PMID- 33194340
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220418
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 8
DP  - 2020
TI  - Overexpression of KIAA1199 is an independent prognostic marker in laryngeal 
      squamous cell carcinoma.
PG  - e9637
LID - 10.7717/peerj.9637 [doi]
LID - e9637
AB  - BACKGROUND: KIAA1199 is a recently identified novel gene that is upregulated in 
      various human cancers with poor survival, but its role and the underlying 
      mechanisms in laryngeal squamous cell carcinoma (LSCC) remain unknown. Here, we 
      collected tissues from 105 cases of LSCC to investigate the relationships between 
      KIAA1199 protein expression and clinical factors. METHODS: Western blotting and 
      real-time quantitative PCR (RT-PCR) were used for detect the protein and mRNA 
      expression of KIAA1199 in LSCC tissue. Immunohistochemistry (IHC) staining was 
      used to detect the expression of KIAA1199. Patient clinical information, for 
      instance sex, age, pathological differentiation, clinical region, T stage, N 
      stage, clinical stage, operation type, neck lymph dissection, smoking status, and 
      drinking status were recorded. Kaplan-Meier survival analysis and Cox analysis 
      were applied to identify the relationship between KIAA1199 and LSCC. RESULTS: 
      Western blotting results showed KIAA1199 protein was significantly higher in 
      tumor tissues vs. adjacent non-cancerous tissues (0.9385 +/- 0.1363 vs. 1.838 +/- 
      0.3209, P = 0.04). The KIAA1199 mRNA expression was considerably higher in tumor 
      tissues (P < 0.001) than in adjacent non-cancerous tissues by RT-PCR. IHC results 
      showed up-regulated KIAA1199 expression was related with some severe 
      clinicopathological parameters: pathologic differentiation (P = 0.002), T stage 
      (P < 0.001), N stage (P < 0.001), clinical stage (P < 0.001), survival time (P = 
      0.008) and survival status (P < 0.001). Kaplan-Meier survival analysis showed 
      that patients with high KIAA1199 protein expression had poor overall survival 
      (OS) (P < 0.05). Cox analysis suggested that the KIAA1199 protein expression 
      constituted an independent prognostic marker for LSCC patients (P < 0.001). 
      CONCLUSION: Our findings revealed that KIAA1199 protein expression may be used to 
      predict LSCC patient outcome.
CI  - (c) 2020 Huang et al.
FAU - Huang, Meixiang
AU  - Huang M
AD  - Department of Otolaryngology Head and Neck Surgery, The Third Xiangya Hospital, 
      Central South University, Changsha, Hunan, China.
FAU - Liao, Feifei
AU  - Liao F
AD  - Department of Pathology, The Third Xiangya Hospital, Central South University, 
      Changsha, Hunan, Chian.
FAU - Song, Yexun
AU  - Song Y
AD  - Department of Otolaryngology Head and Neck Surgery, The Third Xiangya Hospital, 
      Central South University, Changsha, Hunan, China.
FAU - Zuo, Gang
AU  - Zuo G
AD  - Ministry of Education (Central South University), Key Laboratory of Metallogenic 
      Prediction of Nonferrous Metals and Geological Environment Monitoring, Changsha, 
      Hunan, China.
FAU - Tan, Guolin
AU  - Tan G
AD  - Department of Otolaryngology Head and Neck Surgery, The Third Xiangya Hospital, 
      Central South University, Changsha, Hunan, China.
FAU - Chu, Ling
AU  - Chu L
AD  - Department of Pathology, The Third Xiangya Hospital, Central South University, 
      Changsha, Hunan, Chian.
FAU - Wang, Tiansheng
AU  - Wang T
AD  - Department of Otolaryngology Head and Neck Surgery, The Third Xiangya Hospital, 
      Central South University, Changsha, Hunan, China.
LA  - eng
PT  - Journal Article
DEP - 20200907
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
PMC - PMC7482636
OTO - NOTNLM
OT  - CEMIP
OT  - KIAA1199
OT  - Laryngeal squamous cell carcinoma
OT  - Prognostic marker
COIS- The authors declare that they have no competing interests.
EDAT- 2020/11/17 06:00
MHDA- 2020/11/17 06:01
PMCR- 2020/09/07
CRDT- 2020/11/16 08:51
PHST- 2020/01/30 00:00 [received]
PHST- 2020/07/09 00:00 [accepted]
PHST- 2020/11/16 08:51 [entrez]
PHST- 2020/11/17 06:00 [pubmed]
PHST- 2020/11/17 06:01 [medline]
PHST- 2020/09/07 00:00 [pmc-release]
AID - 9637 [pii]
AID - 10.7717/peerj.9637 [doi]
PST - epublish
SO  - PeerJ. 2020 Sep 7;8:e9637. doi: 10.7717/peerj.9637. eCollection 2020.