PMID- 33198873
OWN - NLM
STAT- MEDLINE
DCOM- 20201119
LR  - 20201119
IS  - 2095-4352 (Print)
VI  - 32
IP  - 10
DP  - 2020 Oct
TI  - [Role of hydrogen sulfide on expression of phosphatidylinositol 3 kinase/protein 
      kinase B signal pathway in rats with intestinal ischemia/reperfusion injury].
PG  - 1247-1250
LID - 10.3760/cma.j.cn121430-20200417-00310 [doi]
AB  - OBJECTIVE: To explore the effect of hydrogen sulfide (H(2)S) on expression of 
      phosphatidylinositol 3 kinase/protein kinase B (PI3K/Akt) signal pathway in rats 
      with intestinal ischemia/reperfusion (IRI) injury. METHODS: Thirty male Wistar 
      rats were divided into sham operation group (Sham group), IRI group, and H(2)S 
      precursor sodium hydrosuphide (NaHS) intervention group (IRI+NaHS group) by 
      random number table method, with 10 rats in each group. The animal model of IRI 
      was established by 60 minutes superior mesenteric artery (SMA) blockage with 
      non-invasive vascular clamp and 120 minutes reflow. SMA was dissociated and 
      peritoneum cavity was closed in Sham group. The rats in IRI+NaHS group was 
      received NaHS (100 mumol/kg bolus+1.07 mmolxkg(-1)xh(-1) infusion) 10 minutes 
      prior to the onset of reperfusion, while the rats in IRI group and Sham group 
      were received equal volume of normal sodium. Blood in vena cava was collected. 
      H(2)S was detected by sensitive sulfide electrode. Rats were sacrificed after 
      blood collection. Histopathology change was observed by hematoxylin-eosin (HE) 
      staining, ileal pathological score was studied by Chiu score. The protein 
      expressions of phosphated Akt (p-Akt), Akt, PI3K, cleaved caspase-9, mammalian 
      target of rapamycin (mTOR) were determined by Western Blot. RESULTS: Compared 
      with the Sham group, there was intestinal mucosa structure disorder edema and 
      shedding villous fracture in the IRI group. Ileal pathological score in IRI group 
      was significantly increased (4.21+/-0.15 vs. 0.15+/-0.03, P < 0.01), while plasma 
      H(2)S in IRI group was significantly decreased (mumol/L: 26.72+/-3.17 vs. 
      38.34+/-5.24, P < 0.01). Ileal p-Akt, PI3K, caspase-9 and mTOR protein in IRI group 
      were significantly increased (p-Akt/GAPDH: 2.67+/-0.12 vs. 0.24+/-0.05, PI3K/GAPDH: 
      1.42+/-0.07 vs. 0.57+/-0.08, caspase-9/GAPDH: 4.23+/-0.61 vs. 0.13+/-0.02, mTOR/GAPDH: 
      2.17+/-0.23 vs. 0.23+/-0.02, all P < 0.01). Compared with the IRI group, pathological 
      changes of intestinal mucosa in the IRI+NaHS group was improved, ileal 
      pathological score was significantly decreased (1.56+/-0.02 vs. 4.21+/-0.15, P < 
      0.01), plasma H(2)S was significantly increased (mumol/L: 32.36+/-2.45 vs. 
      26.72+/-3.17, P < 0.01) and ileal p-Akt, PI3K were significantly increased 
      (p-Akt/GAPDH: 5.12+/-0.08 vs. 2.67+/-0.12, PI3K/GAPDH: 3.14+/-0.05 vs. 1.42+/-0.07, both 
      P < 0.01), while caspase-9, mTOR in IRI+NaHS group were significantly decreased 
      (caspase-9/GAPDH: 2.12+/-0.24 vs. 4.23+/-0.61, mTOR/GAPDH: 1.37+/-0.28 vs. 2.17+/-0.23, 
      both P < 0.01). CONCLUSIONS: H(2)S attenuates intestinal injury in IRI rats by 
      up-regulating PI3K/Akt signal pathway and down-regulating caspase-9 and mTOR.
FAU - Lu, Genlin
AU  - Lu G
AD  - First Department of General Surgery, Longyou County People's Hospital, Longyou 
      324400, Zhejiang, China.
FAU - Wu, Aibing
AU  - Wu A
AD  - Oncology Center, the Hospital Affiliated to Guangdong Medical University, 
      Zhanjiang 523808, Guangdong, China.
FAU - Wang, Hongbin
AU  - Wang H
AD  - Department of Hepatopancreato-biliary Surgery, the Hospital Affiliated to Qinghai 
      University, Xining 810001, Qinghai, China. Corresponding author: Lu Genlin, 
      Email: lugenlin007@163.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhonghua wei zhong bing ji jiu yi xue
JID - 101604552
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - YY9FVM7NSN (Hydrogen Sulfide)
SB  - IM
MH  - Animals
MH  - Hydrogen Sulfide
MH  - Male
MH  - Phosphatidylinositol 3-Kinase
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - *Reperfusion Injury
MH  - Signal Transduction
EDAT- 2020/11/18 06:00
MHDA- 2020/11/20 06:00
CRDT- 2020/11/17 05:48
PHST- 2020/11/17 05:48 [entrez]
PHST- 2020/11/18 06:00 [pubmed]
PHST- 2020/11/20 06:00 [medline]
AID - 10.3760/cma.j.cn121430-20200417-00310 [doi]
PST - ppublish
SO  - Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2020 Oct;32(10):1247-1250. doi: 
      10.3760/cma.j.cn121430-20200417-00310.