PMID- 33202146
OWN - NLM
STAT- MEDLINE
DCOM- 20210222
LR  - 20210222
IS  - 1535-4989 (Electronic)
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 64
IP  - 2
DP  - 2021 Feb
TI  - MicroRNA-574-5p Attenuates Acute Respiratory Distress Syndrome by Targeting 
      HMGB1.
PG  - 196-207
LID - 10.1165/rcmb.2020-0112OC [doi]
AB  - Acute respiratory distress syndrome (ARDS) is a critical condition with high 
      mortality. HMGB1 (high-mobility group protein B1) is one of the key 
      proinflammatory factors in the ARDS "inflammatory storm." According to previous 
      studies, some microRNAs (miRNAs) play important roles in this process. We aimed 
      to determine the contributing miRNAs targeting the expression and release of 
      HMGB1. miRNA expression in the peripheral blood of patients with ARDS was 
      measured by miRNA microarray. miRNAs targeting HMGB1 were screened and explored 
      for further study. In LPS-induced cell and mouse ARDS models, we explored the 
      effect of this miRNA on the expression and secretion of HMGB1 by Western blot, 
      real-time qPCR, and ELISA. The effects of this miRNA on the NF-kappaB signaling 
      pathway, proinflammatory cytokines, and NLRP3 (nod-like receptor protein 3) 
      inflammasome were detected by Western blot and real-time qPCR. In ARDS models, 
      microRNA-574-5p (miR-574-5p) expression could be induced by the TLR4/NF-kappaB 
      pathway upon LPS stimulation. It could suppress the inflammatory response by 
      targeting HMGB1. Enforcing the expression of miR-574-5p or HMGB1 siRNA silencing 
      inhibits the activation of NF-kappaB signaling pathway and the NLRP3 inflammasome. 
      Moreover, overexpression of HMGB1 reversed the antiinflammatory effect of 
      miR-574-5p. In ARDS mice, overexpression of miR-574-5p suppresses alveolar 
      leukocytes infiltration, interstitial edema, protein effusion, and inflammation. 
      This study demonstrated that miR-574-5p provided negative feedback to LPS-induced 
      inflammation and relieved ARDS. It may provide new therapeutic strategies for 
      ARDS.
FAU - He, Binchan
AU  - He B
AD  - Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical 
      School of Nanjing University, Nanjing, China.
AD  - The Second Affiliated Hospital of Nanjing University of Chinese Medicine, 
      Nanjing, China; and.
FAU - Zhou, Wei
AU  - Zhou W
AD  - Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical 
      School of Nanjing University, Nanjing, China.
FAU - Rui, Yuwen
AU  - Rui Y
AD  - Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical 
      School of Nanjing University, Nanjing, China.
FAU - Liu, Lulu
AU  - Liu L
AD  - Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical 
      School of Nanjing University, Nanjing, China.
FAU - Chen, Bilin
AU  - Chen B
AD  - Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical 
      School of Nanjing University, Nanjing, China.
FAU - Su, Xin
AU  - Su X
AD  - Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical 
      School of Nanjing University, Nanjing, China.
AD  - Department of Respiratory and Critical Care Medicine, Jinling Hospital, The First 
      School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (Cytokines)
RN  - 0 (HMGB1 Protein)
RN  - 0 (HMGB1 protein, mouse)
RN  - 0 (Inflammasomes)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (MIRN574 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (NF-kappa B)
SB  - IM
CIN - Am J Respir Cell Mol Biol. 2021 Feb;64(2):158-160. PMID: 33522886
MH  - Animals
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Endothelial Cells/metabolism
MH  - HMGB1 Protein/*metabolism
MH  - Humans
MH  - Inflammasomes/metabolism
MH  - Inflammation/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*metabolism
MH  - NF-kappa B/metabolism
MH  - Respiratory Distress Syndrome/*metabolism
MH  - Signal Transduction/physiology
PMC - PMC7874400
OTO - NOTNLM
OT  - HMGB1
OT  - NF-kappaB
OT  - NLRP3 inflammasome
OT  - miR-574-5p
EDAT- 2020/11/18 06:00
MHDA- 2021/02/23 06:00
PMCR- 2021/02/01
CRDT- 2020/11/17 20:05
PHST- 2020/11/18 06:00 [pubmed]
PHST- 2021/02/23 06:00 [medline]
PHST- 2020/11/17 20:05 [entrez]
PHST- 2021/02/01 00:00 [pmc-release]
AID - 10.1165/rcmb.2020-0112OC [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 2021 Feb;64(2):196-207. doi: 10.1165/rcmb.2020-0112OC.