gonda, Steffen
AU  - Gonda S
AD  - Developmental Neurobiology, Faculty of Biology and Biotechnology, Ruhr University 
      Bochum, Bochum, Germany.
FAU - Wahle, Petra
AU  - Wahle P
AD  - Developmental Neurobiology, Faculty of Biology and Biotechnology, Ruhr University 
      Bochum, Bochum, Germany.
LA  - eng
PT  - Journal Article
DEP - 20201020
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
PMC - PMC7606991
OTO - NOTNLM
OT  - Fe65
OT  - calcium imaging
OT  - cell death
OT  - dendritic injury
OT  - dendritogenesis
OT  - neurite growth
OT  - postnatal development
EDAT- 2020/11/17 06:00
MHDA- 2020/11/17 06:01
PMCR- 2020/01/01
CRDT- 2020/11/16 08:44
PHST- 2020/06/08 00:00 [received]
PHST- 2020/08/31 00:00 [accepted]
PHST- 2020/11/16 08:44 [entrez]
PHST- 2020/11/17 06:00 [pubmed]
PHST- 2020/11/17 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2020.570596 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2020 Oct 20;14:570596. doi: 10.3389/fncel.2020.570596. 
      eCollection 2020.