PMID- 33194433
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201117
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 8
DP  - 2020
TI  - Cytological and molecular characterizations of a novel 2A nullisomic line derived 
      from a widely-grown wheat cultivar Zhoumai 18 conferring male sterility.
PG  - e10275
LID - 10.7717/peerj.10275 [doi]
LID - e10275
AB  - A dwarf, multi-pistil and male sterile dms mutant was previously reported by us. 
      However, the genetic changes in this dms are unclear. To examine the genetic 
      changes, single nucleotide polymorphism (SNP) association, chromosome counting, 
      and high-resolution chromosome fluorescence in situ hybridization (FISH) 
      techniques were employed. By comparing tall plants (T) with dwarf plants (D) in 
      the offspring of dms mutant plants, SNP association analysis indicated that most 
      SNPs were on chromosome 2A. There were three types in offspring of dms plants, 
      with 42, 41 and 40 chromosomes respectively. High-resolution chromosome painting 
      analysis demonstrated that T plants had all 42 wheat chromosomes; the medium 
      plants (M) had 41 chromosomes, lacking one chromosome 2A; while D plants had 40 
      wheat chromosomes, and lacked both 2A chromosomes. These data demonstrated that 
      dms resulted from a loss of chromosome 2A. We identified 23 genes on chromosome 
      2A which might be involved in the development of stamens or pollen grains. These 
      results lay a solid foundation for further analysis of the molecular mechanisms 
      of wheat male sterility. Because D plants can be used as a female parent to cross 
      with other wheat genotypes, dms is a unique germplasm for any functional study of 
      chromosome 2A and wheat breeding specifically targeting genes on 2A.
CI  - (c)2020 Jiao et al.
FAU - Jiao, Zhixin
AU  - Jiao Z
AD  - Henan Agricultural University, National Centre of Engineering and Technological 
      Research for Wheat / National Key Laboratory of Wheat and Maize Crop Science, 
      Zhengzhou, Henan, China.
FAU - Zhu, Xinxin
AU  - Zhu X
AD  - Henan Agricultural University, National Centre of Engineering and Technological 
      Research for Wheat / National Key Laboratory of Wheat and Maize Crop Science, 
      Zhengzhou, Henan, China.
FAU - Li, Huijuan
AU  - Li H
AD  - Henan Agricultural University, National Centre of Engineering and Technological 
      Research for Wheat / National Key Laboratory of Wheat and Maize Crop Science, 
      Zhengzhou, Henan, China.
FAU - Liu, Zhitao
AU  - Liu Z
AD  - Nanjing Agricultural University, State key Laboratory of Crop Genetics and 
      Germplasm Enhancement, Nanjing, Jiangsu, China.
AD  - Sichuan Academy of Agricultural Sciences, Crop Research Institue, Chengdu, 
      Sichuan, China.
FAU - Huang, Xinyi
AU  - Huang X
AD  - Nanjing Agricultural University, State key Laboratory of Crop Genetics and 
      Germplasm Enhancement, Nanjing, Jiangsu, China.
FAU - Wu, Nan
AU  - Wu N
AD  - Nanjing Agricultural University, State key Laboratory of Crop Genetics and 
      Germplasm Enhancement, Nanjing, Jiangsu, China.
FAU - An, Junhang
AU  - An J
AD  - Henan Agricultural University, National Centre of Engineering and Technological 
      Research for Wheat / National Key Laboratory of Wheat and Maize Crop Science, 
      Zhengzhou, Henan, China.
FAU - Li, Junchang
AU  - Li J
AD  - Henan Agricultural University, National Centre of Engineering and Technological 
      Research for Wheat / National Key Laboratory of Wheat and Maize Crop Science, 
      Zhengzhou, Henan, China.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Henan Agricultural University, National Centre of Engineering and Technological 
      Research for Wheat / National Key Laboratory of Wheat and Maize Crop Science, 
      Zhengzhou, Henan, China.
FAU - Jiang, Yumei
AU  - Jiang Y
AD  - Henan Agricultural University, National Centre of Engineering and Technological 
      Research for Wheat / National Key Laboratory of Wheat and Maize Crop Science, 
      Zhengzhou, Henan, China.
FAU - Li, Qiaoyun
AU  - Li Q
AD  - Henan Agricultural University, National Centre of Engineering and Technological 
      Research for Wheat / National Key Laboratory of Wheat and Maize Crop Science, 
      Zhengzhou, Henan, China.
FAU - Qi, Zengjun
AU  - Qi Z
AD  - Nanjing Agricultural University, State key Laboratory of Crop Genetics and 
      Germplasm Enhancement, Nanjing, Jiangsu, China.
FAU - Niu, Jishan
AU  - Niu J
AD  - Henan Agricultural University, National Centre of Engineering and Technological 
      Research for Wheat / National Key Laboratory of Wheat and Maize Crop Science, 
      Zhengzhou, Henan, China.
LA  - eng
PT  - Journal Article
DEP - 20201030
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
PMC - PMC7605228
OTO - NOTNLM
OT  - Chromosome
OT  - Nullisomic line
OT  - Pollen development
OT  - Wheat (Triticum aestivum L.)
OT  - dms
COIS- The authors declare there are no competing interests.
EDAT- 2020/11/17 06:00
MHDA- 2020/11/17 06:01
PMCR- 2020/10/30
CRDT- 2020/11/16 08:51
PHST- 2020/06/19 00:00 [received]
PHST- 2020/10/08 00:00 [accepted]
PHST- 2020/11/16 08:51 [entrez]
PHST- 2020/11/17 06:00 [pubmed]
PHST- 2020/11/17 06:01 [medline]
PHST- 2020/10/30 00:00 [pmc-release]
AID - 10275 [pii]
AID - 10.7717/peerj.10275 [doi]
PST - epublish
SO  - PeerJ. 2020 Oct 30;8:e10275. doi: 10.7717/peerj.10275. eCollection 2020.