PMID- 33195513
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201117
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 7
DP  - 2020
TI  - Development of a Novel Double Antibody Sandwich Quantitative Enzyme-Linked 
      Immunosorbent Assay for Detection of Porcine Epidemic Diarrhea Virus Antigen.
PG  - 540248
LID - 10.3389/fvets.2020.540248 [doi]
LID - 540248
AB  - Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea and dehydration in 
      sucking piglets with a high mortality rate. Here, we developed a double antibody 
      sandwich quantitative enzyme-linked immunosorbent assay (DAS-qELISA) for 
      detection of PEDV using a specific monoclonal antibody against PEDV N protein and 
      anti-PEDV rabbit serum. Using DAS-qELISA, the detection limit of recombinant PEDV 
      N protein and virus titer were approximately 1 mug/L and 10(2.0) TCID(50)/ml, 
      respectively. A total of 90 intestinal and 237 fecal samples were then screened 
      for the presence of PEDV using DAS-qELISA and reverse transcriptase PCR (RT-PCR). 
      DAS-qELISA had a high specificity of 98.1% and sensitivity of 93.5%. The accuracy 
      rate between DAS-qELISA and RT-PCR was 95.7%. More importantly, the viral antigen 
      concentrations remained unchanged before and after one inactivated vaccine 
      preparation by using the DAS-qELISA. These results suggest DAS-qELISA could be 
      used for antigen detection of inactivated vaccine samples and clinical samples. 
      It is a novel method for diagnosing diseases and evaluation of the PEDV vaccine.
CI  - Copyright (c) 2020 Fan, Sun, Zhu, Zhou, Zhao, Yu, Sun, Guo, He and Li.
FAU - Fan, Baochao
AU  - Fan B
AD  - Key Laboratory of Veterinary Biological Engineering and Technology Ministry of 
      Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural 
      Sciences, Nanjing, China.
AD  - Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory 
      Cultivation Base of Ministry of Science and Technology, Nanjing, China.
AD  - Jiangsu Co-infection Center for Prevention and Control of Important Animal 
      Infectious Disease and Zoonoses, Yangzhou, China.
AD  - Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, China.
FAU - Sun, Jie
AU  - Sun J
AD  - Key Laboratory of Veterinary Biological Engineering and Technology Ministry of 
      Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural 
      Sciences, Nanjing, China.
AD  - Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory 
      Cultivation Base of Ministry of Science and Technology, Nanjing, China.
AD  - Jiangsu Co-infection Center for Prevention and Control of Important Animal 
      Infectious Disease and Zoonoses, Yangzhou, China.
AD  - Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, China.
FAU - Zhu, Lin
AU  - Zhu L
AD  - Key Laboratory of Veterinary Biological Engineering and Technology Ministry of 
      Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural 
      Sciences, Nanjing, China.
AD  - Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory 
      Cultivation Base of Ministry of Science and Technology, Nanjing, China.
AD  - Jiangsu Co-infection Center for Prevention and Control of Important Animal 
      Infectious Disease and Zoonoses, Yangzhou, China.
AD  - Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, China.
FAU - Zhou, Jinzhu
AU  - Zhou J
AD  - Key Laboratory of Veterinary Biological Engineering and Technology Ministry of 
      Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural 
      Sciences, Nanjing, China.
AD  - Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory 
      Cultivation Base of Ministry of Science and Technology, Nanjing, China.
AD  - Jiangsu Co-infection Center for Prevention and Control of Important Animal 
      Infectious Disease and Zoonoses, Yangzhou, China.
AD  - Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, China.
FAU - Zhao, Yongxiang
AU  - Zhao Y
AD  - Key Laboratory of Veterinary Biological Engineering and Technology Ministry of 
      Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural 
      Sciences, Nanjing, China.
AD  - Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory 
      Cultivation Base of Ministry of Science and Technology, Nanjing, China.
AD  - Jiangsu Co-infection Center for Prevention and Control of Important Animal 
      Infectious Disease and Zoonoses, Yangzhou, China.
AD  - Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, China.
FAU - Yu, Zhengyu
AU  - Yu Z
AD  - Key Laboratory of Veterinary Biological Engineering and Technology Ministry of 
      Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural 
      Sciences, Nanjing, China.
AD  - Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory 
      Cultivation Base of Ministry of Science and Technology, Nanjing, China.
AD  - Jiangsu Co-infection Center for Prevention and Control of Important Animal 
      Infectious Disease and Zoonoses, Yangzhou, China.
AD  - Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, China.
FAU - Sun, Bing
AU  - Sun B
AD  - Key Laboratory of Veterinary Biological Engineering and Technology Ministry of 
      Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural 
      Sciences, Nanjing, China.
AD  - Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory 
      Cultivation Base of Ministry of Science and Technology, Nanjing, China.
AD  - Jiangsu Co-infection Center for Prevention and Control of Important Animal 
      Infectious Disease and Zoonoses, Yangzhou, China.
AD  - Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, China.
FAU - Guo, Rongli
AU  - Guo R
AD  - Key Laboratory of Veterinary Biological Engineering and Technology Ministry of 
      Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural 
      Sciences, Nanjing, China.
AD  - Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory 
      Cultivation Base of Ministry of Science and Technology, Nanjing, China.
AD  - Jiangsu Co-infection Center for Prevention and Control of Important Animal 
      Infectious Disease and Zoonoses, Yangzhou, China.
AD  - Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, China.
FAU - He, Kongwang
AU  - He K
AD  - Key Laboratory of Veterinary Biological Engineering and Technology Ministry of 
      Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural 
      Sciences, Nanjing, China.
AD  - Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory 
      Cultivation Base of Ministry of Science and Technology, Nanjing, China.
AD  - Jiangsu Co-infection Center for Prevention and Control of Important Animal 
      Infectious Disease and Zoonoses, Yangzhou, China.
AD  - Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, China.
FAU - Li, Bin
AU  - Li B
AD  - Key Laboratory of Veterinary Biological Engineering and Technology Ministry of 
      Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural 
      Sciences, Nanjing, China.
AD  - Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory 
      Cultivation Base of Ministry of Science and Technology, Nanjing, China.
AD  - Jiangsu Co-infection Center for Prevention and Control of Important Animal 
      Infectious Disease and Zoonoses, Yangzhou, China.
AD  - Jiangsu Key Laboratory of Zoonoses, Yangzhou University, Yangzhou, China.
AD  - School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.
LA  - eng
PT  - Journal Article
DEP - 20201026
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC7649156
OTO - NOTNLM
OT  - PEDV
OT  - antigen detection
OT  - evaluation vaccine
OT  - intestinal and fecal samples
OT  - quantitative ELISA
EDAT- 2020/11/17 06:00
MHDA- 2020/11/17 06:01
PMCR- 2020/01/01
CRDT- 2020/11/16 08:55
PHST- 2020/04/07 00:00 [received]
PHST- 2020/10/01 00:00 [accepted]
PHST- 2020/11/16 08:55 [entrez]
PHST- 2020/11/17 06:00 [pubmed]
PHST- 2020/11/17 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2020.540248 [doi]
PST - epublish
SO  - Front Vet Sci. 2020 Oct 26;7:540248. doi: 10.3389/fvets.2020.540248. eCollection 
      2020.