PMID- 33195213
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240330
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 8
DP  - 2020
TI  - The Planar Polarity Component VANGL2 Is a Key Regulator of Mechanosignaling.
PG  - 577201
LID - 10.3389/fcell.2020.577201 [doi]
LID - 577201
AB  - VANGL2 is a component of the planar cell polarity (PCP) pathway, which regulates 
      tissue polarity and patterning. The Vangl2 (Lp) mutation causes lung branching 
      defects due to dysfunctional actomyosin-driven morphogenesis. Since the 
      actomyosin network regulates cell mechanics, we speculated that mechanosignaling 
      could be impaired when VANGL2 is disrupted. Here, we used live-imaging of 
      precision-cut lung slices (PCLS) from Vangl2 (Lp/+) mice to determine that 
      alveologenesis is attenuated as a result of impaired epithelial cell migration. 
      Vangl2 (Lp/+) tracheal epithelial cells (TECs) and alveolar epithelial cells 
      (AECs) exhibited highly disrupted actomyosin networks and focal adhesions (FAs). 
      Functional assessment of cellular forces confirmed impaired traction force 
      generation in Vangl2 (Lp/+) TECs. YAP signaling in Vangl2 (Lp) airway epithelium 
      was reduced, consistent with a role for VANGL2 in mechanotransduction. 
      Furthermore, activation of RhoA signaling restored actomyosin organization in 
      Vangl2 (Lp/+) , confirming RhoA as an effector of VANGL2. This study identifies a 
      pivotal role for VANGL2 in mechanosignaling, which underlies the key role of the 
      PCP pathway in tissue morphogenesis.
CI  - Copyright (c) 2020 Cheong, Akram, Matellan, Kim, Gaboriau, Hind, del Rio Hernandez, 
      Griffiths and Dean.
FAU - Cheong, Sek-Shir
AU  - Cheong SS
AD  - National Heart and Lung Institute, Imperial College London, London, United 
      Kingdom.
FAU - Akram, Khondoker M
AU  - Akram KM
AD  - National Heart and Lung Institute, Imperial College London, London, United 
      Kingdom.
FAU - Matellan, Carlos
AU  - Matellan C
AD  - Cellular and Molecular Biomechanics Laboratory, Department of Bioengineering, 
      Imperial College London, London, United Kingdom.
FAU - Kim, Sally Yunsun
AU  - Kim SY
AD  - National Heart and Lung Institute, Imperial College London, London, United 
      Kingdom.
FAU - Gaboriau, David C A
AU  - Gaboriau DCA
AD  - Facility for Imaging by Light Microscopy, National Heart and Lung Institute, 
      Faculty of Medicine, Imperial College London, London, United Kingdom.
FAU - Hind, Matthew
AU  - Hind M
AD  - National Heart and Lung Institute, Imperial College London, London, United 
      Kingdom.
AD  - National Institute for Health Research, Respiratory Biomedical Research Unit, 
      Royal Brompton & Harefield NHS Foundation Trust, London, United Kingdom.
FAU - Del Rio Hernandez, Armando E
AU  - Del Rio Hernandez AE
AD  - Cellular and Molecular Biomechanics Laboratory, Department of Bioengineering, 
      Imperial College London, London, United Kingdom.
FAU - Griffiths, Mark
AU  - Griffiths M
AD  - National Heart and Lung Institute, Imperial College London, London, United 
      Kingdom.
AD  - Peri-Operative Medicine Department, St Bartholomew's Hospital, London, United 
      Kingdom.
FAU - Dean, Charlotte H
AU  - Dean CH
AD  - National Heart and Lung Institute, Imperial College London, London, United 
      Kingdom.
AD  - MRC Harwell Institute, Harwell Campus, Oxfordshire, United Kingdom.
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
DEP - 20201029
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC7658195
OTO - NOTNLM
OT  - RhoA
OT  - VANGL2
OT  - YAP signaling
OT  - cell migration
OT  - focal adhesion
OT  - mechanosignaling
OT  - planar cell polarity
OT  - traction force
EDAT- 2020/11/17 06:00
MHDA- 2020/11/17 06:01
PMCR- 2020/01/01
CRDT- 2020/11/16 08:54
PHST- 2020/06/28 00:00 [received]
PHST- 2020/10/08 00:00 [accepted]
PHST- 2020/11/16 08:54 [entrez]
PHST- 2020/11/17 06:00 [pubmed]
PHST- 2020/11/17 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fcell.2020.577201 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2020 Oct 29;8:577201. doi: 10.3389/fcell.2020.577201. 
      eCollection 2020.