PMID- 33199992
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201118
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 20
IP  - 6
DP  - 2020 Dec
TI  - Effect of sericin on the p38MAPK signaling pathway and NLRP3 inflammasome in the 
      kidney of type 2 diabetic rats.
PG  - 267
LID - 10.3892/etm.2020.9397 [doi]
LID - 267
AB  - The present study aimed to investigate the effects of sericin on the p38MAPK 
      signaling pathway and nucleotide-binding oligomerization domain-like receptor 
      protein 3 (NLRP3) inflammasome in the kidney of rats with type 2 diabetes 
      mellitus (T2DM). A total of 36 male Sprague-Dawley rats were randomly divided 
      into the normal group, T2DM model group and sericin group (n=12 rats/group). A 
      T2DM model was developed through intraperitoneal injection of streptozotocin (35 
      mg.kg(-1).d(-1) for 2 consecutive days), and a high-fat and high-sugar diet. The 
      T2DM rats in the sericin group were administered 2.4 g.kg(-1).d(-1) sericin for 
      35 days, and rats in the other groups were administered an equal volume of normal 
      saline for 35 days. Fasting blood glucose was measured using the glucose oxidase 
      method. Kidney tissue morphology was observed by H&E staining. 
      Immunohistochemistry, western blotting, ELISA and reverse 
      transcription-quantitative PCR were used to detect the levels of MKK6, p38MAPK, 
      phosphorylated (p)-p38MAPK, NF-kappaB, IL-1beta, IL-6, NLRP3 and caspase-1 in rat kidney 
      tissues. The results revealed that blood glucose concentration, and the 
      expression levels of MKK6, p-p38MAPK, NF-kappaB, IL-1beta, IL-6, NLRP3 and caspase-1 
      were significantly increased in the T2DM group compared with those in the normal 
      group (P<0.05). In addition, obvious pathological changes were observed in the 
      T2DM group. Conversely, glucose concentration, and the expression levels of MKK6, 
      p-p38MAPK, NF-kappaB, IL-1beta, IL-6, NLRP3 and caspase-1 were significantly reduced in 
      the sericin group compared with those in the T2DM group (P<0.05). The 
      pathological changes were also obviously reduced. Notably, there was no 
      significant difference in p38MAPK expression among the three groups (P>0.05). 
      Collectively, the present study revealed that sericin may downregulate the 
      expression levels of MKK6, p-p38MAPK, NF-kappaB, IL-1beta, IL-6, NLRP3 and caspase-1, 
      and inhibit activation of renal p38MAPK signaling and NLRP3-associated 
      inflammation, which in turn may protect against kidney damage caused by T2DM.
CI  - Copyright: (c) Liu et al.
FAU - Liu, Donghui
AU  - Liu D
AD  - Department of Human Anatomy, Chengde Medical University, Chengde, Hebei 067000, 
      P.R. China.
FAU - Chen, Cheng
AU  - Chen C
AD  - Department of Physiology, Chengde Medical University, Chengde, Hebei 067000, P.R. 
      China.
FAU - Wang, Dandan
AU  - Wang D
AD  - Department of Anatomy, Shijiazhuang Medical College, Shijiazhuang, Hebei 050599, 
      P.R. China.
FAU - Chen, Zhihong
AU  - Chen Z
AD  - Department of Human Anatomy, Chengde Medical University, Chengde, Hebei 067000, 
      P.R. China.
FAU - Song, Chengjun
AU  - Song C
AD  - Department of Human Anatomy, Chengde Medical University, Chengde, Hebei 067000, 
      P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20201027
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC7664612
OTO - NOTNLM
OT  - NLRP3 inflammasome
OT  - T2DM
OT  - kidney
OT  - p38MAPK signaling pathway
OT  - sericin
EDAT- 2020/11/18 06:00
MHDA- 2020/11/18 06:01
PMCR- 2020/10/27
CRDT- 2020/11/17 06:22
PHST- 2020/02/25 00:00 [received]
PHST- 2020/07/31 00:00 [accepted]
PHST- 2020/11/17 06:22 [entrez]
PHST- 2020/11/18 06:00 [pubmed]
PHST- 2020/11/18 06:01 [medline]
PHST- 2020/10/27 00:00 [pmc-release]
AID - ETM-0-0-09397 [pii]
AID - 10.3892/etm.2020.9397 [doi]
PST - ppublish
SO  - Exp Ther Med. 2020 Dec;20(6):267. doi: 10.3892/etm.2020.9397. Epub 2020 Oct 27.