PMID- 33198363 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240330 IS - 2076-3921 (Print) IS - 2076-3921 (Electronic) IS - 2076-3921 (Linking) VI - 9 IP - 11 DP - 2020 Nov 12 TI - MARGRA Lamb Eating Quality and Human Health-Promoting Omega-3 Long-Chain Polyunsaturated Fatty Acid Profiles of Tattykeel Australian White Sheep: Linebreeding and Gender Effects. LID - 10.3390/antiox9111118 [doi] LID - 1118 AB - Health-conscious consumers increasingly demand healthier, tastier, and more nutritious meat, hence the continuous need to meet market specifications and demand for high-quality lamb. We evaluated the longissimus dorsi muscle of 147 Tattykeel Australian White (TAW) sheep fed on antioxidant-rich ryegrass pastures exclusive to MAGRA lamb brand for meat eating quality parameters of intramuscular fat (IMF) content, fat melting point (FMP) and omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA). The aim was to assess the impact of linebreeding and gender on pasture-fed lamb eating quality and to test the hypothesis that variation in healthy lamb eating quality is a function of lamb gender and not its antioxidant status or inbreeding coefficient (IC). After solid-phase extraction and purification, phenolics and antioxidant enzyme activities were analysed by high-performance liquid chromatography and mass spectrometry. IMF and fatty acid composition were determined using solvent extraction and gas chromatography, respectively. IC was classified into low (0-5%), medium (6-10%) and high (>10%) and ranged from 0-15.6%. FMP and IMF ranged from 28 to 39 degrees C and 3.4% to 8.2%, with overall means of 34.6 +/- 2.3 degrees C and 4.4 +/- 0.2%, respectively, and n-3 LC-PUFA ranged from "source" to "good source" levels of 33-69 mg/100 g. Ewes had significantly (P < 0.0001) higher IMF, C22:5n-3 (DPA), C22:6n-3 (DHA), C18:3n-6, C20:3, C22:4n-6, C22:5n-6, total monounsaturated (MUFA), PUFA and Sigman-3 fatty acids and lower total saturated fatty acids (SFA) and FMP, than rams. As IC increased, there were no differences in FMP and IMF. Folin-Ciocalteu total phenolics, ferric reducing antioxidant power and antioxidant activities of glutathione peroxidase, catalase and superoxide dismutase enzymes did not differ by either gender or IC. This study provides evidence that IC is inconsequential in affecting antioxidant status, IMF, FMP and n-3 LC-PUFA in linebred and pasture-fed TAW sheep because the observed variation in individual fatty acids was mainly driven by gender differences between ewes and rams, hence the need to accept the tested hypothesis. This finding reinforces the consistent healthy eating quality of MARGRA lamb brand from TAW sheep regardless of its linebred origin. FAU - Pewan, Shedrach Benjamin AU - Pewan SB AUID- ORCID: 0000-0002-7254-0917 AD - Animal Genetics and Nutrition, Veterinary Sciences Discipline, College of Public Health, Medical and Veterinary Sciences, Division of Tropical Health and Medicine, James Cook University, Townsville, QLD 4811, Australia. AD - National Veterinary Research Institute, Private Mail Bag 01 Vom, Plateau State, Nigeria. FAU - Otto, John Roger AU - Otto JR AUID- ORCID: 0000-0002-6578-0787 AD - Animal Genetics and Nutrition, Veterinary Sciences Discipline, College of Public Health, Medical and Veterinary Sciences, Division of Tropical Health and Medicine, James Cook University, Townsville, QLD 4811, Australia. FAU - Kinobe, Robert Tumwesigye AU - Kinobe RT AUID- ORCID: 0000-0003-1166-0947 AD - Animal Genetics and Nutrition, Veterinary Sciences Discipline, College of Public Health, Medical and Veterinary Sciences, Division of Tropical Health and Medicine, James Cook University, Townsville, QLD 4811, Australia. FAU - Adegboye, Oyelola Abdulwasiu AU - Adegboye OA AUID- ORCID: 0000-0002-9793-8024 AD - Australian Institute of Tropical Health and Medicine, College of Public Health, Medical and Veterinary Sciences, Division of Tropical Health and Medicine, James Cook University, Townsville, QLD 4811, Australia. FAU - Malau-Aduli, Aduli Enoch Othniel AU - Malau-Aduli AEO AUID- ORCID: 0000-0002-3660-7862 AD - Animal Genetics and Nutrition, Veterinary Sciences Discipline, College of Public Health, Medical and Veterinary Sciences, Division of Tropical Health and Medicine, James Cook University, Townsville, QLD 4811, Australia. LA - eng GR - ICG000541/Innovation Connections Research Grant from the Australian Commonwealth Government's Dept of Industry, Innovation, Climate Change, Science, Research and Tertiary Education,/ PT - Journal Article DEP - 20201112 PL - Switzerland TA - Antioxidants (Basel) JT - Antioxidants (Basel, Switzerland) JID - 101668981 PMC - PMC7697536 OTO - NOTNLM OT - MARGRA lamb OT - Tattykeel Australian White OT - antioxidants OT - fat melting point OT - gender OT - inbreeding coefficient OT - intramuscular fat OT - longissimus dorsi muscle OT - meat quality OT - omega-3 LC-PUFA COIS- The authors declare no conflict of interest. The funders had no role in the design of the study; collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. EDAT- 2020/11/18 06:00 MHDA- 2020/11/18 06:01 PMCR- 2020/11/12 CRDT- 2020/11/17 01:06 PHST- 2020/10/16 00:00 [received] PHST- 2020/11/06 00:00 [revised] PHST- 2020/11/11 00:00 [accepted] PHST- 2020/11/17 01:06 [entrez] PHST- 2020/11/18 06:00 [pubmed] PHST- 2020/11/18 06:01 [medline] PHST- 2020/11/12 00:00 [pmc-release] AID - antiox9111118 [pii] AID - antioxidants-09-01118 [pii] AID - 10.3390/antiox9111118 [doi] PST - epublish SO - Antioxidants (Basel). 2020 Nov 12;9(11):1118. doi: 10.3390/antiox9111118.